中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 4953-4958.doi: 10.3969/j.issn.2095-4344.2012.27.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨形态发生蛋白2体外诱导大鼠骨髓间充质干细胞向心肌样细胞的分化

高辰玮，王海萍，吴志刚，吕 洋，霍艳丽，王浩宇   

  1. 河北北方学院组织学与胚胎学教研室，河北省张家口市 075000
  • 收稿日期:2011-11-28 修回日期:2011-12-12 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 王海萍，博士，副教授，河北北方学院组织学与胚胎学教研室，河北省张家口市 075000 haipingmimi@126.com
  • 作者简介:高辰玮★，女，1985年生，河北省沧州市人，汉族，河北北方学院在读硕士，主要从事干细胞研究。 tianbiandiren@163.com
  • 基金资助:

    河北省自然基金(C071101)

Bone morphogenetic protein-2 induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro

Gao Chen-wei, Wang Hai-ping, Wu Zhi-gang, Lü Yang, Huo Yan-li, Wang Hao-yu   

  1. Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2011-11-28 Revised:2011-12-12 Online:2012-07-01 Published:2013-11-01
  • Contact: Wang Hai-ping, M.D., Associate professor, Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Hebei Province, China haipingmimi@126.com

PDF

345

被引次数

1

摘要：

背景：目前研究者尝试使用各种细胞因子、中药单体和组织裂解液等诱导剂作用于其分化过程，用以修复梗死的心肌组织。
目的：探索骨形态发生蛋白2在大鼠骨髓间充质干细胞体外诱导分化为心肌样细胞过程中的作用。
方法：分离培养SD大鼠骨髓间充质干细胞。应用含有骨形态发生蛋白2的培养基定向诱导培养(对照组为普通培养基培养) 72 h，其后换用普通培养基继续培养4周。
结果与结论：初分离的细胞经纯化培养后，显示CD29阳性、CD34阴性，符合骨髓间充质干细胞的特征，生长曲线显示各代细胞生长规律相近。经骨形态发生蛋白2体外诱导培养后，细胞形态狭长，排列紧密、方向一致。分化后的细胞均阳性表达C-TnI、C-TnT 、desmin、α-sarcomeric actin和P38MAPK。空白对照组则基本不表达以上蛋白。提示骨形态发生蛋白2可以诱导大鼠骨髓间充质干细胞体外定向分化为心肌样细胞，是骨髓间充质干细胞体外心肌分化的一种新的有效诱导剂。

关键词: 骨形态发生蛋白2, 心肌样细胞, 骨髓间充质干细胞, 细胞培养, 细胞分化, 干细胞

Abstract:

BACKGROUND: Many kinds of cytokines, traditional Chinese medicine monomers and tissue lysate have been used for the differentiation process by investigators. And they try to treat myocardial infarction.
OBJECTIVE: To explore the effect of bone morphogenetic protein-2 in the differentiation of rat bone marrow mesenchymal stem cells into cardiomyocyte-like cells in vitro.
METHODS: Bone marrow mesenchymal stem cells were isolated from SD rat bone marrow and cultured for 72 hours with the medium containing bone morphogenetic protein-2 (the control group was cultured with normal medium), and then cultured with normal medium for 4 weeks.
RESULTS AND CONCLUSION: After purification and culture, the cells showed CD29 positive and CD34 negative, which met the characteristics of bone marrow mesenchymal stem cells, the growth curve showed the cells had similar growtj patterns. Bone marrow mesenchymal stem cells exhibited a narrow morphology and tightly packed in the same direction after induced by bone morphogenetic protein-2. The differentiated cells were positive for C-TnI, C-TnT, desmin, α-sarcomeric actin and P38MAPK. There was no positive staining in control group. Bone morphogenetic protein-2 can induce the bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro. It is a new and effective inductor for bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro.

中图分类号: