中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (10): 1488-1492.doi: 10.3969/j.issn.2095-4344.0706

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

同步辐射相衬CT对骨移植材料骨再生的成像

刘永超1，2，简建波1，李长春2，孙莲莲2，胡春红1
  

  1. 1天津医科大学生物医学工程与技术学院，天津市  300070；2天津市第五中心医院，天津市  300450
  • 收稿日期:2017-11-04 出版日期:2018-04-08 发布日期:2018-04-08
  • 通讯作者: 胡春红，教授，博士生导师，天津医科大学生物医学工程与技术学院，天津市 300070
  • 作者简介:刘永超，男，1979年生，河北省迁安市人，汉族，天津医科大学在读硕士，主管技师，主要从事同步辐射成像研究研究。
  • 基金资助:
    天津市滨海新区卫生计生委科技项目重点支持项目(2015BWKZ001)

Bone regeneration imaging after bone grafting using phase-contrast computed tomography with synchrotron radiation

Liu Yong-chao1, 2, Jian Jian-bo1, Li Chang-chun2, Sun Lian-lian2, Hu Chun-hong1
  

  1. 1School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China; 2Tianjin Fifth Central Hospital, Tianjin 300450, China
  • Received:2017-11-04 Online:2018-04-08 Published:2018-04-08
  • Contact: Hu Chun-hong, Professor, Doctoral supervisor, School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China
  • About author:Liu Yong-chao, Master candidate, Technologist-in-charge, School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China
  • Supported by:
    the Science and Technology Key Project of the Health and Family Planning Commission of Binhai New Region, Tianjin, China, No. 2015BWKZ001

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摘要：

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文题释义：
同步辐射相衬CT成像：作为一种全新的X射线成像技术，原则上能够分辨出密度变化在0.000 3-0.002 g/cm3之间不同组织的边界，图像空间分辨率也可以达到1 μm，在生物体内软组织和低原子序数材料成像领域得到了广泛应用。应用同步辐射光源相衬CT成像不会破坏到生物体内的器官脏器和组织结构，保证了组织结构的完整性。
同步辐射光源：是极其新型的辐射光源，它拥有常规光源不能与之比拟的多种优异特性，尤其是光源具有高亮度、高准直性及相干性，这些特性为X射线的单色化和发展多种相位衬度的成像方法提供了可能。
 
摘要
背景：不同于传统CT的吸收信息成像，同步辐射相衬CT成像以一种前所未有的成像机制解决了生物体内软组织的微观结构成像难题。
目的：采用同步辐射相衬CT成像技术进行移植骨材料骨再生的成像研究。
方法：在4只新西兰大白兔股骨干骺端制造缺损模型，随机分2组，分别在缺损中注入磷酸钙合成骨移植材料和Bio-Oss骨移植材料，移植2周后，采用同步辐射相衬CT扫描股骨干骺端缺损标本，扫描之后进行病理切片苏木精-伊红染色与天狼星红染色。
结果与结论：①Bio-Oss骨移植材料组：同步辐射相衬CT扫描显示，在距离骨移植材料远处可见并不依附骨移植材料而生长的网状支架结构；苏木精-伊红染色病理切片可见大量红染的类骨质，呈骨小梁样排列，周围有大量成骨细胞分布，成骨反应明显；天狼星红染色病理切片可见大量黄染，Ⅰ型胶原表达明显，未见绿色染色，同时可见圆形或椭圆形的成骨细胞Ⅰ型胶原包埋其中；②磷酸钙合成骨移植材料组：同步辐射相衬CT扫描并未观察到骨诱导形成的网状结构，仅能在移植材料周围观察到爬行状态的类骨质，依附在移植骨周围；苏木精-伊红染色病理切片可见大量红染的类骨质；天狼星染色病理切片可见红黄染；③结果表明：同步辐射相衬CT可清晰显示移植骨材料的骨再生微观结构。

关键词: 生物材料, 同步辐射, 骨移植材料, 骨再生, 类骨质, 三维重建, 骨缺损, 微观结构, 生物材料, 成骨细胞, 细胞外基质

Abstract:

BACKGROUND: With a clear distinction from traditional computed tomography (CT) imaging with information absorption, phase-contrast CT with synchronous radiation has implemented the microstructure imaging of soft  tissues in organisms with an unprecedented imaging mechanism.
OBJECTIVE: To explore the synchrotron radiation phase-contrast CT imaging technology in the bone regeneration imaging after bone grafting.
METHODS: Four New Zealand white rabbits were used to make a metaphyseal defect model. Then, model rabbits were randomized into a group with calcium phosphate bone grafting and a group with Bio-Oss bone grafting in the defects. The specimens were imaged by the synchrotron radiation phase-contrast CT and stained with hematoxylin-eosin and sirius red 2 weeks after bone grafting.
RESULTS AND CONCLUSION: (1) Bio-Oss bone graft material group: Osteoid was observed not only around the graft material but also in the area far from the graft bone material as reticulate structure by the synchrotron radiation phase-contrast CT. Hematoxylin-eosin staining showed a large amount of red osteoid tissues arranged as trabecular bone, and a large amount of osteoblasts with obvious osteogensis. Sirius red-stained pathological sections were largely stained yellow, and there were round or oval osteoblasts with strongly expressed type I collagen. (2) Calcium phosphate bone graft material group: There was no reticulate structure shown by the synchrotron radiation phase-contrast CT, and the creep of osteoid tissues was only around the bone graft. Hematoxylin-eosin staining showed a large amount of red osteoid tissues, and sirius red-stained pathological sections were stained yellow and red. To conclude, the synchronous radiation phase- contrast CT can clearly display the regenerated structure of bone grafts.

Key words: Bone Transplantation, Bone Regeneration, Tissue Engineering

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