中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (4): 587-592.doi: 10.3969/j.issn.2095-4344.0095

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

新型玻璃化冻存技术在活肿瘤组织样本库建设中的应用

张  源,杨秋蕊,张洪丹,朱雪晶,李伟建,王振宇,景宏舒,翟  博   

  1. 上海交通大学医学院附属仁济医院肿瘤介入科,上海市  200127
  • 收稿日期:2017-12-16 出版日期:2018-02-08 发布日期:2018-02-08
  • 通讯作者: 翟博,博士,主任医师,教授,上海交通大学医学院附属仁济医院肿瘤介入科,上海市 200127
  • 作者简介:张源,男,1992年生,上海市人,汉族,上海交通大学医学院在读硕士,主要从事肝脏等实体良恶性肿瘤的临床与基础研究。
  • 基金资助:

    国家自然科学基金资助项目(81472845)

A novel vitrified cryopreservation method for the establishment of tumor tissue biobank

Zhang Yuan, Yang Qiu-rui, Zhang Hong-dan, Zhu Xue-jing, Li Wei-jian, Wang Zhen-yu, Jing Hong-shu, Zhai Bo   

  1. Department of Interventional Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine,  Shanghai 200127, China
  • Received:2017-12-16 Online:2018-02-08 Published:2018-02-08
  • Contact: Zhai Bo, M.D., Chief physician, Professor, Department of Interventional Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • About author:Zhang Yuan, Studying for master’s degree, Department of Interventional Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Supported by:

    The National Natural Science Foundation of China, No. 81472845

摘要:

文章快速阅读:

文题释义:
玻璃化冻存技术:活性组织与高浓度冻存液充分反应后快速降温,转变为玻璃样半透明状,并予以低温保存。组织在冻存过程中细胞内外无冰晶形成,避免了细胞的结构破坏,可高度保存组织活性。
活肿瘤穿刺组织样本库:按严格技术标准采集活性肿瘤穿刺组织,经玻璃化冻存处理后低温长期储存,同时匹配建立与之相关的临床治疗、病理、随访、知情同意等资料库及其质量控制、信息管理与应用系统。
摘要
背景:
玻璃化冻存是一种新型的低温保存技术,在临床活肿瘤组织样本库的建设中具有潜在的应用价值。
目的:探讨新型玻璃化冻存技术的有效性以及在活肿瘤穿刺组织样本库建设中的可行性。
方法:将新鲜直肠癌肝转移活检组织样本随机分为2组,实验组作玻璃化冻存处理,对照组不作处理。取部分复苏后活检组织和部分新鲜活检组织分别接种于小鼠背部皮下,建立人源化移植瘤模型。对比观察活检肿瘤组织冻存前后生物学活性和组织学特征的变化。Calcein-AM活细胞染色和Hoechst33342染色反映细胞活性变化,Ki67标记免疫组织化学及苏木精-伊红染色反映组织的增殖能力和形态学特征变化。初步收集105例直肠癌肝转移活检组织并作玻璃化冻存,建立活性肿瘤穿刺组织生物样本库。
结果与结论:直肠癌肝转移活检组织在玻璃化冻存前后未发生显著的生物学活性和形态学特征改变(P > 0.05),说明新型玻璃化冻存技术可有效冻存活性穿刺肿瘤组织,并对生物样本库的建设具有实际应用价值。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0003-0140-9935(张源)

关键词: 生物样本库, 低温冻存, 玻璃化冻存, 活性保存, 肝恶性肿瘤, 直肠癌肝转移, 组织构建, 活检穿刺组织, 国家自然科学基金

Abstract:

BACKGROUND: Vitrified cryopreservation is a novel method for specimen preservation, which has a potential application value in the establishment of tumor biopsy biobank.
OBJECTIVE: To investigate the effectiveness of vitrified cryopreservation and the feasibility in the establishment of tumor biopsy biobank.
METHODS: The fresh biopsy specimens of liver metastasis from rectal cancer were randomized into vitrified cryopreservation and control groups. Then, the biopsy specimens from each group were implanted subcutaneously into the mouse back to establish the humanized mouse xenograft model. The biological characteristics and histological changes of tumor tissues in each group were detected by Calcein-AM/Hoechst33342 staining, as well as immunohistochemistry and hematoxylin-eosin staining. Totally 105 biopsy tissues of liver metastasis from rectal cancer were preliminarily collected and cryopreserved to establish the tumor biopsy biobank.
RESULTS AND CONCLUSION: No significant differences in biological viability or histological features of tumor biopsy was detected before and after cryopreservation (P > 0.05), suggesting that vitrified cryopreservation can effectively preserve tumor biopsy and is favorable for the establishment of tumor biopsy biobank.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Cryopreservation, Liver Neoplasms, Tissue Engineering

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