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文题释义：
循环压力：椎间盘可因异常的力学作用而导致其发生退变，力学刺激中压力已被证明可导致椎间盘的合成代谢减弱，分解代谢增强，细胞发生退变，前期研究表明对小型动物兔脊柱施加0.1-0.3 MPa，每12-24 h加压1次，每次30-120 min可在不导致椎间盘组织坏死的情况下使终板软骨发生退变。
器官培养：将具有较高面积-容积比的小型动物兔的椎间盘完整取出置于含有青链双抗(青霉素50 U/mL和链霉素50 mg/L)、体积分数20%胎牛血清的DMEM/F12(1∶1)培养液的培养皿里，再放进37 ℃和体积分数5%CO2培养箱进行培养，既可克服在在体动物上加力的不稳定性，又可避免细胞培养中易发生活性丢失的问题。
.jpg)
文题释义：
循环压力：椎间盘可因异常的力学作用而导致其发生退变，力学刺激中压力已被证明可导致椎间盘的合成代谢减弱，分解代谢增强，细胞发生退变，前期研究表明对小型动物兔脊柱施加0.1-0.3 MPa，每12-24 h加压1次，每次30-120 min可在不导致椎间盘组织坏死的情况下使终板软骨发生退变。
器官培养：将具有较高面积-容积比的小型动物兔的椎间盘完整取出置于含有青链双抗(青霉素50 U/mL和链霉素50 mg/L)、体积分数20%胎牛血清的DMEM/F12(1∶1)培养液的培养皿里，再放进37 ℃和体积分数5%CO2培养箱进行培养，既可克服在在体动物上加力的不稳定性，又可避免细胞培养中易发生活性丢失的问题。