中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4447-4452.doi: 10.3969/j.issn.1673-8225.2012.24.016

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

生物素多聚葡聚糖胺追踪显示皮质脊髓束的走行和分布

巴迎春1，唐建中2，范 艳1，李仲铭1，王金德1，王廷华3   

  1. 昆明医学院，1人体解剖学教研室，3神经科学研究所(省部级重点实验室)，云南省昆明市 650500；
    2云南省第一人民医院肝胆科，云南省昆明市 650032
  • 收稿日期:2012-04-13 修回日期:2012-04-30 出版日期:2012-06-10 发布日期:2013-11-05
  • 通讯作者: 王廷华，博士，教授，昆明医学院神经科学研究所，云南省昆明市650500 tinghua_neuron@263.net
  • 作者简介:巴迎春☆，男，1971年生，云南省麻栗坡县人，汉族，2010年昆明医学院毕业，博士，副教授，主要从事脊髓损伤和胚胎脊髓发育的研究。 byc71206@yahoo.com.cn
  • 基金资助:

    云南省应用基础研究面上项目(2007COO48R)

Demonstration empirical study of the corticospinal tracts by biotinylated dextran amine tracer

Ba Ying-chun1, Tang Jian-zhong2, Fan Yan1, Li Zhong-ming1, Wang Jin-de1, Wang Ting-hua3   

  1. 1Department of Human Anatomy, 2Institute of Neuroscience, Kunming Medical College, Kunming 650500, Yunnan Province, China;
    3Department of Hepatology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Received:2012-04-13 Revised:2012-04-30 Online:2012-06-10 Published:2013-11-05
  • Contact: Wang Ting-hua, Doctor, Professor, Department of Hepatology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China tinghua_neuron@263.net
  • About author:Ba Ying-chun☆, Doctor, Associate professor, Department of Human Anatomy, Kunming Medical College, Kunming 650500, Yunnan Province, China byc71206@yahoo.com.cn

PDF

744

被引次数

4

摘要：

背景：生物素多聚葡聚糖胺的作用机制及代谢过程目前还不十分清楚，可能和与生物素连接的其他复合物相似。
目的：选择最佳的生物素多聚葡聚糖胺追踪(示踪)实验方法，使生物素多聚葡聚糖胺能充分显示皮质脊髓束的走行和分布。
方法：将15只SD雌性大鼠用于生物素多聚葡聚糖胺条件实验，分为双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5 μL/位点存活2周组、单侧(右侧)大脑皮质注射10%生物素多聚葡聚糖胺1 μL/位点存活3周组及双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5 μL/位点存活3周组，每组5只。
结果与结论：在3组进行生物素多聚葡聚糖胺追踪实验的大鼠中，双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5 μL/位点存活3周组的追踪效果最好。提示在双侧大脑皮质上选择多点注射，以每个位点注射0.5 μL的10%生物素多聚葡聚糖胺，注射后大鼠存活3周为最佳的生物素多聚葡聚糖胺追踪实验方案。

关键词: 生物素多聚葡聚糖胺, 追踪, 大鼠, 大脑皮质, 皮质脊髓束

Abstract:

BACKGROUND: Biotinylated dextran amine mechanisms and metabolic processes are not entirely clear, which may be similar to the complex linked with biotin.
OBJECTIVE: To choose the best biotinylated dextran amine tracing empirical method to fully show courser and distribution of the corticospinal tracts
METHODS: Fifteen normal Sprague-Dawley female rats were used in this study and divided into three groups, with five rats in each group: in the first group, rats survived for 2 weeks after 10% biotinylated dextran amine (0.5 μL/site) was injected into the bilateral cerebral cortices; while 10% biotinylated dextran amine (1 μL/site) was injected into the right cerebral cortex in the second group rats which survived for 3 weeks; in the third group, 10% biotinylated dextran amine (0.5 μL/site) was injected into the bilateral cerebral cortices, and the rats survived for 3 weeks.
RESULTS AND CONCLUSION: The trace effect of biotinylated dextran amine in the third group was the best. This indicates that the tracing effect of biotinylated dextran amine is optimal in the rats receiving multi-point injection of biotinylated dextran amine (10%, 0.5 μL/site) into the bilateral cerebral cortices and surviving for 3 weeks after injection.

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