中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 5034-5038.doi: 10.3969/j.issn.2095-4344.2012.27.018

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

表皮干细胞与毛囊干细胞生物学特性的比较

杨 青1，李秀兰2，张 杨2，郭 悦2，孙晓雷2   

  1. 1天津医科大学，天津市 300070；
    2天津医院骨科研究所，天津市 300211
  • 收稿日期:2012-04-06 修回日期:2012-05-02 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 李秀兰，教授，博士生导师，天津医院骨科研究所，天津市 300211 Lixiulan1954@sina.com
  • 作者简介:杨青★，女，1981年生，河北省邯郸市人，汉族，天津医科大学在读硕士，主要从事组织、细胞工程与组织损伤修复的研究。 qingyang567@126.com
  • 基金资助:

    天津市科技支撑计划重点项目(08ZCGYSF01300)；天津市自然科学基金项目(11JCYBJC10200)

Biological characteristics of epidermal stem cells and follicle stem cells

Yang Qing1, Li Xiu-lan2, Zhang Yang2, Guo Yue2, Sun Xiao-lei2   

  1. 1Tianjin Medical University, Tianjin 300070, China;
    2Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China
  • Received:2012-04-06 Revised:2012-05-02 Online:2012-07-01 Published:2013-11-01
  • Contact: Li Xiu-lan, Professor, Doctoral supervisor, Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China Lixiulan1954@sina.com

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摘要：

背景：获取更合适的组织工程皮肤种子细胞可使皮肤功能得到更好的修复。
目的：分离、培养表皮干细胞和毛囊干细胞，并比较两种细胞的生物学特性。
方法：体外分离培养2月龄新西兰兔表皮干细胞和毛囊干细胞，取生长良好的第2，3，6代细胞观察其生物学特性。
结果与结论：毛囊干细胞较表皮干细胞贴壁快，具有更高的增殖活性。免疫组化及荧光定量PCR检测显示毛囊干细胞β1整合素、角蛋白19蛋白及mRNA表达均明显高于表皮干细胞。提示作为皮肤组织工程的种子细胞，毛囊干细胞较表皮干细胞更具优势。

关键词: 表皮干细胞, 毛囊干细胞, 角蛋白19, β1整合素, 基因, 生物学特性, 种子细胞, 皮肤组织工程, 干细胞

Abstract:

BACKGROUND: More appropriate skin tissue engineering seed cells can better repair skin function.
OBEJECTIVE: To isolate and culture epidermal stem cells and follicle stem cells, and compare their biological characteristics.
METHODS: Epidermal stem cells and follicle stem cells of 2-month-old New Zealand rabbits were isolated and cultured in vitro. The biological characteristics of passage 2, 3, 6 cells which grew well were observed.
RESULTS AND CONCLUSIONS: Follicle stem cells attached faster and had higher proliferative capacity than epidermal stem cells. Immunohistochemical staining and fluorescent quantitative PCR showed that the follicle stem cells showed higher protein and mRNA expression of β1 integrin and K19 than epidermal stem cells. These findings suggest that follicle stem cells, as seed cells of skin tissue engineering, are superior to epidermal stem cells.

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