中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (36): 6785-6789.doi: 10.3969/j.issn.2095-4344.2012.36.024

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

血小板微粒对神经干细胞增殖和分化的影响

徐 颖1，陆士奇1，李军根2，孙海伟1   

  1. 苏州大学附属第一医院，1急诊内科，2急诊外科，江苏省苏州市 215006
  • 收稿日期:2012-06-09 修回日期:2012-07-10 出版日期:2012-09-02 发布日期:2012-09-02
  • 通讯作者: 陆士奇，博士，主任医师，苏州大学附属第一医院急诊内科，江苏省苏州市 215006 papersub@sina.com
  • 作者简介:徐颖★，女，1976年生，江苏省苏州市人，汉族，硕士，主治医师，主要从事干细胞治疗神经损伤的研究。 ava1105@163.com

Effects of platelet microparticles on proliferation and differentiation of neural stem cells

Xu Ying1, Lu Shi-qi1, Li Jun-gen2, Sun Hai-wei1   

  1. 1Department of Emergency Internal Medicine, 2Department of Emergency Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Received:2012-06-09 Revised:2012-07-10 Online:2012-09-02 Published:2012-09-02
  • Contact: Lu Shi-qi, M.D., Chief physician, Department of Emergency Internal Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China papersub@sina.com
  • About author:Xu Ying★, Master, Attending physician, Department of Emergency Internal Medicine, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China ava1105@163.com

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摘要：

背景：血小板微粒是血小板在活化过程中释放的小的亚细胞碎片，在血管新生和缺血组织新生中发挥作用。
目的：观察血小板微粒对神经干细胞增殖、存活和分化的影响。
方法：取胎龄13.5 d的小鼠获取鼠胚胎神经干细胞，分别应用10 μg/L成纤维细胞生长因子、血管内皮生长因子或者0.1，1，10 μg的血小板微粒进行处理观察神经球的大小及细胞转归。
结果与结论：与单独应用成纤维细胞生长因子、血管内皮生长因子干预的神经干细胞相比，经血小板微粒处理的神经干细胞神经球直径明显增大，存活率明显增高。且血小板微粒可促进神经干细胞向胶质细胞和神经元分化。说明血小板微粒同其他生长因子一样均能促进神经干细胞的增殖、存活和分化，且效果优于生长因子单独应用。

关键词: 血小板微粒, 血管再生, 神经干细胞, 增殖, 分化, 生长因子, 神经元, 神经再生

Abstract:

BACKGROUND: Platelet microparticles are the small subcellular fragments released during the process of platelet activation and affect angiogenesis and postischemic tissue regeneration.
OBJECTIVE: To investigate the effects of platelet microparticles on proliferation, survival and activation of neural stem cells.
METHODS: Mouse embryonic neural stem cells were harvested from mouse fetus at gestational 13.5 days and treated with 10 μg/L fibroblast growth factor, vascular endothelial growth factor or 0.1, 1, 10 μg platelet microparticles to evaluate neurosphere size and cell fate.
RESULTS AND CONCLUSION: Compared with fibroblast growth factor- or vascular endothelial growth factor-treated neural stem cells, the platelet microparticles-treated neural stem cells exhibited a larger cell diameter and higher survival rate. Platelet microparticles could promote the differentiation of neural stem cells into glial cells and neurons. These findings suggest that platelet microparticles can better promote the proliferation, survival and differentiation of neural stem cells than other growth factors.

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