中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (35): 9128-9134.doi: 10.12307/2026.464

• 骨组织构建 bone tissue construction • 上一篇    下一篇

补肾健脾活血方改善去卵巢大鼠骨质流失及骨小梁生物力学参数

林  适1，2，杨彬彬1，3，4，唐子佳1，东智卓玛1，吴建军1，陈桐莹1，万  雷5，黄宏兴5，黄  红1，6   

  1. 1广州中医药大学，广东省广州市   510006；2广州中医药大学第一附属医院，广东省广州市   510405；3广州中医药大学附属深圳市骨伤科医院，广东省深圳市   518118；4深圳平乐骨伤科医院(深圳市坪山区中医院)，广东省深圳市   518118；5广州中医药大学第三附属医院，广东省广州市   510378；6广州中医药大学护理学院，广东省广州市   510006
  • 收稿日期:2025-10-22 修回日期:2026-02-14 出版日期:2026-12-18 发布日期:2026-04-25
  • 通讯作者: 黄宏兴，教授，广州中医药大学第三附属医院，广东省广州市 510378 通讯作者：黄红，正高级实验师，广州中医药大学，广东省广州市 510006；广州中医药大学护理学院，广东省广州市 510006
  • 作者简介:林适，男，1991年生，广东省阳春市人，汉族，博士，主治医师，主要从事中医药治疗骨质疏松症及中医药治疗骨伤科疾病的研究。
  • 基金资助:
    国家自然科学基金项目(82274551)，项目负责人：黄宏兴；广东省基础与应用基础研究基金企业联合基金重点项目(2023B1515230001)，项目负责人：黄宏兴；广东省科技计划省社会发展科技协同创新体系建设项目(2024A1111120018)，项目负责人：万雷

Bushen Jianpi Huoxue Formula inhibits bone loss and improves trabecular biomechanical parameters in ovariectomized rats

Lin Shi1, 2, Yang Binbin1, 3, 4, Tang Zijia1, Dongzhi Zhuoma1, Wu Jianjun1, Chen Tongying1, Wan Lei5, Huang Hongxing5, Huang Hong1, 6   

  1. 1Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China; 2The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; 3Shenzhen Orthopedics and Traumatology Hospital affiliated to Guangzhou University of Chinese Medicine, Shenzhen 518118, Guangdong Province, China; 4Shenzhen Pingle Orthopedic Hospital (Shenzhen Pingshan District Hospital of Traditional Chinese Medicine), Shenzhen 518118, Guangdong Province, China; 5The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510378, Guangdong Province, China; 6Nursing School of Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China
  • Received:2025-10-22 Revised:2026-02-14 Online:2026-12-18 Published:2026-04-25
  • Contact: Huang Hongxing, Professor, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510378, Guangdong Province, China Co-corresponding author: Huang Hong, Senior experimentalist, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China; Nursing School of Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China
  • About author:Lin Shi, PhD, Attending physician, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 82274551 (to HHX); the Key Project of Guangdong Provincial Basic and Applied Basic Research Foundation Enterprise Joint Fund, No. 2023B1515230001 (to HHX); the Guangdong Provincial Science and Technology Plan for Social Development Science and Technology Collaborative Innovation System Construction, No. 2024A1111120018 (to WL)

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摘要：



文题释义：
骨质疏松症：是一种以骨量低下、骨组织微结构损坏，导致骨脆性增加、易发生骨折为特征的全身性骨病，多见于绝经后女性和老年男性，与衰老、激素水平下降、钙和维生素 D 缺乏等有关，可致腰背痛、身高变矮，需早防早治。
有限元分析：在骨质疏松领域，有限元分析通过构建松质骨、皮质骨的多孔微结构模型模拟骨量流失后力学性能变化，可分析椎体压缩骨折时骨小梁应力集中区域，评估骨密度降低对髋关节承重能力的影响，还能优化抗骨质疏松药物干预或植入物设计，为骨强度预测及防治策略提供力学数据支撑。

背景：前期研究证实，补肾活血健脾方可提高骨质疏松症大鼠骨密度。
目的：探索补肾健脾活血方对去卵巢大鼠骨质流失及骨小梁生物力学参数的影响。 
方法：将50只雌性SD大鼠随机分5组干预：正常组(n=10)不进行任何干预，假手术组(n=10)切除卵巢附近的脂肪组织，去势组(n=10)、补肾健脾活血方组(n=10)、阿仑膦酸钠组(n=10)切除双侧卵巢建立骨质疏松模型；造模12周后，补肾健脾活血方组、阿仑膦酸钠组分别灌胃给予补肾健脾活血方、阿仑膦酸钠，其他3组灌胃给予生理盐水，每天1次，连续给药12周。给药结束后，检测腰椎骨密度，micro-CT分析股骨远端及胫骨近端骨微结构参数，苏木精-伊红观察胫骨近端组织学形态，有限元分析股骨远端应变与应变。
结果与结论：①去势组腰椎骨密度低于正常组、假手术组(P < 0.05)，补肾健脾活血方组、阿仑膦酸钠组腰椎骨密度高于去势组(P < 0.05)；②micro-CT分析显示，与正常组、假手术组比较，去势组胫骨近端(或股骨远端)骨体积分数、骨表面密度、骨小梁数量均降低(P < 0.05)，骨小梁分离度、结构模型指数均升高(P < 0.05)；与去势组比较，补肾健脾活血方组、阿仑膦酸钠组股骨远端的骨体积分数、骨表面密度、骨小梁数量均升高(P < 0.05)，股骨远端的骨小梁分离度、结构模型指数均降低(P < 0.05)，胫骨近端的骨体积分数、骨表面密度与骨小梁数量均升高(P < 0.05)；③苏木精-伊红染色结果显示，去势组骨骼髓腔脂肪浸润程度高于正常组、假手术组，补肾健脾活血方组、阿仑膦酸钠组骨髓髓腔脂肪浸润程度明显得到改善；④有限元分析显示，相同载荷下，去势组骨小梁应力、应变高于正常组、假手术组(P < 0.05)，补肾健脾活血方组、阿仑膦酸钠组骨小梁应力、应变低于去势组(P < 0.05)。结果表明：补肾健脾活血方干预去卵巢大鼠可防止骨质流失、提升骨小梁生物力学性能，具有预防骨质疏松症骨折发生的潜在疗效。

https://orcid.org/0000-0001-6388-071X (林适) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 骨质疏松症, 去势大鼠, 骨流失, 补肾健脾活血方, 生物力学, 骨小梁生物力学参数

Abstract: BACKGROUND: Previous studies have confirmed that the Bushen Jianpi Huoxue Formula can increase bone mineral density in osteoporotic rats.
OBJECTIVE: To investigate the effects of the Bushen Jianpi Huoxue Formula on bone loss and trabecular biomechanical parameters in ovariectomized rats.
METHODS: Fifty female Sprague-Dawley rats were randomly divided into five groups: a normal group (n=10) without any intervention, a sham-operated group (n=10) undergoing resection of adipose tissue near the ovaries, and an ovariectomized group (n=10), a Bushen Jianpi Huoxue Formula group (n=10), and an alendronate group (n=10) undergoing bilateral ovariectomy to establish an osteoporosis model. Twelve weeks after modeling, the Bushen Jianpi Huoxue Formula group and the alendronate group were orally administered the Bushen Jianpi Huoxue Formula and alendronate, respectively, while the other three groups were orally administered normal saline, once daily for 12 consecutive weeks. After final treatment, lumbar spine bone mineral density was measured, micro-CT was used to analyze bone microstructure parameters of the distal femur and proximal tibia, hematoxylin-eosin staining was used to observe the histological morphology of the proximal tibia, and finite element analysis was used to analyze strain and stress in the distal femur.
RESULTS AND CONCLUSION: (1) Lumbar spine bone mineral density in the ovariectomized group was lower than that in the normal and sham-operated groups (P < 0.05), while lumbar spine bone mineral density in the Bushen Jianpi Huoxue Formula group and the alendronate group was higher than that in the ovariectomized group (P < 0.05). (2) Micro-CT analysis showed that compared with the normal and sham-operated groups, the ovariectomized group exhibited significantly decreased bone volume fraction, bone surface density, and trabecular number (P < 0.05), as well as significantly increased trabecular separation and structure model index (P < 0.05) in the proximal tibia (or distal femur). Compared with the ovariectomized group, the Bushen Jianpi Huoxue formula and alendronate groups showed significantly increased bone volume fraction, bone surface density, and trabecular number in the distal femur (P < 0.05), significantly decreased trabecular separation and structure model index in the distal femur (P < 0.05), and significantly increased bone volume fraction, bone surface density, and trabecular number in the proximal tibia (P < 0.05). (3) Hematoxylin-eosin staining results showed that the degree of fatty infiltration in the bone marrow cavity was significantly higher in the ovariectomized group than in the normal and sham-operated groups. The Bushen Jianpi Huoxue Formula and alendronate groups showed marked improvement in bone marrow adiposity. (4) Finite element analysis showed that under the same load, trabecular stress and strain in the ovariectomized group were significantly higher than those in the normal and sham-operated groups (P < 0.05). The Bushen Jianpi Huoxue formula and alendronate groups exhibited significantly improved trabecular stress and strain compared with the ovariectomized group (P < 0.05). In conclusion, the Bushen Jianpi Huoxue Formula can prevent bone loss, improve bone microstructure, and enhance trabecular biomechanical properties in ovariectomized rats, indicating its potential efficacy in preventing osteoporotic fractures.

Key words: osteoporosis, ovariectomized rats, bone loss, Bushen Jianpi Huoxue Formula, biomechanics, trabecular biomechanical parameters

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