中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7068-7072.doi: 10.3969/j.issn.2095-4344.2012.38.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

金纳米棒的表面改性及生物相容性

余金妹1，高 斌1，贺克武1，程继新1，鲍 乐2，黄永翠1，李劲松1，任子甲3，肖卫华3   

  1. 1安徽医科大学第三附属医院，安徽省合肥市 230061
    2徐州医学院，影像学院，江苏省徐州市 221004
    3中国科学技术大学生命科学学院，安徽省合肥市 230027
  • 收稿日期:2012-03-16 修回日期:2012-05-06 出版日期:2012-09-16 发布日期:2012-09-16
  • 通讯作者: 高斌，主任医师，硕士生导师，安徽医科大学第三附属医院影像中心，安徽省合肥市 230061 gaobin_3136@ 163.com 并列通讯作者：肖卫华，教授，博士生导师，中国科学技术大学生命科学院，安徽省合肥市 230027 xiaow@ ustc.edu.cn
  • 作者简介:余金妹★，女，1984年生，安徽省合肥市人，汉族，安徽医科大学在读硕士，主要从事影像医学与核医学研究。 jinmei_yu3733@163.com

Surface modification and biocompatibility of gold nanorods

Yu Jin-mei1, Gao Bin1, He Ke-wu1, Cheng Ji-xin1, Bao Le2, Huang Yong-cui1, Li Jin-song1, Ren Zi-jia3, Xiao Wei-hua3   

  1. 1Third Affiliated Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China
    2Department of Medical Imaging, Xuzhou Medical School, Xuzhou 2210043, Jiangsu Province, China
    3School of Life Science, University of Science and Technology of China, Hefei 230027, Anhui Province, China
  • Received:2012-03-16 Revised:2012-05-06 Online:2012-09-16 Published:2012-09-16
  • Contact: Gao Bin, Chief physician, Master’s supervisor, Third Affiliated Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China gaobin_3136@ 163.com Xiao Wei-hua, Professor, Doctoral supervisor, School of Life Science, University of Science and Technology of China, Hefei 230027, Anhui Province, China xiaow@ustc.edu.cn
  • About author:Yu Jin-mei★, Studying for master’s degree, Third Affiliated Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China jinmei_yu3733@ 163.com

PDF

669

摘要：

背景：金纳米棒制备过程中加入的十六烷基三甲基溴化铵可促进棒状金纳米颗粒的形成，保证金纳米棒良好的分散性，但也会产生一定毒性。
目的：评价表面修饰后金纳米棒的生物相容性。
方法：运用种子介导生长法制备金纳米棒，以改良后的奥伯法对其表面进行二氧化硅修饰，采用紫外分光光度计和透射电子显微镜对其进行表征，将修饰后的金纳米棒配制成不同浓度(60，45，30，15 μmol/L)，分别与肝癌bel-7402细胞及胃癌MGC-803细胞孵育，采用CCK-8法检测细胞相对增殖率，细胞毒性级别。
结果与结论：表面修饰后的金纳米棒分散性好，其特殊的光学性质未有改变，分别在500，825 nm处有强吸收峰。不同浓度金纳米棒处理后，肝癌bel-7402细胞的相对增殖率在95%-100%之间，胃癌MGC-803细胞的相对增殖率在99%-103%之间，两组细胞的毒性评级均为0级或1级，无细胞毒性。表明经二氧化硅表面修饰后的金纳米棒具有良好的生物相容性。

关键词: 金纳米棒, 表面修饰, 二氧化硅, 生物相容性, 肝癌, 胃癌, 改性, 生物材料

Abstract:

BACKGROUND: The added cetyl trimethyl ammonium bromide molecules in the process of the preparation of gold nanorods can promote the formation of rodlike gold nanoparticles, and ensure the dispersion of gold nanorods. However, it can also have certain toxicity.
OBJECTIVE: To evaluate the biocompatibility of surface modified gold nanorods.
METHODS: Gold nanorods were synthesized by the method of seed-mediated growth and coated with silica by a modified Stober method. The physical properties including shapes, disperbility and absorption peaks of the silica-coated gold nanorods were characterized by transmission electron microscopy and UV-vis spectrometer respectively. The coated gold nanorods were prepared in solution at different concentrations (60, 45, 30, 15 μmol/L) and then were incubated by liver cancer bel-7402 cell line or stomach cancer MGC-803 cell line. After that, cell proliferation and cytotoxicity grading were detected by the cell counting kit-8 method.
RESULTS AND CONCLUSION: The silica-coated gold nanorods had a good dispersivity; its optical properties had not changed, and conserved absorption peaks at 500 nm and 825 nm, respectively. After co-cultured with the gold nanorods, the relative growth rate of liver cancer bel-7402 cell line was between 95% and 100%, while that of stomach cancer MGC-803 cell line was between 99% and 103%. The toxicity grading of the two kinds of cells was level 0 or 1, respectively. In addition, they had no cytotoxicity. These results suggest that the gold nanorods coated with silica have good biocompatibility.

中图分类号: