中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7909-7913.doi: 10.3969/j.issn.2095-4344.2012.42.023

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

植入式皮质电刺激缺血性脑卒中大鼠皮质神经丝蛋白200的表达

程凌燕1，李 涛1，周海涵2，程 璇1，张 乾1，谭 杰1，李承晏1，段晏文2   

  1. 1武汉大学人民医院神经内一科，湖北省武汉市 430060
    2武汉大学化学与分子科学学院，湖北省武汉市 430072
  • 收稿日期:2011-12-29 修回日期:2012-02-14 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 段晏文，博士，教授，博士生导师，武汉大学化学与分子科学学院，湖北省武汉市 430072 yduan@whu.edu.cn
  • 作者简介:程凌燕★，女，1984年生，湖北省崇阳县人，汉族，武汉大学人民医院在读硕士，主要从事脑血管病及神经生物材料研究。 lingyan_0618@163.com

Effect of cortical electrical stimulation on neurofilament protein 200 expression in ischemic stroke rats

Cheng Ling-yan1, Li Tao1, Zhou Hai-han2, Cheng Xuan1, Zhang Qian1, Tan Jie1, Li Cheng-yan1, Duan Yan-wen2   

  1. 1First Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
    2College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, China
  • Received:2011-12-29 Revised:2012-02-14 Online:2012-10-14 Published:2012-10-14
  • Contact: Li Tao, Doctor, Associate professor, Master’s supervisor, First Department of neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China ltlll@163.com
  • About author:Cheng Ling-yan★, Studying for master's degree, First Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China lingyan_0618 @163.com

PDF

559

被引次数

4

摘要：

背景：前期研究中发现单独皮质电刺激治疗16 d能改善脑卒中后的运动功能障碍，增加梗死灶周围皮质微管相关蛋白2的表达。
目的：观察植入式皮质电刺激对缺血性脑卒中模型大鼠皮质神经丝蛋白200表达的影响。
方法：建立SD大鼠右侧大脑中动脉缺血性脑卒中模型，随机分成电刺激组和非刺激组，两组造模1周后植入电刺激器，电极放置在脑梗死区边缘皮质表面，电刺激组施加电刺激，非刺激组不施加电刺激。植入14 d后终止电刺激，6周后用免疫荧光染色和免疫荧光图像分析系统定量分析梗死灶周边、梗死对侧皮质神经丝蛋白200的表达水平。
结果与结论：与非刺激组比较，电刺激组梗死灶周边、梗死对侧皮质神经丝蛋白200表达水平均较高(P < 0.05)。表明皮质电刺激可增加梗死灶周边及梗死对侧皮质神经丝蛋白200的表达，诱导双侧皮质神经元轴突的可塑性改变。

关键词: 缺血性脑卒中, 皮质电刺激, 神经可塑性, 神经丝蛋白200, 神经元

Abstract:

BACKGROUND: Early studies have shown that cortical electrical stimulation (CES) alone for 16 days can enhance dyskinesia and the expression of microtubule-associated protein 2 (MAP2) in peri-infarct cortex after cerebral ischemia in rats.
OBJECTIVE: To investigate the effects of CES on the expression of neurofilament protein 200 (NF-200) in rats model of ischemic stroke.
METHODS: Sprague-Dawley rats model of right middle cerebral artery occlusion in ischemic stroke were established. The rats with lesions in the cortex were randomly divided into CES group (n=13) and non-stimulation group (n=10). One week after modeled, electrical stimulators were implanted and the electrodes were placed on the surface of peri-infarct cortex. CES group was given the electric stimulation, but not in the non-stimulation group. CES was terminated after implantation for 14 days. And then the expression of NF-200 in the peri-infarct and contralateral cortex was assessed by immunofluorescence image analysis at 6 weeks after CES started.
RESULTS AND CONCLUSION: In the peri-infarct and contralateral cortex, the expression of NF-200 in the CES group was higher than that in the non-stimulation group (P < 0.05). These results suggest that CES can increase the expression of NF-200 in both peri-infarct and contralateral cortex, and induce the plasticity of bilateral cortical neuron axons.

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