中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (34): 5430-5435.doi: 10.3969/j.issn.2095-4344.1960

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

不同微结构磷酸三钙材料对Wnt非经典信号通路相关标志物蛋白的表达影响

陈凯瑞1，2，包崇云3，刘  显3，肖  宇3，谭艳林2，刘秀飘2，熊博凯2，张  强2
  

  1. 1广州市妇女儿童医疗中心口腔外科，广东省广州市  510000；2南昌大学第一附属医院口腔颌面外科，江西省南昌市  330006；3四川大学华西口腔医院口腔疾病国家重点实验室，四川省成都市 400030
  • 收稿日期:2019-06-27 出版日期:2019-12-08 发布日期:2019-12-08
  • 通讯作者: 张强，博士，教授，硕士生导师，南昌大学第一附属医院口腔颌面外科，江西省南昌市 330006
  • 作者简介:陈凯瑞，男，1990年生，硕士，主要从事颌面部先天畸形的研究。
  • 基金资助:

    国家自然科学基金(81560189)；项目负责人：张强

Effects of tricalcium phosphate materials with different microstructures on the expression of non-canonical Wnt signaling pathway-related markers

Chen Kairui1, 2, Bao Chongyun3, Liu Xian3, Xiao Yu3, Tan Yanlin2, Liu Xiupiao2, Xiong Bokai2, Zhang Qiang2 
  

  1. 1Department of Oral and Maxillofacial Surgery, Guangzhou Women and Children's Medical Center, Guangzhou 510000, Guangdong Province, China; 2Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; 3State Key Laboratory of Oral Diseases, West China School/Hospital of Stomatology, Sichuan University, Chengdu 400030, Sichuan Province, China
  • Received:2019-06-27 Online:2019-12-08 Published:2019-12-08
  • Contact: Zhang Qiang, MD, Professor, Master’s supervisor, Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Chen Kairui, Master, Department of Oral and Maxillofacial Surgery, Guangzhou Women and Children's Medical Center, Guangzhou 510000, Guangdong Province, China; Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Supported by:

    The National Natural Science Foundation of China, No. 81560189 (to ZQ)

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摘要：

文章快速阅读：

 

文题释义：
骨诱导：诱导间充质细胞向成骨谱系分化并在非成骨部位形成骨组织，主要包括间充质细胞的募集，间充质细胞的骨向分化和异位成骨发生。
Wnt非经典信号通路：指部分Wnt蛋白不依赖β-catenin而激活下游的信号通路，传统上非经典信号通路是相互覆盖及交联的复杂网络状信号通路，但是依据主要特点又可以近似的分为Wnt/PCP信号途径、Wnt-cGMP/ Ca2+信号途径、Wnt/ROR2信号途径及其他一些途径。
 
 
背景：骨诱导的发生机制目前还不明确，骨诱导过程中支架材料微结构通过调控相关骨向分化信号通路从而发挥关键性作用。
目的：探讨不同微结构磷酸三钙材料对MG63细胞中Wnt非经典信号通路相关标志物表达水平的影响。
方法：利用H2O2发泡及控制烧结温度合成具有不同微结构的磷酸三钙材料(TCP-compact，TCP-middle，TCP-big)，通过压汞实验、扫描电镜扫描确证材料微结构；同时将已确证的3组具有不同微结构磷酸三钙材料与MG63细胞共培养，检测Wnt非经典信号通路的标志物(Wnt5a，FZD1，ROR2，DKK1)的表达。
结果与结论：与具有微孔结构材料共培养的细胞中Wnt5a，FZD1，ROR2，DKK1表达水平显著提高(P < 0.05)，不同孔径的材料之间Wnt5a，ROR2，DKK1表达存在差异(P < 0.05)；在共培养末期材料组之间4组标记物表达无显著差异(P > 0.05)。材料微结构影响Wnt非经典信号通路的表达水平，其相关标志蛋白表达水平的差异为材料微孔结构所调控，在一定范围内材料孔径越大Wnt非经典信号通路被明显促进。

ORCID: 0000-0002-1737-1591(陈凯瑞)

关键词: 骨缺损, 骨诱导, 骨向分化, 磷酸三钙, 微结构, 孔隙率, 材料孔径, Wnt非经典信号通路, Wnt5a, ROR2

Abstract:

BACKGROUND: The mechanism of osteoinduction is still unclear. The scaffold material microstructure plays a key role in the process of osteoinduction by regulating the relevant bone differentiation signaling pathway.
OBJECTIVE: To investigate the effects of tricalcium phosphate with different microstructures on the expression levels of related markers in non-canonical Wnt signaling pathway in MG63 cells.
METHODS: Tricalcium phosphate materials with different microstructures (TCP-compact, TCP-middle, TCP-big) were synthesized by controlling H2O2 foaming and sintering temperature. The microstructure of the material was confirmed by mercury intrusion porosimetry and scanning electron microscopy. At the same time, three types of tricalcium phosphate materials with different microstructures were co-cultured with MG63 cells to detect the expression of non-canonical Wnt signaling pathway-related markers (Wnt5a, FZD1, ROR2, DKK1).
RESULTS AND CONCLUSION: The expression levels of Wnt5a, FZD1, ROR2 and DKK1 were significantly increased in MG63 cells co-cultured with microporous materials (P < 0.05). The expressions of Wnt5a, ROR2 and DKK1 were significantly different between materials with different pore sizes (P < 0.05). There was no significant difference in the expression of Wnt5a, FZD1, ROR2 and DKK1 between three material groups (P > 0.05). The material microstructure affected the expression level of non-canonical Wnt signaling pathway. The difference of the expression level of related markers was regulated by material microstructure. The larger the pore size of the material, the greater the Wnt non-canonical signaling pathway was promoted.

Key words: bone defect, osteoinduction, bone differentiation, tricalcium phosphate, microstructure, porosity, material pore size, non-canonical Wnt signaling pathway, Wnt5a, ROR2

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