中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (9): 1461-1467.doi: 10.3969/j.issn.2095-4344.1098

• 干细胞综述 stem cell review • 上一篇    下一篇

细胞自噬与肌腱病的靶向自噬途径

朱前拯1,高福强2,林 朋1,孙 伟2   

  1. 中日友好医院,1骨科三部,2骨科一部,北京市 100029
  • 修回日期:2018-10-07 出版日期:2019-03-28 发布日期:2019-03-28
  • 通讯作者: 孙伟,博士,教授,中日友好医院骨科一部,北京市 100029
  • 作者简介:朱前拯,1981年生,山西省太原市人,北京大学医学部毕业,博士,主要从事创伤与运动损伤的基础与临床研究。
  • 基金资助:

    国家自然科学基金面上项目(81672236,81372013);项目负责人:孙伟;北京市自然科学基金面上项目(7182146);项目负责人:孙伟;北京化工大学-中日友好医院联合基金项目(PYBZ1828);项目负责人:孙伟

Effects of autophagy on tendinopathy

Zhu Qianzheng1, Gao Fuqiang2, Lin Peng1, Sun Wei2   

  1. 1Third Department of Orthopedics, 2First Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China
  • Revised:2018-10-07 Online:2019-03-28 Published:2019-03-28
  • Contact: Sun Wei, MD, Professor, First Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China
  • About author:Zhu Qianzheng, MD, Third Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China
  • Supported by:

    the National Natural Science Foundation of China (General Program), No. 81672236 and 81372013 (to SW); the Natural Science Foundation of Beijing (General Program), No. 7182146 (to SW); the Beijing University of Chemical Technology & China-Japan Friendship Hospital Joint Foundation, No. PYBZ1828 (to SW)

摘要:

文章快速阅读:

文题释义:
细胞自噬:
自噬是真核细胞中在进化上高度保守的分解代谢现象,用于降解回收细胞内生物大分子和细胞器,
如错误折叠的蛋白质、衰老或受损的线粒体等,维持细胞的稳态和存活,被认为是Ⅱ型程序性细胞死亡。自噬可分为3种:大自噬、小自噬以及分子伴侣介导的自噬。
肌腱病:是临床常见的疾病类型,常见于运动损伤和职业损伤,慢性或急性牵张负荷可导致肌腱炎症、退变和损伤,临床表现为疼痛和活动受限,可进一步发展为运动失能或骨关节炎。

 

摘要
背景:
肌腱是一种连接骨骼和肌肉的纤维组织,主要功能是在运动时从肌肉向骨骼传导应力,肌腱病是临床常见的疾病类型,表现为肌腱炎症、退变和损伤。细胞自噬广泛参与许多疾病的发展。基于细胞自噬的研究方法为肌腱损伤的修复提供了新的思路。
目的:综述细胞自噬的发生过程和调控机制,分析细胞自噬参与肌腱病的病理机制,为肌腱病的预防治疗提供参考。
方法:应用计算机检索中国知网、万方数据库及PubMed 数据库关于自噬与肌腱病的相关研究的文章,中文检索词为“自噬、肌腱、成纤维细胞、肌腱病”,英文检索词为“autophagy,tendon,fibroblast,tendinopathy”。进行系统的归纳总结和分析,排除内容相关性差或重复文献,最终得到54篇文献。
结果与结论:自噬可以减轻氧化应激对人肌腱干细胞的损害;随着肌腱组织的细胞外基质降解程度的增加,肌腱细胞由于过度的细胞自噬出现自噬性细胞死亡;前列腺素E2以剂量依赖的方式显著诱导成纤维细胞的死亡和自噬;肩袖损伤后的肌肉萎缩受自噬调控;雷帕霉素通过自噬的激活抑制肌腱损伤后的腱周纤维化。综合目前研究结果,自噬在肌腱病中有重要作用,自噬将会成为肌腱病新的研究热点,更加详细地了解自噬过程及其在肌腱病中的作用,有助于研究人员找到肌腱病的靶向自噬途径,为干预和治疗肌腱病提供新的理论和方法支持。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:0000-0002-6916-7648(朱前拯)

关键词: 细胞自噬, 肌腱病, 肌腱细胞, 成纤维细胞, 细胞死亡, 肩袖, 雷帕霉素, 前列腺素E2, 细胞外基质

Abstract:

BACKGROUND: Tendon is a fibrous tissue that connects bone and muscle. The main function is to conduct stress from the muscles to the bone during exercise. Tendinopathy is a commonly seen disease, characterized by tendon inflammation, degeneration and injury. Autophagy is widely involved in the development of many degenerative diseases. The research method based on autophagy provides a new idea for tendon repair. 
OBJECTIVE: To review the process and regulation mechanism of autophagy, and to analyze the pathological mechanism of autophagy involved in the tendinopathy so as to provide a reference for the prevention and treatment of tendinopathy. 
METHODS: The articles concerning autophagy and tendinopathy were retrieved by computer in CNKI, WanFang and PubMed databases. The keywords were “autophagy, tendon, fibroblast, tendinopathy” in English and Chinese, respectively. Finally, 54 articles were obtained through systematic induction and analysis after excluding the irrelevant and repetitive articles.   
RESULTS AND CONCLUSION: Autophagy can alleviate the damage to human tendon stem cells induced by oxidative stress. With the increase of the degree of extracellular matrix degradation in the tendon tissue, autophagic cell death occurs in the tendon cells due to excessive autophagy. Prostaglandin E2 significantly induces fibroblast death and autophagy in a dose-dependent manner. The muscle atrophy after the rotator cuff injury is regulated by autophagy. Rapamycin prevents peritendinous fibrosis through activation of autophagy. In conclusion, autophagy plays an important role in tendinopathy. Autophagy will become a new hotspot in tendinopathy. Further understanding of autophagy and its role in tendinopathy will contribute to finding a targeted autophagy pathway and provide new theoretical and methodological support for the intervention and treatment of tendinopathy. 


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Autophagy, Tendons, Fibroblasts, Tissue Engineering

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