中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 5108-5112.doi: 10.3969/j.issn.2095-4344.2012.27.032

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

神经干细胞的鉴定

张俊虎，王建交，郑永日   

  1. 哈尔滨医科大学附属第二医院神经外科第二病房，黑龙江省哈尔滨市 150086
  • 收稿日期:2011-11-02 修回日期:2011-11-30 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 哈尔滨医科大学附属第二医院神经外科第二病房，黑龙江省哈尔滨市 150086
  • 作者简介:张俊虎★，男，1985年生，河南省信阳市人，汉族，哈尔滨医科大学在读硕士，主要从事神经干细胞移植修复神经系统损伤研究。 zjhgx6868@163.com

Identification of neural stem cells

Zhang Jun-hu, Wang Jian-jiao, Zheng Yong-ri   

  1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Received:2011-11-02 Revised:2011-11-30 Online:2012-07-01 Published:2013-11-01
  • Contact: Zheng Yong-ri, Chief physician, Master’s supervisor, Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Zhang Jun-hu★, Studying for master’s degree, Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China zjhgx6868@163.com

PDF

478

被引次数

15

摘要：

背景：神经干细胞的数量比较少，形态无特异性，又缺乏表面特异性抗原，因此对于神经干细胞的鉴定和纯化较难。
目的：综述神经干细胞鉴定的主要手段。
方法：由第一作者检索1990/2011 PubMed数据及万方数据库有关神经干细胞的特性、主要鉴定方法以及神经干细胞的应用前景等相关文献。
结果与结论：目前鉴定神经干细胞的方法主要有：①活体染料鉴定法。②表面标志物鉴定法：主要标志物有Nestin、vimentin、Musashi1、Musashi2、CD133(AC133)、唾液酸-神经细胞黏附分子、Lewis X LeX/SSEA-1、p75 神经营养受体、表皮生长因子受体等。③分子生物学鉴定方法。④核素标记法。表面标志物鉴定法及分子生物学鉴定法及将两种方法联合应用将是神经干细胞鉴定的主要手段，也是有可能取得突破性进展的研究手段。

关键词: 神经干细胞, 鉴定, 活体染料, 表面标志物, 分子生物学, 干细胞

Abstract:

BACKGROUND: Due to the less number of neural stem cells, no specific morphology and lack of surface antigen, the identification and purify of the neural stem cells are difficult.
OBJECTIVE: To summarize the main method to identify the neural stem cells.
METHODS: The PubMed database and Wanfang database form 1990 to 2011 were used to search the articles on the characteristics of neural stem cells, main identification method and application prospects.
RESULTS AND CONCLUSION: At present, the main method to identify the neural stem cells was in vivo dye identification method, surface marker identification method, molecular biology identification method and nuclide notation. The markers included Nestin, vimentin, Musashi1, Musashi2, CD133 (AC133), sialic acid-neural cell adhesion molecule, Lewis X LeX/SSEA-1, p75 neurotrophin receptor and epidermal growth factor. The surface marker identification method, molecular biology identification method and the combination of two methods were the main methods to identify the neural stem cells. It is a possible research tools to obtain breakthrough.

中图分类号: