中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6173-6178.doi: 10.3969/j.issn.2095-4344.2012.33.019

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

两种脑性瘫痪鼠模型的对比

贺媛媛1，栾 佐2，唐久来1，杨印祥2，郝庆飞2，杜庆安2，卢雅彬2，宁浩勇3   

  1. 1安徽医科大学第一附属医院儿科，安徽省合肥市 230000；解放军海军总医院，2儿科， 3病理科，北京市 100048
  • 收稿日期:2012-02-21 修回日期:2012-05-08 出版日期:2012-08-12 发布日期:2012-08-12
  • 通讯作者: 栾佐，硕士，主任医师，解放军海军总医院儿科，北京市 100048 luanzuo@yahoo.com.cn
  • 作者简介:贺媛媛★，女，1985年生，汉族，河北省邢台市人，安徽医科大学在读硕士，主要从事儿科神经学的研究。 yyh1001101110good@163.com

Comparison of two types of cerebral palsy models in rats

He Yuan-yuan1, Luan Zuo2, Tang Jiu-lai1, Yang Yin-xiang2, Hao Qing-fei2, Du Qing-an2, Lu Ya-bin2, Ning Hao-yong3   

  1. 1Department of Pediatrics, First Affiliated Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China;
    2Department of Pediatrics, 3Department of Pathology, Chinese PLA Navy General Hospital, Beijing 100048, China
  • Received:2012-02-21 Revised:2012-05-08 Online:2012-08-12 Published:2012-08-12
  • Contact: Luan Zuo, Master, Chief physician, Department of Pediatrics, Chinese PLA Navy General Hospital, Beijing 100048, China luanzuo@yahoo.com.cn
  • About author:He Yuan-yuan★, Studying for master’s degree, Department of Pediatrics, First Affiliated Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China yyh1001101110good@163.com

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409

被引次数

4

摘要：

背景：现阶段主要围绕宫内窘迫，围生期缺氧缺血，胆红素脑病以及生后感染因素来构建脑性瘫痪模型，脑损伤后遗症不明显，联合损伤构建模式被逐渐认识。
目的：采用孕后期宫内感染及缺氧联合序贯缺氧缺血构建脑性瘫痪大鼠模型，对这些模型鼠进行评估，比较存在优势。
方法：孕鼠随机分为实验1组、实验2组和对照组。实验组孕16 d行腹腔脂多糖注射并缺氧2.5 h，对照组行生理盐水腹腔注射，隔日1次，直至分娩，所生幼鼠与其母鼠属同一组。实验1组幼鼠生后7 d行双侧颈总动脉结扎。生后14和28 d分别行体质量、爬梯、mNSS评分、旷场实验和病理切片。
结果与结论：实验1组动物体质量显著低于其他两组(P < 0.01)，爬梯掉下来的次数及MNSS评分均显著高于对照组(P < 0.05，P < 0.01)，28 d爬行距离最短，各项检测均提示生长发育迟缓，肌力肌张力不稳定，正常反射出现较迟(P < 0.05)，病理切片提示灰质神经元排列紊乱，白质层变薄，炎性细胞浸润。实验2组幼鼠脑损伤程度较实验1组轻，后遗症状不明显。提示孕后期脂多糖注射及缺氧合并生后双侧颈总动脉结扎可以制备大鼠脑性瘫痪模型并能稳定至生后4周。

关键词: 脑损伤, 感染, 脑性瘫痪, 缺氧缺血, 行为学, 大鼠, 动物模型

Abstract:

BACKGROUND: At present, studies about cerebral palsy models mainly focus on fetal distress, perinatal hypoxia-ischemia, bilirubin encephalopathy and infection; however, there is no obvious brain damage sequela. Combined fetal exposure to lipopolysaccharide and early neonatal hypoxia/ischemia is a new way to construct cerebral palsy models.
OBJECTIVE: To construct a novel type of cerebral palsy model in rats induced by intrauterine infection at late pregnancy and hypoxia combined with sequential hypoxia-ischemia and to evaluate the advantages of this model.
METHODS: All pregnant rats and their later offspring were divided into experimental group 1, experimental group 2 and control group. The pregnant rats were injected with lipopolysaccharide (i.p.) in the experimental groups and normal saline in the control group every two days, from pregnant day 16 until delivery. Four hours after injection, the experimental groups were exposed to O2/N2 mixture for 2.5 hours. On postnatal day 7, bilateral common carotid arteries were ligated in the experimental group 1. On postnatal days 14 and 28, immature rats in the three groups were tested by weight, crawling ladder, modified neurological severity scores and open-field test and pathological section.
RESULTS AND CONCLUSION: In the experimental group 1, the body mass was significantly lower than that in the other two groups (P < 0.01), and the number of drop down in crawling ladder test and modified neurological severity scores were significantly higher than that in the other two groups (P < 0.05, P < 0.01). The distance in crawling ladder test and open field test was the shortest in the experimental group 1 at day 28. The rats in the experimental group 1 showed slow growth and development, instable muscle force and delayed reflection (P < 0.05). Neurons in the gray matter arranged in disorder, the white matter became thinner and inflammatory cells infiltrated. The rats in the experimental group 2 were not as serious as those in the experimental group 1. These findings indicate that, in late pregnancy, intraperitoneal injection of lipopolysaccharide and hypoxia with postnatal bilateral common carotid artery ligation can build a new stable rat model of cerebral palsy.

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