中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1421-1424.doi: 10.3969/j.issn.1673-8225.2012.08.020

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壳聚糖/磷酸三钙复合组织工程支架材料的制备及其性能*★

闫雪萍1，李  珊1，苏文昕2，宋  静1，何健民3   

  1. 1兰州大学口腔医学院，甘肃省兰州市  730000；2甘肃省第二人民医院，甘肃省兰州市  730000；3甘肃省人民医院口腔中心，甘肃省兰州市  730000
  • 收稿日期:2011-07-01 修回日期:2011-09-12 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 何健民，主任医师，甘肃省人民医院口腔中心，甘肃省兰州市 730000
  • 作者简介:闫雪萍★，女，1984年生，黑龙江省漠河县人，汉族，兰州大学在读硕士，主要从事口腔内科学方面的研究。 qichongtian2001@yahoo.com.cn
  • 基金资助:

    中央高校小额探索项目(223000-860164)。

Preparation and properties of chitosan/tricalcium phosphate composite scaffolds 

Yan Xue-ping1, Li Shan1, Su Wen-xin2, Song Jing1, He Jian-min3   

  1. 1Stomatological College of Lanzhou University, Lanzhou  730000, Gansu Province, China; 2Second People’s Hospital of Gansu, Lanzhou  730000, Gansu Province, China; 3Dental Center, Gansu Provincial Hospital, Lanzhou  730000, Gansu Province, China
  • Received:2011-07-01 Revised:2011-09-12 Online:2012-02-19 Published:2012-02-19
  • Contact: He Jian-min, Chief physician, Dental Center, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China
  • About author:Yan Xue-ping★, Studying for master’s degree, Stomatological College of Lanzhou University, Lanzhou 730000, Gansu Province, China qichongtian2001@ yahoo.com.cn
  • Supported by:

    Small Exploration Project of the Central Universities, No. 223000-860164*

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摘要：

背景：通过适当的工艺混合、加工来制备复合支架材料，可以弥补单一材料的不足，最大限度地满足组织工程的需要。
目的：制备壳聚糖/磷酸三钙复合支架，探讨其作为牙髓组织工程支架材料的可行性。
方法：壳聚糖粉末溶于微量冰醋酸溶液中，搅拌均匀，静置脱泡，预冷冻，交联，再次冷冻制成海绵状多孔壳聚糖/磷酸三钙支架。
结果与结论：冻干法制备的壳聚糖/磷酸三钙多孔支架平均孔隙率达85.78%，最高孔隙率达90%以上，孔径在100~300 μm，复合后的支架材料具有良好的韧性，当轴向压缩变形量超过5 mm时，材料仍然没有发生破坏。材料浸提液与牙髓细胞复合培养后，细胞毒性均为0级，由此可见壳聚糖/磷酸三钙复合材料具有良好的生物相容性、细胞亲和性和一定的力学性能，满足生物材料基本要求。

关键词: 壳聚糖, 磷酸三钙, 复合支架, 牙髓细胞, 生物相容性, 力学性能

Abstract:

BACKGROUND: Composite scaffolds prepared with appropriate mixing process can make up the insufficient of single material and satisfy the needs of tissue engineering in maximum.
OBJECTIVE: To prepare the chitosan/tricalcium phosphate composite scaffold and explore its feasibility of serving as scaffold for pulp tissue engineering.
METHODS: Chitosan powder was dissolved in small amount of acetic acid solution. The mixture was stirred to uniform, stilled to deaeration. Then pre-cooling and cross-linking were performed. Sponge-like chitosan/tricalcium phosphate composite scaffold was prepared after anther pre-cooling and cross-linking.
RESULTS AND CONCLUSION: Average porosity of the chitosan/tricalcium phosphate composite scaffold prepared by freeze-drying method was 85.78% and the highest porosity was over 90%. The aperture was 100-300 μm. The composite scaffold had a good toughness, and could not be damaged at over 5 mm of axial compression deformation. The cell toxicity was below grade 0 after cultured in the extracts of the materials. The chitosan/tricalcium phosphate composite scaffold can satisfy the basic needs of biomaterials based on its good biocompatibility, cell affinity, and some mechanical properties.

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