中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6127-6131.doi: 10.3969/j.issn.2095-4344.2012.33.010

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

抗阻训练衰老小鼠骨骼肌p53与bax基因的表达

王立丰1，李海鹏2，卢 健   

  1. 1华东师范大学体育与健康学院，上海市 200241；
    2 上海体育学院运动人体科学学院，上海市 200081
  • 收稿日期:2012-06-04 修回日期:2012-07-17 出版日期:2012-08-12 发布日期:2012-08-12
  • 作者简介:王立丰☆，男，1982年生，辽宁省朝阳市人，华东师范大学在读博士，主要从事运动与骨骼肌衰老的研究。 wanglifeng1982@126.com

Effect of resistance training on p53 and bax gene expression in the skeletal muscle of aging mice

Wang Li-feng1, Li Hai-peng2, Lu Jian1   

  1. 1Department of Physical Education and Health, East China Normal University, Shanghai 200241, China;
    2School of Human Sports Science, Shanghai University of Sport, Shanghai 200081, China
  • Received:2012-06-04 Revised:2012-07-17 Online:2012-08-12 Published:2012-08-12
  • About author:Wang Li-feng☆, Studying for doctorate, Department of Physical Education and Health, East China Normal University, Shanghai 200241, China wanglifeng1982@126.com

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摘要：

背景：抗阻训练能够缓解肌肉衰减征的发生并能够弱化骨骼肌细胞凋亡。
目的：观察年龄和抗阻训练对SAMP8小鼠骨骼肌p53，bax基因表达的影响。
方法：分别取3月龄(青年组)和6月龄(老年组)的SAMP8小鼠进行8周爬梯运动，每周3次，以不参加运动的同龄小鼠作为对照。
结果与结论：Real-time PCR结果显示，与青年组比较，老年组小鼠骨骼肌纤维p53，bax mRNA的表达量显著上升(P < 0.05)，而与相同月龄对照组比较，抗阻训练的小鼠骨骼肌纤维中p53，bax mRNA的表达量明显降低(P < 0.05)。提示抗阻训练能抑制衰老小鼠骨骼肌p53，bax基因的表达。

关键词: 肌肉衰减征, 细胞凋亡, 骨骼肌纤维, p53, bax, 抗阻训练

Abstract:

BACKGROUND: Resistance training can alleviate muscle attenuation and weaken the apoptosis of skeletal muscle cells.
OBJECTIVE: To observe the effects of aging and resistance training on the expression of p53 and bax genes in the skeletal muscle of SAMP-8 mice.
METHODS: SAMP-8 mice aged 3 months and 6 months were selected to undergo 8-week ladder movement (three times per week). Another mice at the same age without movement served as controls.
RESULTS AND CONCLUSION: Real-time PCR showed that p53 and bax mRNA expression increased significantly in the skeletal muscle fibers of 3-month-old mice than 6-month-old mice (P < 0.05). However, compared with those at the same age, p53 and bax mRNA expression in the skeletal muscle fibers decreased significantly in mice undergoing ladder movements (P < 0.05). These findings indicate that resistance movements can suppress p53 and bax gene expression in the skeletal muscle of aging mice.

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