中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3828-3832.doi: 10.3969/j.issn.1673-8225.2012.21.007

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

聚甲基丙烯酸甲酯骨水泥对原代培养脊柱转移瘤细胞的影响和意义★

方  济，胡侦明，郝  杰，甘  强，王大武，何  斌，税  威，钟小明，张晓军，江  维，沈皆亮，杜  培，王志芳   

  1. 重庆医科大学附属第一医院骨外科，重庆市    400016
  • 收稿日期:2012-01-11 修回日期:2012-02-14 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 胡侦明，博士，主任医师，重庆医科大学附属第一医院骨外科，重庆市 400016 zhenminghu62@yahoo.com.cn
  • 作者简介:方济★，男，1986年生，江西省景德镇市人，汉族，重庆医科大学在读硕士，主要从事从事骨外科专业研究。jefffangji@gmail.com

Effect and significance of polymethylmethacrylate bone cement in primary spinal metastasis cells 

Fang Ji, Hu Zhen-ming, Hao Jie, Gan Qiang, Wang Da-wu, He Bin, Shui Wei, Zhong Xiao-ming, Zhang Xiao-jun, Jiang Wei, Shen Jie-liang, Du Pei, Wang Zhi-fang
 
  

  1. Department of Orthopedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Received:2012-01-11 Revised:2012-02-14 Online:2012-05-20 Published:2012-05-20
  • Contact: Hu Zhen-ming, Doctor, Chief physician, Department of Orthopedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China zhenminghu62@yahoo.com.cn
  • About author:Fang Ji★, Studying for master’s degree, Department of Orthopedics, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China jefffangji@gmail.com

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摘要：

背景：目前经皮椎体成形技术已广泛应用于治疗老年骨质疏松椎体压缩性骨折以及椎体肿瘤并取得良好疗效，但仍有一些问题需关注和探讨。
目的：观察聚甲基丙烯酸甲酯骨水泥对脊柱转移瘤细胞周期变化和细胞凋亡的影响，分析其对脊柱转移瘤的体外抗肿瘤效应。
方法：原代培养脊柱转移瘤细胞，将其分为对照组、拉丝期聚甲基丙烯酸甲酯骨水泥组、冷却后聚甲基丙烯酸甲酯骨水泥组，药物处理24 h后利用流式细胞术观察凋亡率以及细胞周期，通过SYBR GreenⅠ荧光实时定量-聚合酶链反应法检测肿瘤细胞Bax和Caspase-3 mRNA表达的变化。
结果与结论：聚甲基丙烯酸甲酯骨水泥有促进脊柱转移瘤细胞凋亡的作用，对照组、拉丝期聚甲基丙烯酸甲酯骨水泥组、冷却后聚甲基丙烯酸甲酯骨水泥组的凋亡率分别为(3.80±1.09)%，(43.46±8.55)%，(28.77±9.39)%，两骨水泥组均能明显提高细胞凋亡率，拉丝期聚甲基丙烯酸甲酯骨水泥组作用更明显(P < 0.05)。两骨水泥组均能明显减少G0/G1期细胞，增加S期细胞(P < 0.05)。拉丝期聚甲基丙烯酸甲酯骨水泥组作用于脊柱转移瘤细胞后Bax、Caspase-3 mRNA的表达显著高于对照组(P < 0.05)。提示聚甲基丙烯酸甲酯骨水泥可在体外细胞水平诱导肿瘤细胞凋亡，拉丝期聚甲基丙烯酸甲酯骨水泥作用更加显著。
 

关键词: 聚甲基丙烯酸甲酯, 脊柱转移瘤, 骨水泥, 细胞凋亡, 细胞周期, 实时定量-聚合酶链反应

Abstract:

BACKGROUND: Percutaneous vertebroplasty (PVP) has been widely used and achieved good results in the treatment of senile osteoporotic vertebral compression fracture and vertebral tumor. However, some issues still need further study.
OBJECTIVE: To evaluate the effects of polymethylmethacrylate bone cement (PMMA) on the apoptosis and cell cycles of spinal metastasis cells, and to investigate the possible mechanisms of PMMA acting on spinal metastases in vitro.
METHODS: After primary culture, spinal metastasis cells were divided into three groups: control group, drawing-off stage PMMA group, cool stage PMMA group. After 24-hour treatment, the apoptosis of spinal metastases cells and its relationship with cell cycles were determined by flow cytometry. The expression of Bax and Caspase-3 mRNA were observed by SYBR green Ⅰ fluorescence quantitative PCR method.
RESULTS AND CONCLUSION: The cell apoptosis rates of the control, drawing-off stage PMMA and cool stage PMMA groups were (3.80±1.09)%, (43.46±8.55)%, (28.77±9.39)%, respectively. The latter two groups had significant effect (P < 0.05). Both groups of PMMA remarkably reduced the number of cells in the G0/G1 phase, and increased that in the S phase (P < 0.05). Drawing-off stage PMMA group could markedly increase the mRNA expression of Caspase-3 and Bax as compared with the control group after treatment for 24 hours (P < 0.05). PMMA can significantly induce the apoptosis of spinal metastasis cells and arrest their cell cycles in the S phase in vitro, as well as, with even more significant effect in drawing-off stage than in cool stage.

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