中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6132-6136.doi: 10.3969/j.issn.2095-4344.2012.33.011

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

多囊卵巢综合征大鼠骨骼肌细胞胰岛素受体底物1及其丝氨酸307位点磷酸化的表达

安广丽，王 娈，马瑞欣   

  1. 青岛大学医学院附属医院内分泌科，山东省青岛市 266003
  • 收稿日期:2012-05-02 修回日期:2012-06-13 出版日期:2012-08-12 发布日期:2013-07-15
  • 通讯作者: 马瑞欣，博士，主任医师，教授，青岛大学医学院附属医院内分泌科，山东省青岛市 266003 ruixinma@yahoo.com
  • 作者简介:安广丽★，女，1986年生，山东省泰安市人，汉族，青岛大学医学院在读硕士，主要从事代谢综合征相关疾病的研究。 angul-0707@163.com

Expression of insulin receptor substrate 1 and its serine 307 site phosphorylation in rat skeletal muscle with polycystic ovary syndrome

An Guang-li, Wang Luan, Ma Rui-xin   

  1. Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
  • Received:2012-05-02 Revised:2012-06-13 Online:2012-08-12 Published:2013-07-15
  • Contact: Ma Rui-xin, Doctor, Chief physician, Professor, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China ruixinma@yahoo.com
  • About author:An Guang-li★, Studying for master’s degree, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China angul-0707@163.com

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摘要：

背景：胰岛素受体底物1的丝氨酸307位点磷酸化程度的增加参与了骨骼肌胰岛素抵抗的发生。
目的：观察多囊卵巢综合征大鼠骨骼肌细胞胰岛素受体底物1的含量及其丝氨酸307位点磷酸化程度的变化。
方法：将大鼠随机分为模型组和对照组，模型组给予人绒毛膜促性腺激素联合胰岛素皮下注射，并配以高脂饮食，构建大鼠多囊卵巢综合征模型；对照组皮下注射生理盐水，正常饲料喂养。
结果与结论：干预6周后，Western blot检测结果显示，与对照组相比，模型组大鼠骨骼肌细胞胰岛素受体底物1表达量显著下降(P < 0.05)，而其丝氨酸307位点磷酸化蛋白表达明显升高(P < 0.05)。结果提示，多囊卵巢综合征大鼠骨骼肌细胞中胰岛素受体底物1蛋白表达下调及其丝氨酸307位点磷酸化蛋白表达上调与多囊卵巢综合征大鼠骨骼肌胰岛素抵抗的发生密切相关。

关键词: 多囊卵巢综合征, 胰岛素抵抗, 胰岛素受体底物 1, 丝氨酸, 磷酸化, 骨骼肌, 大鼠, 组织工程

Abstract:

BACKGROUND: Increased serine 307 phosphorylation of insulin receptor substrate 1 is involved in the occurrence of insulin resistance in the skeletal muscle.
OBJECTIVE: To observe the expression of insulin receptor substrate 1 and its serine 307 phosphorylation in the skeletal muscle of polycystic ovary syndrome rats.
METHODS: Female Sprague-Dawley rats were randomized to model group and control group. Insulin and human chorionic gonadotrophin were administered to the model group rats which were fed with high fat diet and 50 g/L glucose solution. The rats in the control group were given normal diet and injected normal saline daily.
RESULTS AND CONCLUSION: Compared with the control group, expression of insulin receptor substrate 1 decreased significantly (P < 0.05), while its serine 307 phosphorylation increased obviously (P < 0.05) in the model group at 6 weeks after intervention. These findings suggest that reduction of insulin receptor substrate1 protein expression and increase of its serine 307 phosphorylation are closely related with the occurrence of skeletal muscle insulin resistance in rats with polycystic ovary syndrome.

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