中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2715-2719.doi: 10.3969/j.issn.1673-8225.2012.15.012

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

大鼠背部脊柱两侧全层皮肤圆形创面增生性瘢痕愈合过程中臭氧的作用**☆

欧陕兴1，李小荣1，张  莉1，俞志坚2   

  1. 1解放军广州军区广州总医院放射科，广东省广州市   510010；2南方医科大学附属珠江医院影像科，广东省广州市   510282
  • 收稿日期:2011-10-01 修回日期:2011-12-27 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 李小荣，硕士，主治医师，解放军广州军区广州总医院放射科，广东省广州市 510010 lxrong2008@163.com
  • 作者简介:欧陕兴☆，男，1955年生，湖南省临武县人，2004年解放军第二军医大学毕业，博士，博士生导师，教授，主任医师，主要从事心胸疑难疾病影像诊断与非血管介入研究。oushanxing@sina.com
  • 基金资助:

    全军医学科研“十一五”B类课题项目(2006MB162)；广东省中医药局基金项目(20060124)

Effect of ozone on hypertrophic scar during the healing of bilateral circular full-thickness skin wounds on the back of rats

Ou Shan-xing1, Li Xiao-rong1, Zhang Li1, Yu Zhi-jian2   

  1. 1Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510282, Guangdong Province, China; 2Department of Image, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-10-01 Revised:2011-12-27 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Li Xiao-rong, Master, Attending physician, Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510282, Guangdong Province, China lxrong2008@163.com
  • About author:Ou Shan-xing☆, Doctor, Doctoral supervisor, Professor, Chief physician, Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510282, Guangdong Province, China oushanxing@sina.com
  • Supported by:

    Type B Subject Project of the Military Medical Science and Research Plan during “Eleventh Five-Year”, No.2006MB162*; Foundation Program of Traditional Chinese Medicine Department of Guangdong Province, No.20060124*

PDF

333

被引次数

10

摘要：

背景：预防硬膜外瘢痕的形成，可减少腰椎手术失败综合征的发生。臭氧应用于腰椎间盘突出症的治疗已取得较好的临床效果。
目的：观察臭氧对大鼠伤口瘢痕愈合过程的影响。
方法：外科切口法制备大鼠背部脊柱两侧圆形全层皮肤创面增生性瘢痕模型，分为3组，纯氧组及臭氧组分别注射纯氧及30~40 mg/L臭氧，总量均为5 mL，空白组不注射任何药物，每周2次，4周后取瘢痕/肉芽组织标本，利用苏木精-伊红染色及免疫组化方法，对比观察各组标本瘢痕/肉芽组织外观及肿瘤坏死因子α、碱性成纤维细胞生长因子表达的变化。
结果与结论：与空白组及纯氧组相比，臭氧组愈后瘢痕面积较小，上皮生成较多，炎性细胞浸润较少，胶原纤维较纤细，且断裂较多；臭氧组肿瘤坏死因子α阳性细胞数较低(P < 0.01)，碱性成纤维细胞生长因子阳性细胞数较高(P < 0.01)。提示臭氧通过其抗炎作用，减少炎性细胞浸润，并可通过抑制成纤维细胞及巨噬细胞释放的肿瘤坏死因子α以及提高碱性成纤维细胞生长因子的表达，减少胶原的过度合成，从而起到抑制肉芽组织炎症及瘢痕组织增生的作用。
关键词：臭氧；瘢痕；伤口愈合；肿瘤坏死因子α；碱性成纤维细胞生长因子
缩略语注释：TNF-α: tumor necrosis fact-alpha，肿瘤坏死因子α；bFGF: basic fibroblast growth factor，碱性成纤维细胞生长因子
doi:10.3969/j.issn.1673-8225.2012.15.012

关键词: 臭氧, 瘢痕, 伤口愈合, 肿瘤坏死因子&alpha, 碱性成纤维细胞生长因子

Abstract:

BACKGROUND: The prevention of epidural scar formation can reduce the incidence of failed back surgery syndromes. Ozone has been applied in the treatment of lumbar disc herniation, and has achieved good effect.
OBJECTIVE: To investigate the effect of ozone on the healing of wound and hypertrophic scar in rats.
METHODS: Hypertrophic scar models of bilateral circular full-thickness skin wounds were made on the back of rats using surgical incision method, and then the rats were divided into three groups: blank control group with no treatment, oxygen group treated with 5 mL pure oxygen and ozone group treated with 5 mL of 30-40 mg/L ozone. Rats in the latter two groups were injected twice a week; After 4 weeks, hypertrophic scar/granulation tissue samples were extracted. The appearance of hypertrophic scar/granulation tissue as well as the expression of tumor necrosis factor-α and basic fibroblast growth factor in each group were detected by hematoxylin-eosin staining immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the blank control and oxygen groups, the area of hypertrophic scar in the ozone group was smaller after wound healing, besides, there were more epithelia generated and less inflammatory cell infiltration, as well as thinner collagen fibers and more breakages. Furthermore, the expression of tumor necrosis factor α was significantly decreased (P < 0.01), while the expression of basic fibroblast growth factor was significantly increased (P < 0.01). These findings suggest that ozone can reduce inflammatory cell infiltration via its anti-inflammatory effect and decrease the oversynthesis of collagens through inhibiting the expression of tumor necrosis factor α and increasing the expression of basic fibroblast growth factor to inhibit the inflammation of granulation tissue and scar proliferation.
Ou SX, Li XR, Zhang L, Yu ZJ. Effect of ozone on hypertrophic scar during the healing of bilateral circular full-thickness skin wounds on the back of rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2715-2719.    
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