中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (8): 1229-1234.doi: 10.3969/j.issn.2095-4344.0141

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

富氢水对心肌缺血再灌注模型大鼠心肌损伤的保护作用

王  赞1，刘  莉1，张  喆1，孙晓宇1，李翔子1，李志林2，刘福林3，周玉娟1   

  1. 河北大学，1医学院，2化学院，河北省保定市  071000； 3河北大学附属医院，河北省保定市  071000
  • 收稿日期:2017-10-07 出版日期:2018-03-18 发布日期:2018-03-18
  • 通讯作者: 周玉娟，硕士，教授，硕士生导师，河北大学医学院，河北省保定市 071000 并列通讯作者：刘福林，硕士，主任医师，教授，硕士生导师，河北大学附属医院，河北省保定市 071000
  • 作者简介:王赞，女，1991年生，河北省石家庄市人，汉族，河北大学在读硕士，主要从事药理学心血管方向研究。
  • 基金资助:

    河北省 2013年医学科学研究重点课题计划项目(20130369)

Hydrogen rich water protects against myocardial ischemia/reperfusion injury in rats

Wang Zan1, Liu Li1, Zhang Zhe1, Sun Xiao-yu1, Li Xiang-zi1, Li Zhi-lin2, Liu Fu-lin3, Zhou Yu-juan1   

  1. 1School of Medicine, 2School of Chemistry, Hebei University, Baoding 071000, Hebei Province, China; 3Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • Received:2017-10-07 Online:2018-03-18 Published:2018-03-18
  • Contact: Zhou Yu-juan, Master, Professor, Master’s supervisor, School of Medicine, Hebei University, Baoding 071000, Hebei Province, China Corresponding author: Liu Fu-lin, Master, Chief physician, Professor, Master’s supervisor, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • About author:Wang Zan, Master candidate, School of Medicine, Hebei University, Baoding 071000, Hebei Province, China
  • Supported by:

    the Key Medicine Research Program of Hebei Province in 2013, No. 20130369

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摘要：

文章快速阅读：

文题释义：
氢化酶体：氢化酶体(hydrogenosome)一种膜包裹的球状细胞器，双膜结构，其中含有氢化酶、丙酮酸铁氧还蛋白氧化还原酶、乙酸:琥珀酸辅酶A转移酶和琥珀酸连接酶、SOD、苹果酸脱氢酶、铁氧还蛋白，腺苷酸激酶和NADH:铁氧还蛋白等。氢化酶体可产生ATP、二氧化碳、乙酸和氢气。
氢水：氢气医学主要围绕氢气治疗疾病等生物学效应和机制的研究，氢气能通过选择性抗氧化发挥治疗疾病的作用，把氢气溶解在水中可以制作成氢水。氢气医学研究氢分子的医学效应，氢水是氢气分子溶解在水的溶液，不是氢离子溶液，也不是碱性水。氢分子医学所说的氢水只是负氢水，是富含负电子的氢水，是还原剂氢水。准确地说，是在微酸性水和弱酸性水中生成，即pH值为4-6.8之间生成1 000 ppm以上的富(负)氢水。
摘要
背景：近几年来，大量研究发现低浓度氢气或富氢水或氢气饱和生理盐水对多种疾病具有神奇的保护作用，包括心肌缺血再灌注损伤。
目的：探讨富氢水对心肌缺血再灌注损伤的保护作用。
方法：将48只Wistar大鼠随机分为对照组与富氢水组，2组再分别随机分成缺血前期、缺血期、缺血再灌注期，每期8只大鼠。取大鼠心脏，按逆灌注10 min，常温旷置20 min，再灌注20 min的方法建立心肌缺血再灌注模型。对照组采用预先用氧平衡(体积分数95%O2+5%CO2)的37 ℃ K-R液灌注，富氢水组采用预先用氧平衡(体积分数95%O2+5%CO2)的37 ℃ K-R液+富氢水灌注(0.6 mmol/L，pH 7.3)。实验完毕后取材，苏木精-伊红染色观察两组大鼠心肌组织的心肌病理学改变，测定心肌组织中乳酸脱氢酶、肌酸激酶的活性，双抗夹心ELISA法检测肿瘤坏死因子α、白细胞介素1β的水平。
结果与结论：①对照组缺血期与缺血再灌注期心肌乳酸脱氢酶活性均高于缺血前期(P < 0.05)；富氢水组各期心肌乳酸脱氢酶活性差异无显著性意义；与对照组相比富氢水组缺血再灌注期乳酸脱氢酶活性明显降低 (P < 0.05)；②对照组缺血再灌注期肌酸激酶活性均显著高于缺血前期和缺血期(P < 0.05)；富氢水组各期肌酸激酶活性差异无显著性意义；与对照组比较富氢水组缺血再灌注期肌酸激酶活性明显降低(P < 0.05)；③对照组和富氢水组缺血再灌注期肿瘤坏死因子α、白细胞介素1β均显著高于缺血期和缺血前期(P < 0.05)；缺血期高于缺血前期(P < 0.05)；与对照组相比富氢水组缺血再灌注期两因子水平明显降低(P < 0.05)，但仍高于缺血前期；④结果说明，富氢水对离体大鼠心脏心肌缺血再灌注损伤有保护作用，其机制可能是降低肿瘤坏死因子α、白细胞介素1β水平的表达，即减少了炎症反应。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-0811-8292(王赞)

关键词: 组织构建, 富氢水, 心肌, 缺血再灌注损伤

Abstract:

BACKGROUND: Increasing evidence has indicated that low-concentration hydrogen or hydrogen rich water or hydrogen saturated saline exerts a protective effect on various diseases, such as myocardial ischemia/reperfusion injury.
OBJECTIVE: To explore the protective effect of hydrogen rich water on myocardial ischemia/reperfusion injury.
METHODS: Forty-eight Wistar rats were equally randomized into control and hydrogen-rich groups, and then subdivided into ischemic preconditioning, ischemia, and ischemia/reperfusion groups (n=8 rats in each subgroup). The myocardial ischemia/reperfusion model was established in the heart of each rat by the following procedures: reverse perfusion for 10 minutes, room temperature for 20 minutes, and reperfusion for 20 minutes. The control rats was perfused with pre-oxygenated (95% O2 plus 5% CO2) 37 ℃ K-R solution and the hydrogen-rich group was perfused with pre-oxygen-equilibrated (95% O2 plus 5% CO2) 37 ℃ K-R solution plus hydrogen-rich water      (0.6 mmol/L, pH=7.3). Subsequently, the heart was removed, the pathological changes of the myocardial tissues were observe by hematoxylin-eosin staining, the activities of lactic dehydrogenase and creatine kinase in the myocardial tissues were determined, and the levels of tumor necrosis factor-α and interleukin-1β were detected by ELISA.
RESULTS AND CONCLUSION: In the control group, the activity of lactic dehydrogenase at the ischemic and ischemia/reperfusion stages was significantly higher than that at the ischemic preconditioning stage (P < 0.05), and the activity of creatine kinase at the ischemia/reperfusion stage was significantly higher than that at the ischemic preconditioning and ischemic stages (P < 0.05). In the hydrogen-rich group, there was no significant difference in the activities of lactic dehydrodenase and creatine kinase at each stage, but the activities of at the ischemia/reperfusion stage was significantly lower than those in the control group (P < 0.05). In the two groups, the order of the levels of tumor necrosis factor-α and interleukin-1β was as follows: the ischemia/reperfusion stage > ischemic stage > ischemic preconditioning stage (P < 0.05). The levels of above factors in the hydrogen-rich group were significantly lower than those in the control group (P < 0.05). Our findings imply that hydrogen rich water has protective effect on myocardial ischemia/reperfusion injury of the rat hearts in vitro, which may be by reducing the expression of tumor necrosis factor-α and interleukin-1β, and further alleviating the inflammatory response.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hydrogen, Myocardium, Reperfusion Injury, Creatine Kinase, Tissue Engineering

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