中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2555-2558.doi: 10.3969/j.issn.1673-8225.2012.14.017

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

脐血间充质干细胞的适宜培养条件☆

张  涛1，李  敏2，徐佩茹2   

  1. 1新疆医科大学  第二附属医院泌尿外科，新疆维吾尔自治区乌鲁木齐市  830063；  2新疆医科大学第一附属医院儿科，新疆维吾尔自治区乌鲁木齐市 830054
  • 收稿日期:2011-10-02 修回日期:2011-11-14 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 徐佩茹，主任医师，教授，博士生导师，新疆医科大学第一附属医院儿科，新疆维吾尔自治区乌鲁木齐市 830054 xupeiru126@126.com
  • 作者简介:张涛☆，男，1975年生，陕西省宁强县人，汉族，新疆医科大学在读博士，主治医师，主要从事干细胞和泌尿系结石方面的研究。

Appropriate conditions for culture of umbilical cord blood mesenchymal stem cells  

Zhang Tao1, Li Min2, Xu Pei-ru2   

  1. 1Department of Urology, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China; 2Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-10-02 Revised:2011-11-14 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Xu Pei-ru, Chief physician, Professor, Doctoral supervisor, Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China xupeiru126@126.com
  • About author:Zhang Tao☆, Studying for doctorate, Attending physician, Department of Urology, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

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摘要：

背景：目前有关脐血间充质干细胞培养的实验较多，主要从改善培养基成分、脐血细胞的分离方法以及首次换液时间等方面进行调整，目前尚未建立一个安全、高效的培养体系。
目的：探讨脐血间充质干细胞的适宜培养条件。
方法：于新疆医科大学第一附属医院产科取健康产妇的脐血，比较不同换液时间、不同培养基、不同体积分数的胎牛血清对脐血间充质干细胞原代培养效果的影响。
结果与结论：首次第5~7天换液、低糖DMEM、IMDM培养基有利于脐血间充质干细胞的生长。但还不能认为含体积分数为30%胎牛血清与含体积分数为20%胎牛血清的培养基在培养脐血间充质干细胞方面有差别。
关键词：脐血/胎血；间充质干细胞；培养；培养基；换液时间；胎牛血清
缩略语注释：MSCs：mesenchymal stem cells，间充质干细胞
doi:10.3969/j.issn.1673-8225.2012.14.017

关键词: 脐血/胎血, 间充质干细胞, 培养, 培养基, 换液时间, 胎牛血清

Abstract:

BACKGROUND: There have been many experiments describing culture of umbilical cord blood mesenchymal stem cells (UCBMSCs) in terms of improvement of medium compositions, separation method of cord blood cells, and the time for changing liquid for the first time. A safe and efficient training system has not been established yet.
OBJECTIVE: To explore a better method for culture of UCBMSCs in vitro with different conditions.
METHODS: The umbilical cord blood of healthy parturiens was taken from the Department of Obstetrics of the First Affiliated Hospital of Xinjiang Medical University. Several factors which influence the primary culture process of UCBMSCs were analyzed include the time for changing liquid for the first time, different culture mediums and different levels of fetal bovine serum.
RESULTS AND CONCLUSION: Changing liquid at 5-7 days for the first time, low glucose DMEM or IMDE medium would be helpful to the growth of UCBMSCs. 30% fetal bovine serum and 20% fetal bovine serum did not produce different effects in culture of UCBMSCs.

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