中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1345-1348.doi: 10.3969/j.issn.1673-8225.2012.08.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

聚乳酸/羟基磷灰石膜与人羊膜基质细胞联合构建骨组织工程细胞/支架复合体*☆◆

钟金晟1，梅  芳2，齐伟宏3，杨小平4   

  1. 1北京大学口腔医学院•口腔医院牙周科，北京市 100081；2北京大学医学部组织学与胚胎学教研室，北京市 100191；3卫生部北京医院妇产科，北京市 100730；4北京化工大学北京市新型高分子材料加工重点实验室，北京市  100029
  • 收稿日期:2011-12-23 修回日期:2012-01-11 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 梅芳，讲师，北京大学医学部组织学与胚胎学教研室，北京市 100191 meifang@ bjmu.edu.cn
  • 作者简介:梅芳，讲师，北京大学医学部组织学与胚胎学教研室，北京市 100191 meifang@ bjmu.edu.cn
  • 基金资助:

    国家自然科学基金项目(30471907)，课题名称：CNT/PLA/HA电纺丝纳米纤维支架在骨组织工程中的基础研究。

R318Combination application of human amniotic mesenchymal cells and poly (L-lactic acid)/ hydroxyapatite membranes for construction of cells/scaffold complexes in bone tissue engineering 

Zhong Jin-sheng1, Mei Fang2, Qi Wei-hong3, Yang Xiao-ping4   

  1. 1Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing  100081, China; 2Department of Histology and Embryology, Peking University Health Science Center, Beijing  100191, China; 3Department of Obstetrics and Gynecology, Beijing Hospital, Beijing  100730, China; 4The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemistry and Technology, Beijing  100029, China
  • Received:2011-12-23 Revised:2012-01-11 Online:2012-02-19 Published:2012-02-19
  • Contact: Mei Fang, Lecturer, Department of Histology and Embryology, Peking University Health Science Center, Beijing 100191, China meifang@ bjmu.edu.cn
  • About author:Zhong Jin-sheng☆, Doctor, Attending physician, Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China zhongjs@263.net
  • Supported by:

    the National Natural Science Foundation of China, No. 30471907*

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摘要：

背景：前期研究通过静电纺丝技术获得的聚乳酸/羟基磷灰石膜有利于细胞的贴附和生长。
目的：分析电纺聚乳酸/羟基磷灰石膜和人羊膜基质细胞构建骨组织工程细胞/支架复合体的可行性。
方法：利用MTT法检测聚乳酸和聚乳酸/羟基磷灰石膜浸提液对人羊膜基质细胞增殖的影响；将第3代人羊膜基质细胞培养于含聚乳酸和聚乳酸/羟基磷灰石膜的成骨诱导培养液中，进行组织学检查及免疫荧光细胞化学染色检测。
结果与结论：聚乳酸和聚乳酸/羟基磷灰石膜浸提液对人羊膜基质细胞均无明显细胞毒性。与两种膜材料复合培养后，人羊膜基质细胞细胞增殖明显，可观察到钙化结节的形成，钙化结节处细胞Ⅰ型胶原和碱性磷酸酶表达阳性，且聚乳酸/羟基磷灰石膜组细胞钙化结节数量及成熟程度优于聚乳酸组。说明电纺聚乳酸/羟基磷灰石膜与人羊膜基质细胞可以共同构建成细胞/支架复合体，具有应用于骨组织工程的潜力。

关键词: 聚乳酸, 羟基磷灰石, 人羊膜基质细胞, 成骨诱导, 骨组织工程

Abstract:

BACKGROUND: Previous study has shown that electrospun poly (L-lactic acid)/hydroxyapatite (PLLA/HA) membranes are suitable for cellular adhesion and proliferation.
OBJECTIVE: To investigate the feasibility of the human amniotic mesenchymal cells (hAMCs) and PLLA/HA membranes constructing the complex of seed cells/scaffold in bone tissue engineering.
METHODS: Effect of leaching liquor from the PLLA and PLLA/HA membranes on the proliferation of hAMCs was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay method. The third passage of hAMCs were cultured in the osteogenic induce media containing PLLA and PLLA/HA membranes, and then were detected by histological and immunofluorescence cytochemical staining.
RESULTS AND CONCLUSION: The leaching liquor of the PLLA and PLLA/HA had no cytotoxicity on the hAMCs. After seeded onto two kinds of the membranes, hAMCs proliferated actively and calcified nodules were observed. Cells in calcified nodules were shown positive expression of collagen type Ⅰ and alkaline phosphatase, and the number and maturity of the calcified nodules in the PLLA/HA group were better than those of the PLLA group. It is showed that the hAMCs and PLLA/HA can construct cells/scaffold complex and show a great potential of application in bone tissue engineering.

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