中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4677-4681.doi: 10.3969/j.issn.1673-8225.2012.25.024

• 药物控释材料 drug delivery materials • 上一篇    下一篇

丝素蛋白/壳聚糖三维支架与骨髓间充质干细胞的体外培养

邓 江，佘荣峰，黄文良，苑 成，莫 刚   

  1. 遵义医学院第三附属医院骨科，贵州省遵义市 563002
  • 收稿日期:2011-10-09 修回日期:2011-11-09 出版日期:2012-06-17 发布日期:2013-11-04
  • 作者简介:邓 江，男，1963年生，四川省成都市人，汉族，1985年遵义医学院毕业，主任医师，硕士生导师，主要从事骨与关节创伤的研究。 DJ30666@ 126.com
  • 基金资助:

    贵州省科学技术基金项目(黔科合J字[2009]2172号)，课题名称：SF-CS复合诱导后骨髓间充质干细胞修复兔膝关节软骨缺损的研究

In vitro culture of bone marrow mesenchymal stem cells on silk fibroin/chitsan three-dimensional scaffolds

Deng Jiang, She Rong-feng, Huang Wen-liang, Yuan Cheng, Mo Gang   

  1. Department of Orthopedics, Third Affiliated Hospital, Zunyi Medical College, Zunyi 563002, Guizhou Province, China
  • Received:2011-10-09 Revised:2011-11-09 Online:2012-06-17 Published:2013-11-04
  • About author:Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopedics, Third Affiliated Hospital, Zunyi Medical College, Zunyi 563002, Guizhou Province, China DJ30666@126.com

PDF

473

被引次数

4

摘要：

背景：前期实验发现丝素蛋白、壳聚糖以适当的比例混合，可以互相弥补各自的不足，表现出良好的理化性质和生物学特性。
目的：观察骨髓间充质干细胞在丝素蛋白/壳聚糖混合三维支架材料上的生长情况。
方法：将诱导后的兔骨髓间充质干细胞接种在丝素蛋白/壳聚糖支架材料上，检测细胞黏附率，倒置显微镜及扫描电镜观察细胞生长情况。
结果与结论：细胞黏附率随时间的延长而增加。倒置显微镜观察显示，丝素蛋白/壳聚糖支架上的细胞看不清，随着时间的延长，支架周围细胞增多，且有细胞伸入支架内；扫面电镜观察显示，细胞生长活跃、增殖分裂正常，细胞周围见颗粒状、丝状基质物质，细胞的微丝与支架材料黏附紧密；细胞不仅可以在材料表面贴附生长，并伸入材料之中。说明丝素蛋白/壳聚糖混合支架材料具有良好的细胞生物相容性。

关键词: 丝素蛋白, 壳聚糖, 支架, 骨髓间充质干细胞, 生物材料, 组织工程

Abstract:

BACKGROUND: Preliminary experiments have confirmed that mixture of silk fibroin and chitosan in a proper ratio can make up their shortcomings to show the good physical and chemical properties and biological characteristics.
OBJECTIVE: To observe the growth of bone marrow mesenchymal stem cells (BMSCs) on silk fibroin/chitsan (SF/CS) composite three-dimensional scaffolds.
METHODS: Rabbit BMSCs after induction were seeded on SF/CS scaffolds. Cell adhesion rate was detected, and cell growth was observed under inverted microscope and scanning electron microscopy.
RESULTS AND CONCLUSION: The cell adhesion rate was increased as time went on. Inverted microscope: BMSCs on the scaffolds could not be seen clearly, the number of cells around the scaffolds was increased as time went on, and the cells extended into the scaffolds. Scanning electron microscopy: the cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds; the cells could not only show the attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. It is indicated that the SF/CS composite scaffolds have a good cell biocompatibility.

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