中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (34): 6271-6276.doi: 10.3969/j.issn.2095-4344.2012.34.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

成骨生长肽促进钛表面大鼠成骨细胞的增殖分化

彭 涛1，黄 姣2，徐 凌2   

  1. 1重庆市黔江中心医院，重庆市409000；
    2重庆医科大学附属口腔医院，重庆市口腔疾病与生物医学研究中心，重庆市 401147
  • 收稿日期:2012-06-28 修回日期:2012-07-12 出版日期:2012-08-19 发布日期:2012-08-19
  • 通讯作者: 徐凌，副教授，重庆医科大学附属口腔医院，重庆市口腔疾病与生物医学研究中心，重庆市 401147 h1201@163.com
  • 作者简介:彭涛，男，1974年生，重庆市人，土家族，2001年四川大学华西口腔医学院毕业，主治医师，主要从事口腔材料学及口腔组织工程学方面研究。

Osteoblastic growth peptides induce the proliferation and differentiation of rat osteoblast-like cells on titanium surface in vitro

Peng Tao1, Huang Jiao2, Xu Ling2   

  1. 1Qianjiang Central Hospital of Chongqing City, Chongqing 409000, China;
    2Chongqing Research Center for Oral Diseases and Biomedical Science, Affiliate Dental Hospital of Chongqing Medical University, Chongqing 401147, China
  • Received:2012-06-28 Revised:2012-07-12 Online:2012-08-19 Published:2012-08-19
  • Contact: Xu Ling, Associate professor, Chongqing Research Center for Oral Diseases and Biomedical Science, Affiliate Dental Hospital of Chongqing Medical University, Chongqing 401147, China xh1201@163.com
  • About author:Peng Tao, Attending physician, Qianjiang Central Hospital of Chongqing City, Chongqing 409000, China

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404

被引次数

6

摘要：

背景：成骨生长肽具有促进多种基质细胞增殖活性，成骨活性，免疫原性低，自身调节极其敏感，提取制作工艺简单等众多优点。
目的：体外观察成骨生长肽对大鼠颅盖骨来源成骨细胞在钛金属表面增殖分化的影响。
方法：将体外培养的新生SD大鼠颅盖骨成骨细胞以5×107 L-1细胞浓度接种于6孔板中的纯钛试件表面，分别加入0(空白对照)，10-10，10-9，10-8，10-7 mol/L的成骨生长肽，干预1，3，5，7，9 d后应用MTT法检测纯钛试件表面成骨细胞的增殖活性，应用酶联免疫法检测成骨细胞内碱性磷酸酶活性。
结果与结论：与空白对照组比较，各浓度成骨生长肽组纯钛试件表面成骨细胞增殖活跃(P < 0.05)，且最佳作用浓度为10-9 mol/L(P < 0.05)；各浓度成骨生长肽组细胞内碱性磷酸酶活性增强(P < 0.05)，且最佳作用浓度为10-8 mol/L (P < 0.05)。表明成骨生长肽可促进钛片表面成骨细胞的增殖活性，增强细胞内碱性磷酸酶活性。

关键词: 成骨生长肽, 钛, 成骨细胞, 细胞增殖, 碱性磷酸酶, 生物材料

Abstract:

BACKGROUND: Osteogenic growth peptides have many advantages, such as low immunogenicity, self regulating sensitivity and simple extraction and making process, which can promote the proliferation activity and osteogenic activity of a variety of stromal cells.
OBJECTIVE: To observe the effect of osteogenic growth peptides on proliferation and differentiation of rat cranium derived osteoblasts on titanium surface in vitro.
METHODS: Sprague Dawley rat cranium derived osteoblasts at a concentration of 5×107/L were cultured in vitro and located on the surface of titanium disc in the 6-well plate, then the osteogenic growth peptide at concentrations of 0 (blank control), 10-10, 10-9, 10-8 and 10-7 mol/L was added. The proliferative activity was examined by MTT, and alkaline phosphatase activity was examined by enzyme-linked immunosorbent assay individually after induced for 1, 3, 5, 7 and 9 days.
RESULTS AND CONCLUSION: Compared with the blank control group, the proliferation of the osteoblasts on the surface of titanium in different osteogenic growth peptide groups was increased (P < 0.05), and the best concentration of osteogenic growth peptide was 10-9 mol/L (P < 0.05); the alkaline phosphatase activity in different osteogenic growth peptide groups was increased (P < 0.05), and the best concentration of osteogenic growth peptide was 10-8 mol/L (P < 0.05). Our results demonstrate that osteogenic growth peptides can stimulate proliferative activity and alkaline phosphatase activity of rat osteoblasts located on titanium surface in vitro.

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