中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 6037-6041.doi: 10.3969/j.issn.2095-4344.2012.32.028

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

诱导多能干细胞移植后对急性心肌梗死小鼠心律的影响

魏新伟1，张晓刚2，杨 梁3   

  1. 1安阳市人民医院心内一科，河南省安阳市 455000；
    2重庆医科大学附属第一医院心内科，重庆市 400016；
    3成都市第三人民医院内科ICU，四川省成都市 610000
  • 收稿日期:2012-02-21 修回日期:2012-02-21 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 张晓刚，博士，教授，主任医师，硕士生导师，重庆医科大学附属第一医院心内科，重庆市 400016 zxg0233@sina.com
  • 作者简介:魏新伟★，男，1972年生，河南省安阳市人，汉族，2011年重庆医科大学毕业，硕士，主治医师，主要从事细胞或组织心脏移植的电生理学研究。 wxwzs720504@163.com

Transplantation of induced pluripotent stem cells for cardiac rhythm in mice with acute myocardial infarction

Wei Xin-wei1, Zhang Xiao-gang2, Yang Liang3   

  1. 1First Department of Cardiology, People’s Hospital of Anyang, Anyang 455000, Henan Province, China;
    2Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
    3ICU of Department of Internal Medicine, Third People’s Hospital of Chengdu, Chengdu 610000, Sichuan Province, China
  • Received:2012-02-21 Revised:2012-02-21 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Xiao-gang, M.D., Professor, Chief physician, Master’s supervisor, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • About author:Wei Xin-wei★, Master, Attending physician, First Department of Cardiology, People’s Hospital of Anyang, Anyang 455000, Henan Province, China wxwzs720504@163.com

PDF

432

被引次数

4

摘要：

背景：诱导多能干细胞被认为是治疗缺血性心肌病最具前景的一种方法，但其移植的安全性、有效性仍需进一步研究。
目的：探讨诱导多能干细胞移植后对急性心肌梗死小鼠心脏节律产生的影响。
方法：建立ICR小鼠心肌梗死模型并将其随机分为急性心肌梗死组，急性心肌梗死+生理盐水组，急性心肌梗死+诱导多能干细胞组，急性心肌梗死+成纤维细胞组，同时设立正常对照组。各组分别于移植诱导多能干细胞5 min、1周、2周、3周后，应用BL-420生物机能系统检测各组小鼠体表心电图肢体Ⅱ导联心律的变化。免疫组化染色法检测各组小鼠心肌缝隙连接蛋白43的表达，并应用Image Proplus软件进行半定量分析。
结果与结论：与急性心肌梗死组和急性心肌梗死+成纤维细胞组比较，移植2，3周时急性心肌梗死+诱导多能干细胞组小鼠体表心电图Ⅱ导联室性早搏发生率明显减少，梗死心肌缝隙连接蛋白43表达明显增加(P < 0.05)，前两组相比差异无显著性意义。结果说明诱导多能干细胞移植2周后可明显减少梗死后小鼠室性早搏发生率，进而改善心肌组织的电活动并增强其电位稳定性，可使小鼠梗死心肌缝隙连接蛋白43的表达增加，而成纤维细胞移植的小鼠中则未出现此现象。

关键词: 诱导多能干细胞, 移植, 急性心肌梗死, 心律, 室性早博, 缝隙连接蛋白43

Abstract:

BACKGROUND: Induced pluripotent stem cells have been considered as a most promosing method for treatment of ischemic heart disease. However, the safety and efficiency of transplantation of induced pluripotent stem cells require further investigation.
OBJECTIVE: To investigate the effects of induced pluripotent stem cells transplantation on cardiac rhythm of mice with acute myocardial infarion.
METHODS: Acute myocardial infarction mouse models were established and were then randomly divided into acute myocardial infarction group, acute myocardial infarction + saline group, acute myocardial infarction + induced pluripotent stem cells group, and acute myocardial infarction + fibroblasts group. At the same time, a normal control group was designated. The change in the cardiac rhythm of the limb lead II surface electrocardiogram of every group was examined by BL-420 biological function system at 5 minutes, 1, 2, 3 weeks after transplantation of induced pluripotent stem cells. The expression of connexin 43 in each group was detected by immunohistochemical staining and semi-quantitatively analyzed using Image Proplus software.
RESULTS AND CONCLUSION: Compared with acute myocardial infarction and acute myocardial infarction + fibroblast groups, the ratio of ventricular premature in the acute myocardial infarction + induced pluripotent stem cells group was obviously shortened and connexin 43 expression was significantly increased (P < 0.05). There was no significant difference between acute myocardial infarction group and acute myocardial infarction + fibroblast group. At 2 weeks after transplantation of induced pluripotent stem cells, the ratio of ventricular premature was significantly decreased, thereby the electrical activity of myocardial tissue was improved and the potential stability was enhanced, which leads to increase in connexin 43 expression. However, this phenomenon was not observed in mice receiving transplantation of fibroblasts.

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