中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2095-2098.doi: 10.3969/j.issn.1673-8225.2012.12.002

• 生物材料学术探讨 biomaterial academic discussion • 上一篇    下一篇

胶原改良聚乳酸电纺丝支架用于组织工程化输尿管的体外构建***★

许永德，符伟军，史建国，谭海颂，张  旭   

  1. 解放军总医院泌尿外科，北京市  100853
  • 收稿日期:2012-01-04 修回日期:2012-01-25 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 符伟军，博士，副教授，硕士生导师，解放军总医院泌尿外科，北京市 100853 fuweijun@ hotmail.com
  • 作者简介:许永德★，男，1985年生，山东省淄博市人，解放军军医进修学院在读硕士，医师，主要从事输尿管组织工程修复的研究。 xuyongd@ hotmail.com
  • 基金资助:

    国家自然科学基金(81070555)，北京市自然科学基金(2092029)和军队临床高新技术重点项目(413DG63J)。  

A modified electrospun polylactic acid/collagen scaffold for ureteral tissue engineering reconstruction in vitro 

Xu Yong-de, Fu Wei-jun, Shi Jian-guo, Tan Hai-song, Zhang Xu   

  1. Department of Urology Surgery, Chinese PLA General Hospital, Beijing  100853, China
  • Received:2012-01-04 Revised:2012-01-25 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Fu Wei-jun, Doctor, Associate professor, Master’s supervisor, Department of Urology Surgery, Chinese PLA General Hospital, Beijing 100853, China fuweijun@ hotmail.com
  • About author:Xu Yong-de★, Studying for master’s degree, Physician, Department of Urology Surgery, Chinese PLA General Hospital, Beijing 100853, China xuyongd@ hotmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81070555*; the Natural Science Foundation of Beijing, No. 2092029*; the Major Project of Clinical High and New Technology of Army Hospital, No.413DG23J*

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摘要：

背景：聚乳酸是一种应用广泛的细胞支架材料，但其疏水性和缺乏细胞识别信号影响了在组织工程器官构建中的应用。
目的：探讨Ⅰ型胶原蛋白改良聚乳酸电纺丝支架体外构建组织工程化输尿管的可行性。
方法：用Ⅰ型胶原蛋白醋酸溶液冻干法处理聚乳酸电纺丝，使Ⅰ型胶原蛋白吸附于电纺丝纤维表面，制成胶原改良电纺丝支架。将分离培养的输尿管上皮细胞分别接种于改良聚乳酸电纺丝支架和未处理的聚乳酸电纺丝支架上。
结果与结论：MTT检测显示输尿管上皮细胞在改良支架中生长良好，细胞整体活性在各时间点均明显优于未处理的聚乳酸电纺丝支架上的细胞。扫描电镜观察发现细胞在改良支架表面黏附良好，接种后5 d，支架表面大部分已被增殖的输尿管上皮细胞覆盖。说明胶原改良聚乳酸电纺丝支架能明显提高种子细胞的黏附和增殖活性，可用于体外构建组织工程化输尿管。
关键词：输尿管；电纺丝；聚乳酸；胶原；黏附；增殖；组织工程
doi:10.3969/j.issn.1673-8225.2012.12.002

关键词: 输尿管, 电纺丝, 聚乳酸, 胶原, 黏附, 增殖, 组织工程

Abstract:

BACKGROUND: Polylactic acid is a widely used scaffold material, but its hydrophobicity and lack of cell recognition signal limit its application.
OBJECTIVE: To investigate the feasibility of constructing a tissue engineered ureter using a modified polylactic acid/typeⅠ collagen compound scaffold in vitro. 
METHODS: Electrospinning polylactic acid naofibrous scaffolds were dipped into a acetic acid solution of type-Ⅰ collagen to make the collagen absorbed onto the nanofiber surface. Then ureter epithelial cells were seeded separately onto the modified electrospun polylactic acid naofibrous scaffolds or control scaffolds.
RESULTS AND CONCLUSION: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results confirmed that there was higher cellular activity in the collagen modified compound scaffolds, which was superior to cells untreated electrospinning polylactic acid scaffold at each time point. Scanning electron microscope showed that the most part of the surface of the modified scaffold was covered with ureter epithelial cells at 5 days after seeding. The modified polylactic acid/type collagen compound scaffold can obviously increase adhesion, growth and proliferation of the seeded cells, and has potential applications in ureteral tissue engineering reconstruction.

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