中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2898-2900.doi: 10.3969/j.issn.1673-8225.2012.16.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

超快效液相色谱与液面法测定榄香烯微乳的含量及包封率****☆

刘星言1，刘  宏1，周广林2，展晓日2，刘  珺2，曾昭武1，丁元林1   

  1. 1广东医学院，广东省东莞市    523808；2杭州师范大学，浙江省杭州市  310012
  • 收稿日期:2012-01-04 修回日期:2012-02-24 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 丁元林，博士，正教授，广东医学院，广东省东莞市 523808 artgreenking@ 126.com
  • 作者简介:刘星言☆，女，1977年生，湖南省湘潭市人，汉族，2009年中南大学湘雅医学院毕业，博士，副研究员，主要从事纳米医药研究工作。lxy0428@ 126.com
  • 基金资助:

    国家青年自然基金项目(81001647)；广东省自然科学基金项目(9151051501000035)；浙江省科技厅资助项目(2009C33005)；中国博士后科学基金面上项目(20100471757)。

Drug content and entrapment efficiency of elemene microemulsion detected by ultra-fast liquid chromatography and liquid surface method

Liu Xing-yan1, Liu Hong1, Zhou Guang-lin2, Zhan Xiao-ri2, Liu Jun2, Zeng Zhao-wu1, Ding Yuan-lin1   

  1. 1Guangdong Medical College, Dongguan  523808, Guangdong Province, China; 2Hangzhou Normal University, Hangzhou  310012, Zhejiang Province, China
  • Received:2012-01-04 Revised:2012-02-24 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Ding Yuan-lin, Doctor, Professor, Guangdong Medical College, Dongguan 523808, Guangdong Province, China artgreenking@ 126.com
  • About author:Liu Xing-yan☆, Doctor, Associate researcher, Guangdong Medical College, Dongguan 523808, Guangdong Province, China lxy0428@126.com
  • Supported by:

    the National Youth Science Foundation of China, No. 81001647*; the Natural Science Foundation of Guangdong Province, No. 9151051501000035*; the Foundation of the Science and Technology Bureau of Zhejiang Province, No. 2009C33005*; the General Program of China Postdoctoral Science Foundation, No. 20100471757*

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摘要：

背景：超快速液相色谱法具有快速、准确的优点，可以用来检测榄香烯微乳的含量。榄香烯微乳的包封率目前尚没有较好检测方法。
目的：采用超快效液相色谱与液面法测定榄香烯微乳的含量及包封率。
方法：应用超快速液相色谱法检测榄香烯微乳含量，根据榄香烯不溶于水而浮于水面的原理，设计液面法检测榄香烯微乳的包封率。
结果与结论：色谱条件下β-榄香烯与辅料分离良好，β-榄香烯在3.94~27.58 mg/L范围内线性关系良好。榄香烯微乳中β-榄香烯平均含量为(8.273±0.018) g/L，平均包封率为(99.81±0.24)%。提示超快速液相色谱和液面法可用于检测榄香烯微乳的含量和包封率，简单、快速且准确，榄香烯微乳包封率好。

关键词:

背景：超快速液相色谱法具有快速、准确的优点, 可以用来检测榄香烯微乳的含量。榄香烯微乳的包封率目前尚没有较好检测方法。
目的：采用超快效液相色谱与液面法测定榄香烯微乳的含量及包封率。
方法：应用超快速液相色谱法检测榄香烯微乳含量, 根据榄香烯不溶于水而浮于水面的原理, 设计液面法检测榄香烯微乳的包封率。
结果与结论：色谱条件下&beta, -榄香烯与辅料分离良好, &beta, -榄香烯在3.94~27.58 mg/L范围内线性关系良好。榄香烯微乳中&beta, -榄香烯平均含量为(8.273±, 0.018) g/L, 平均包封率为(99.81±, 0.24)%。提示超快速液相色谱和液面法可用于检测榄香烯微乳的含量和包封率, 简单、快速且准确, 榄香烯微乳包封率好。

Abstract:

BACKGROUND: Ultra-fast liquid chromatography is fast and accurate to detect content of elemene microemulsion.
OBJECTIVE: To detect the drug content and entrapment efficiency of elemene microemulsion using ultra-fast liquid chromatography and liquid surface method.
METHODS: The drug content of elemene microemulsion was determined by ultra-fast liquid chromatography. As elemene was insoluble in water and floated on water, liquid surface method was designed to detect the encapsulation efficiency of elemene emulsion.
RESULTS AND CONCLUSION: It was better for β-elemene to separate from accessories under the chromatographic conditions, and β-elemene at a dose of 3.94-27.58 mg/L had a good linear relationship. The elemene microemulsion was characterized as (8.273 ± 0.018) g/L in content of β-elemene, and (99.81±0.24)% in average entrapment efficiency. The present study demonstrates that ultra-fast liquid chromatography and liquid surface method are simple, fast and accurate for determining the drug content and entrapment efficiency of elemene microemulsion. The elemene microemulsion has high entrapment efficiency.

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