中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2686-2690.doi: 10.3969/j.issn.1673-8225.2012.15.006

• 骨组织构建 bone tissue construction • 上一篇    下一篇

中药补肾活骨方对去势骨质疏松大鼠骨代谢的影响★

何飞宇1, 2，沈  霖1，梅  亮2，帅  波1   

  1. 1华中科技大学同济医学院附属协和医院中西医结合骨伤科，湖北省武汉市  430022；2武汉市第七医院骨科，湖北省武汉市  430071
  • 收稿日期:2011-09-20 修回日期:2011-09-28 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 沈霖，博士生导师，华中科技大学同济医学院附属协和医院中西医结合骨伤科，湖北省武汉市 430022
  • 作者简介:何飞宇★，男，1971年生，湖北省武汉市人，汉族，硕士，主治医师，主要从事骨科方面的研究。

Effects of Bushen Huogu decoction on bone metabolism of ovariectomized osteoporotic rats★

He Fei-yu1, 2, Shen Lin1, Mei Liang2, Shuai Bo1   

  1. Effects of Bushen Huogu decoction on bone metabolism of ovariectomized osteoporotic rats★
  • Received:2011-09-20 Revised:2011-09-28 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Shen Lin, Doctoral supervisor, Department of Integrative Medicine and Trauma, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
  • About author:He Fei-yu★, Master, Attending physician, Department of Integrative Medicine and Trauma, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Orthopedics, the Seventh Hospital of Wuhan City, Wuhan 430071, Hubei Province, China hfyff71@gmail.com

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摘要：

背景：中药补肾活骨方可有效防治骨质疏松症，但其具体的药理学机制仍不是很清楚。25-羟基维生素D3和1，25-二羟基维生素D3是调节骨吸收与骨形成的重要的偶联因子。
目的：观察补肾中药对去势骨质疏松大鼠骨密度、骨生物力学、血清及肝肾组织中25-羟基维生素D3和1，25-二羟基维生素D3水平的影响。
方法：健康雌性SD大鼠108只随机等分为假手术组、模型组和治疗组。后2组摘除双侧卵巢，导致雌激素缺失，从而诱导骨质疏松症模型。治疗组大鼠造模后以中药补肾活骨方2 mL灌胃，2次/d。
结果与结论：与模型组相比，治疗组股骨头骨密度明显提高(P < 0.05)，最大应力和最大负荷指数明显增强(P < 0.05)，血液、肝脏和肾脏组织中25-羟基维生素D3和1，25 二羟基维生素D3水平明显提高(P < 0.05)；且接近于假手术组(P < 0.05)。提示补肾中药在雌激素缺失早期即可在分子水平上调节25-羟基维生素D3和1，25-二羟基维生素D3的表达水平，激活骨代谢提高骨密度增强骨质量达到预防骨质疏松的作用。
关键词：补肾活骨方；1，25-二羟基维生素D3；骨密度；骨生物力学；骨质疏松症；骨代谢
doi:10.3969/j.issn.1673-8225.2012.15.006
中图分类号: R318  文献标识码: A  

关键词: 补肾活骨方, 1, 25-二羟基维生素D3, 骨密度, 骨生物力学, 骨质疏松症, 骨代谢

Abstract:

BACKGROUND: Bushen Huogu decoction can effectively prevent and treat osteoporosis, but the concrete mechanism of pharmacology is still not clear. 25-hydroxy vitamin D3 and 1, 25-dihydroxyvitamin D3 are important coupling factors, which can regulate bone resorption and formation.
OBJECTIVE: To investigate the curative effects of Bushen Huogu decoction on the bone mineral density, bone biomechanics, and level of 25-hydroxy vitamin D3 and 1, 25-dihydroxyvitamin D3 in blood serum, liver and kidney in the ovariectomized osteoporotic rats.
METHODS: Totally 108 healthy female Sprague-Dawley rats were randomly divided into model group, sham-operated group, and treatment group. All rats had been ovariectomized to induce estrogen absence and further establish osteoporotic models, except those in sham-operated group. Treatment group of rats were intragastrically administrated with 2 mL Bushen Huogu decoction, twice a day.
RESULTS AND CONCLUSION: Compared with model group, the bone mineral density in the rat femur was significantly increased in the treatment group (P < 0.05), the index of maximal stress and maximal loading of the femoral head were also increased (P < 0.05). The concentration of 25-hydroxy vitamin D3 and 1, 25-dihydroxyvitamin D3 in blood serum, liver and kidney were significant higher in the treatment group than those in the model group, and the levels were similar with those in sham-operated group (P > 0.05). In the early period of estrogenic hormone absence, the Chinese kidney-tonifying drugs could activate bone metabolism, raise bone mineral density and reinforce quality of bone through up-regulating expression of 25-hydroxy vitamin D3 and 1, 25-dihydroxyvitamin D3.

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