中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2724-2727.doi: 10.3969/j.issn.1673-8225.2012.15.014

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

股动脉周围骨骼肌多点注射携带肝细胞生长因子基因重组质粒糖尿病大鼠肢体缺血骨骼肌的组织学变化*★◆

胡朝昶1, 2，哈小琴2，何玉梅3，谢富强4，邢占奎4，韩军平5   

  1. 1兰州大学口腔医学院，甘肃省兰州市  730000；       2解放军兰州军区总医院医学实验中心，甘肃省干细胞与基因药物重点实验室，甘肃省兰州市  730050；3白银市中西医结合医院，甘肃省白银市730900；4兰州大学第二附属医院口腔颌面外科，甘肃省兰州市 730010；5兰州大学病理研究所，甘肃省兰州市  730000
  • 收稿日期:2011-10-08 修回日期:2011-11-11 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 谢富强，教授，硕士生导师，兰州大学第二附属医院口腔颌面外科，甘肃省兰州市 730010 Xiefq@lzu.edu.cn 并列通讯作者：哈小琴，博士，主任医师，硕士生导师，解放军兰州军区总医院医学实验中心,甘肃省干细胞与基因药物重点实验室；甘肃省兰州市 730050 haxq@yahoo.com
  • 作者简介:胡朝昶★，男，1982年生，甘肃省白银市人，汉族，兰州大学口腔医学院在读硕士，主要从事口腔颌面外科方面工作。 hcc0602@yahoo.cn
  • 基金资助:

    甘肃省科技支撑计划(1011FKCA103)。

     

Changes in ischemic skeletal muscle of the limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors into the skeletal muscles around the femoral arteries

Hu Chao-chang1, 2, Ha Xiao-qin2, He Yu-mei3, Xie Fu-qiang4, Xing Zhan-kui4, Han Jun-ping5   

  1. 1School of Stomatology, Lanzhou University, Lanzhou  730000, Gansu Province, China; 2Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou  730050, Gansu Province, China; 3Baiyin Hospital of Integrated Traditional Chinese and Western Medicine, Baiyin  730900, Gansu Province, China; 4Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou  730010, Gansu Province, China; 5Institute of Pathology, Lanzhou University, Lanzhou  730000, Gansu Province, China
  • Received:2011-10-08 Revised:2011-11-11 Online:2012-04-08 Published:2012-04-08
  • Contact: Xie Fu-qiang, Professor, Master’s supervisor, Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730010, Gansu Province, China Xiefq@lzu.edu.cn Ha Xiao-qin, Doctor, Chief physician, Master’s supervisor, Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China haxq@yahoo.com
  • About author:Hu Chao-chang★, Studying for master’s degree, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China hcc0602@yahoo.cn
  • Supported by:

    Gansu Provincial Technology and Research Project, No. 1011FKCA103*

PDF

295

摘要：

背景：质粒载体因其较好的生物安全性和较高的体内维持时间，被广泛应用于基因治疗研究领域。
目的：观察携带肝细胞生长因子基因重组质粒pUDKH在糖尿病后肢缺血模型大鼠股动脉周围骨骼肌多点注射15 d后骨骼肌组织的病理学变化。
方法：利用STZ制备SD大鼠糖尿病模型。随机分为3组，建模后24 h内高浓度组注射pUDKH 200 μg/只，低浓度组注射pUDKH 100 μg/只，对照组注射等体积医用注射用水。15 d后，取大鼠的骨骼肌组织进行病理学观察。
结果与结论：对照组肌浆萎缩变性，纤维化增生并透明变性，浆细胞浸润变性，肌间隙变宽，有局灶性脓肿形成；pUDKH治疗后有丰富的微血管形成，高浓度组肌纤维横纹较低浓度组保持完整。提示携载肝细胞生长因子基因质粒pUDKH对糖尿病大鼠肢端缺血有治疗作用。
关键词：肢体缺血；pUDKH；糖尿病；基因治疗；疾病模型，动物
缩略语注释：HGF: hepatocyte growth factor，肝细胞生长因子；VEGF: vascular endothelial growth factor，血管内皮生长因子
doi:10.3969/j.issn.1673-8225.2012.15.014

关键词: 肢体缺血, pUDKH, 糖尿病, 基因治疗, 疾病模型, 动物

Abstract:

BACKGROUND: At present, plasmid vectors are widely used in gene therapy based on their better bio-security and a longer duration in vivo.
OBJECTIVE: To investigate the histological changes of ischemic skeletal muscle of the hind limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors (pUDKH) into muscles around the femoral arteries for 15 days.
METHODS: Streptozotocin was used to establish diabetes model in Sprague-Dawley rats, and then the rats were divided into three groups randomly. After 24 hours of modeling, rats in the high dosage group were injected with pUDKH at 200 μg for each, those in the low dosage group were injected with pUDKH at 100 μg for each, and those in the control group were treated with medical water for injection at the same volume. Skeletal muscles of the rats were removed for pathological observation at 15 days.
RESULTS AND CONCLUSION: Sarcoplasmic atrophy and degeneration, fibrotic hyperplasia and hyalinization, invasive degeneration of plasma cells, widened muscle space, and focal abscess could be seen in the control group. Plentiful capillaries were formed after the treatment by pUDKH, and stripes of muscle fibers were well kept in the high dosage group compared with the low dosage group. It is indicated that pUDKH carrying hepatocyte growth factors has the therapeutic effects on Limb ischemia of diabetic rats.
Hu CC, Ha XQ, He YM, Xie FQ, Xing ZK, Han JP. Changes in ischemic skeletal muscle of the limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors into the skeletal muscles around the femoral arteries. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2724-2727.     [http://www.crter.cn  http://en.zglckf.com]

中图分类号: