中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4682-4685.doi: 10.3969/j.issn.1673-8225.2012.25.025

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

脱细胞猪角膜表面基底膜成分的检测

林旭初1，金 岩2，惠延年3   

  1. 1解放军303医院眼科，广西壮族自治区南宁市 530021；
    2解放军第四军医大学口腔医院组织工程研究中心，陕西省西安市710032；
    3解放军第四军医大学西京医院眼科，陕西省西安市710032
  • 收稿日期:2011-11-12 修回日期:2011-12-01 出版日期:2012-06-17 发布日期:2013-11-04
  • 作者简介:林旭初☆，女，1968年生，安徽省桐城市人，汉族，2007年解放军第四军医大学毕业，博士，副主任医师，主要从事组织工程角膜及眼表疾病方面的研究。 linxuc2006@ 126.com
  • 基金资助:

    国家863基金资助(2005AA205241)

Detection of basement membrane components on the surface of acellular porcine cornea

Lin Xu-chu1, Jin Yan2, Hui Yan-nian3   

  1. 1Department of Ophthalmology, the 303 Hospital of Chinese PLA, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
    2Research Center of Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;
    3Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Received:2011-11-12 Revised:2011-12-01 Online:2012-06-17 Published:2013-11-04
  • About author:Lin Xu-chu☆, Doctor, Associate chief physician, Department of Ophthalmology, the 303 Hospital of Chinese PLA, Nanning 530021, Guangxi Zhuang Autonomous Region, China linxuc2006@126.com

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513

被引次数

2

摘要：

背景：前期实验显示脱细胞猪角膜具有良好的组织相容性，可以支持角膜细胞和皮肤上皮细胞的生长。
目的：检测脱细胞猪角膜是否保存了利于角膜上皮细胞生长的重要组织结构—基底膜。
方法：利用荧光抗体对脱细胞猪角膜表面的基底膜成分(层粘蛋白和Ⅳ型胶原)进行免疫组织化学检测，荧光显微镜下观察脱细胞猪角膜表面是否保存了基底膜成分。
结果与结论：免疫荧光染色显示脱细胞猪角膜前基质表面层粘蛋白和Ⅳ型胶原呈阳性表达，与新鲜猪角膜表面基底膜的荧光表达相同，表明脱细胞猪角膜保存了利于角膜上皮细胞生长的基底膜。

关键词: 脱细胞猪角膜, 基底膜, 层粘蛋白, Ⅳ型胶原, 荧光显微镜, 生物材料

Abstract:

BACKGROUND: The previous experiments have suggested that the acellular porcine cornea stroma (APCS) has a good compatibility and can support the growth of keratocytes and skin epithelial cells.
OBJECTIVE: To detect whether the important structure for the growth of corneal epithelial cells-basement membrane can be preserved on the surface of the APCS.
METHODS: Fluorescent antibody was used to detect the basement membrane component (laminin and collagen Ⅳ) by immunohistochemistry. Fluorescence microscopy was observed whether the basement membrane could be preserved on the surface of the APCS.
RESULTS AND CONCLUSION: Immunofluorescence staining showed that laminin and collagen Ⅳ positively expressed on the surfaces of porcine corneal stroma with the same level of fresh normal porcine corneal stroma. The results indicate the APCS can preserve the natural basement membrane component of the cornea which is beneficial to the growth of corneal epithelial cells.

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