中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1451-1454.doi: 10.3969/j.issn.1673-8225.2012.08.027

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

外源性单磷酸鸟苷环二聚体对变形链球菌基因表达的影响*☆

闫文娟，徐树军，谢苗苗，吴补领   

  1. 南方医科大学南方医院，南方医科大学口腔医学院，广东省广州市 510515
  • 收稿日期:2011-10-22 修回日期:2011-12-16 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 吴补领，主任医师，院长，主任，博士生导师，南方医科大学南方医院，南方医科大学口腔医学院，广东省广州市 510515 Wubuling@ yahoo.com.cn
  • 作者简介:闫文娟☆，女，1973年生，山东省潍坊市人，汉族，2009年解放军第四军医大学毕业，博士，讲师，主要从事龋病病因学研究。 ywj918@ sohu.com
  • 基金资助:

    国家自然科学基金支持项目(81100747)。

Effects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of streptococcus mutans 

Yan Wen-juan, Xu Shu-jun, Xie Miao-miao, Wu Bu-ling   

  1. Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-10-22 Revised:2011-12-16 Online:2012-02-19 Published:2012-02-19
  • Contact: Wu Bu-ling, Chief physician, Doctoral supervisor, Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China Wubuling@ yahoo.com.cn
  • About author:Yan Wen-juan☆, Doctor, Lecturer, Nanfang Hospital, School of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China ywj918@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No.81100747*

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摘要：

背景：前期研究证实变形链球菌内部存在单磷酸鸟苷环二聚体信号通路，该通路介导变形链球菌的生物膜形成及在体外牙釉质表面的黏附。
目的：以基因芯片技术分析单磷酸鸟苷环二聚体对变形链球菌生物学特性的影响。
方法：以外源性单磷酸鸟苷环二聚体干预变形链球菌UA159，提取其总RNA，并以标准菌株的总RNA作为对照，与变形链球菌全基因组芯片杂交，筛选差异基因。
结果与结论：外源性单磷酸鸟苷环二聚体干预后，变形链球菌中glgA、glgB、msmF、gftA、lacG、lepB、rgpAc、bacC 、apt69个基因表达上调，Hrc、ProC、HprT、Pdp、Glk、cdsA共6个基因表达下调。所获得的差异基因主要与细胞趋向性和生物膜形成、信号转导、糖代谢、等途径相关。说明单磷酸鸟苷环二聚体影响变形链球菌的致龋性。
关键词：基因芯片；单磷酸鸟苷环二聚体(c-di-GMP)；信号通路；变形链球菌；生物膜；致龋性
doi:10.3969/j.issn.1673-8225.2012.08.027

关键词: 基因芯片, 单磷酸鸟苷环二聚体(c-di-GMP), 信号通路, 变形链球菌, 生物膜, 致龋性

Abstract:

BACKGROUND: The preliminary study has confirmed the existence of cyclic dimeric guanosinemonophosphate (c-di-GMP) signaling pathway in streptococcus mutans. The pathway mediates the streptococcus mutans biofilm formation and in vitro adhesion to enamel surface.
OBJECTIVE: To analysis the effects of c-di-GMP on the biological properties of streptococcus mutans by gene chip technology.
METHODS: Exogenous c-di-GMP was used to interfere streptococcus mutans UA159. The total RNA was extracted, the total RNA of standard strain was as control, and then hybridized with streptococcus mutans whole-genome chips. The different genes were filtered.
RESULTS AND CONCLUSION: Expressions of 9 genes (glgA, glgB, msmF, gftA, lacG, lepB, rgpAc, bacC, apt6) were up-regulated while six genes (Hrc, ProC, HprT, Pdp, Glk, cdsA) expressions were down-regulated after exogenous c-di-GMP interfering. Differences genes were mainly related to the biological characteristics, such as cell tropism, biofilm formation, signal transduction, and energy metabolism. It showed that the c-di-GMP could affect the cariogenic ability of streptococcus mutans.

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