中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4237-4241.doi: 10.3969/j.issn.1673-8225.2012.23.012

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

基质细胞衍生因子1及其受体CXCR4在内源性神经干细胞迁移中的作用

戴孝森，丁 鹏，董 岳，牟临杰，王伟民，王崇谦，王进昆   

  1. 昆明医学院第一附属医院神经外科，云南省昆明市 650032
  • 收稿日期:2011-10-06 修回日期:2011-11-30 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 王进昆，主任医师，硕士生导师，昆明医学院第一附属医院神经外科，云南省昆明市 650032
  • 作者简介:戴孝森★，男，1984年生，福建省三明市人，汉族，昆明医学院第一附属医院神经外科在读硕士，主要从事颅内肿瘤及脑血管病的治疗与研究。 Daixiaosen123456@qq.com 并列第一作者：丁鹏☆，男，1976年生，山东省淄博市人，汉族，2005年昆明医学院毕业，博士，副教授，主要从事干细胞研究及神经外科方面的研究。 pengdwfmc@163.com
  • 基金资助:

    2008年云南应用基础研究面上项目(2008ZC132M)。项目名称：趋化因子及其受体在神经干细胞迁移中的作用的研究

Effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of endogenous neural stem cells

Dai Xiao-sen, Ding Peng, Dong Yue, Mu Lin-jie, Wang Wei-min, Wang Chong-qian, Wang Jin-kun   

  1. Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Received:2011-10-06 Revised:2011-11-30 Online:2012-06-03 Published:2013-11-06
  • Contact: Wang Jin-kun, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Dai Xiao-sen★, Studying for master’s degree, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China Daixiaosen123456@qq.com

PDF

391

被引次数

5

摘要：

背景：神经干细胞具有体外、体外迁移的特性，但关于其迁移的具体机制还不十分清楚。
目的：探讨基质细胞衍生因子1及其受体CXCR4在神经干细胞体内迁移中的作用。
方法：制备脑皮质微量注射脂多糖脑损伤大鼠模型，于建模后3，5，7 d灌注取脑，采用冰冻切片免疫组织化学方法，动态监测基质细胞衍生因子1和nestin表达的时相变化。
结果与结论：免疫组织化学染色观察显示：基质细胞衍生因子1随时间推移表达的量逐渐增多。Nestin阳性细胞有明显向外延伸和迁移的迹象，尖端指向脂多糖注射区。结果可见基质细胞衍生因子1趋化体内表达其特异性受体的内源性神经干细胞向脂多糖注射区迁移。

关键词: 神经干细胞, 基质细胞衍生因子1, Nestin, 迁移, 脂多糖

Abstract:

BACKGROUND: Neural stem cells own the characteristics of migration in vitro. However, the mechanisms underlying the migration of these cells remain unclear.
OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of neural stem cells in vivo.
METHODS: The brain injured rat model was established with cortical micro-injection of lipopolysaccharide. At 3, 5, and 7 days after modeling, the brain tissue was harvested. Immunohistochemical staining of frozen section was used to detect the expression of stromal cell-derived factor-1 and nestin-positive cells in different times and phages.
RESULTS AND CONCLUSION: Immunohistochemical staining showed that the expression of stromal cell-derived factor-1 was in a time-dependent manner. Nestin-positive cells extended and migrated outward significantly and pointed to the lipopolysaccharide injection area. Stromal cell-derived factor-1 can induce the endogenous neural stem cells to move towards lipopolysaccharide injection area in vivo.

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