中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6179-6183.doi: 10.3969/j.issn.2095-4344.2012.33.020

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

负压吸引与大鼠损伤坐骨神经的修复和再生

刘 华1，王平山2，解 琛2，徐 斌1，曹学成2   

  1. 1辽宁医学院解放军济南军区总医院研究生培养基地，山东省济南市 250031；
    2解放军济南军区总医院骨创科，山东省济南市 250031
  • 收稿日期:2012-04-06 修回日期:2012-05-16 出版日期:2012-08-12 发布日期:2013-07-15
  • 通讯作者: 王平山，博士，主任医师，硕士生导师，解放军济南军区总医院骨创伤外科，山东省济南市 250031 Wangpingshan@yahoo.com.cn
  • 作者简介:刘华★，男，1981年生，山东省滕州市人，汉族，辽宁医学院在读硕士，主要从事创伤骨科及手足显微外科研究。 81288328@163.com

Relationship of vacuum aspiration with repair and regeneration of rat injured sciatic nerve

Liu Hua1, Wang Ping-shan2, Xie Chen2, Xu Bin1, Cao Xue-cheng2   

  1. 1Postgraduate Training Base, the General Hospital of Jinan Military Area Command of Chinese PLA, Liaoning Medical University, Jinan 250031, Shandong Province, China;
    2Department of Bone, the General Hospital of Jinan Military Area Command of Chinese PLA, Jinan 250031, Shandong Province, China
  • Received:2012-04-06 Revised:2012-05-16 Online:2012-08-12 Published:2013-07-15
  • Contact: Wang Ping-shan, Doctor, Chief physician, Master’s supervisor, Department of Bone, the General Hospital of Jinan Military Area Command of Chinese PLA, Jinan 250031, Shandong Province, China Wangpingshan@yahoo.com.cn
  • About author:Liu Hua★, Studying for master’s degree, Postgraduate Training Base, the General Hospital of Jinan Military Area Command of Chinese PLA, Liaoning Medical University, Jinan 250031, Shandong Province, China 81288328@163.com

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摘要：

背景：外周神经缺损的修复是目前创伤外科及修复重建外科临床上的一个难题，常用的神经移植、神经延长、神经桥接和组织工程等方法有局限性。
目的：比较不同负压对大鼠损伤坐骨神经的修复和再生。
方法：健康成年SD 大鼠分别以 6.65，13.30，19.95 kPa压力对大鼠右侧离断坐骨神经近端行负压吸引。负压每吸引60 min，休息30 min，交替进行，连续4周。
结果与结论：术后1个月，6.65，13.30，19.95 kPa负压吸引的大鼠坐骨神经实验侧近端均有不同程度生长，且 13.30 kPa压力组生长长度明显优于6.65和19.95 kPa组；对延长的神经进行组织学观察，发现延长神经的近侧部分轴突轴索较直，弯曲度较小，粗细均匀，髓鞘连续性良好，再生神经生长较好；中段部分神经纤维致密，成簇状排列，神经延长末端髓鞘成分减少，许旺细胞增殖明显。说明负压吸引可以促进大鼠坐骨神经的再生，且13.30 kPa压力下更有利于神经再生。

关键词: 负压, 坐骨神经, 神经再生, 神经延长, 神经损伤, 动物模型, 大鼠

Abstract:

BACKGROUND: Repair of peripheral nerve defects is the current challenge in traumatic surgery and reconstructive surgery. The present hotspot in this field is how to promote neural regeneration. Several common methods have their own defects or limitations, such as nerve transplantation, nerve elongation, nerve bridging and tissue engineering.
OBJECTIVE: To compare the effect of vacuum aspiration on promoting the repair and regeneration of rat sciatic nerve at different pressures.
METHODS: Thirty healthy adult SD rats were divided into three groups following different pressures (6.65, 13.30 and 19.95 kPa) on the proximal end of the right sciatic nerve after transection in rats sucked by vacuum aspiration. After vacuum aspiration for 60 minutes, there was a rest of 30 minutes.
RESULTS AND CONCLUSION: After treatment for 1 month, the proximal nerve at the experimental side was found different degrees of growth. Moreover, growth length in 13.30 kPa group was longer than that in 6.65 and 19.95 kPa groups. Histological observation showed that in the proximal end of regenerated nerves, neuraxis was straight, little curved, and thickness was uniform, besides, the myelin sheath was well consecutive, and the growth of regenerated nerves was good. In the middle part, nerve fibers were compact and arranged in cluster. In the distal end, myelin sheath components were reduced, and hyperplasia of schwann cells could be observed obviously. These results suggest that vacuum aspiration can promote the regeneration of rat sciatic nerve. In addition, vacuum aspiration at 13.30 kPa exhibits more effective for nerve regeneration.

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