中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1765-1768.doi: 10.3969/j.issn.1673-8225.2012.10.013

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐带间充质干细胞影响子宫内膜异位症细胞的增殖及凋亡**☆

徐丽南1，徐冰南2，陈淑琴1，何  科1，蔡  坚1   

  1. 1中山大学附属第一医院妇产科，广东省广州市 510080；2佛山市第一人民医院妇产科，广东省佛山市  528000
  • 收稿日期:2012-01-16 修回日期:2012-01-29 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 徐丽南，博士，主治医师，中山大学附属第一医院妇产科，广东省广州市510080
  • 作者简介:徐丽南☆，女，1977年生，河南省安阳市人，汉族，2011年中山大学毕业，博士，主治医师，主要从事围产医学的临床与基础研究。drxulinan@gmail.com
  • 基金资助:

    国家自然科学基金项目(81070472)；广东省科技计划项目(2010B031600313)。

Human umbilical cord mesenchymal stem cells influence the proliferation and apoptosis of endometrial cells 

Xu Li-nan1, Xu Bing-nan2, Chen Shu-qin1, He Ke1, Cai Jian1   

  1. 1Department of Gynaecology and Obstetrics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China;    2Department of Gynaecology and Obstetrics, First People’s Hospital of Foshan, Foshan   528000, Guangdong Province, China
  • Received:2012-01-16 Revised:2012-01-29 Online:2012-03-04 Published:2012-03-04
  • About author:Xu Li-nan☆, M.D., Attending physician, Department of Gynaecology and Obstetrics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China drxulinan@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No.81070472*; Science and Technology Development Program of Guangdong Province, No.2010B031600313*

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摘要：

背景：人脐带间充质干细胞可通过旁分泌机制调控细胞的生物学活性。
目的：观察人脐带间充质干细胞对子宫内膜异位症细胞增殖及凋亡的影响。
方法：体外原代培养人异位子宫内膜细胞与人脐带间充质干细胞，实验组将脐带间充质干细胞和异位子宫内膜细胞按1×105/1×105比例接种于Transwell共培养板上下室，建立上下双层细胞非接触共培养体系，设1×105单独培养异位子宫内膜细胞为对照组。
结果与结论：①MTT法检测异位子宫内膜细胞增殖：与对照组比较，实验组细胞增殖明显受到抑制(P < 0.05)，且具有时间依赖性(P < 0.05)。②流式细胞仪检测异位子宫内膜细胞增殖凋亡：实验组凋亡细胞明显多于对照组(P < 0.05)，异位子宫内膜细胞由G1期进入S期细胞减少。③RT-PCR法检测异位子宫内膜细胞张力蛋白同源物基因mRNA的表达：实验组明显高于对照组。表明人脐带间充质干细胞可以通过旁分泌机制抑制人异位子宫内膜细胞的体外增殖并促进其凋亡，且可能是通过提高张力蛋白同源物基因基因表达来达到的。
关键词：人脐带间充质干细胞；子宫内膜异位症细胞；细胞凋亡；共培养；张力蛋白同源物基因
doi:10.3969/j.issn.1673-8225.2012.10.013

关键词: 人脐带间充质干细胞, 子宫内膜异位症细胞, 细胞凋亡, 共培养, 张力蛋白同源物基因

Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) can regulate cellular biological activities by paracrine mechanism.
OBJECTIVE: To investigate the effects of hUCMSCs on the proliferation and apoptosis of endometrial cells.
METHODS: Endometrial cells and hUCMSCs were cultured in vitro. hUCMSCs and endometrial cells were co-cultured on the Transwell co-culture plate at a proportion of 1×105/1×105 to establish upper and lower double-cell non-contact co-culture system, serving as the experimental group. Endometrial cells (1×105 cells/well) were separately cultured as control group.
RESULTS AND CONCLUSION: MTT assay results showed that cell proliferation in the experimental group was significantly inhibited in a time-dependent manner compared with the control group (P < 0.05). Flow cytometry results showed that apoptotic cells were significantly more in the experimental group than in the control group (P < 0.05), and endometrial cells entering into S stage from G1 stage were reduced. RT-PCR results showed that PTEN mRNA expression was significantly higher in the experimental group than in the control group. These findings suggest that hUCMSCs inhibit the in vitro proliferation of human endometrial cells and promote their apoptosis by paracrine mechanism, which occurs possibly by upregulation of PTEN mRNA expression.

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