中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1229-1232.doi: 10.3969/j.issn.1673-8225.2012.07.021

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

老年大鼠模型视网膜血管改变及视网膜中央动脉血流变化的高分辨率超声图像检测★

许  晴1，路  欣2，史小林2   

  1. 首都医科大学，  1医学实验与测试中心，2组织胚胎学教研室，北京市   100069
  • 收稿日期:2011-11-05 修回日期:2011-12-28 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 史小林，教授，博士生导师，首都医科大学组织胚胎学教研室，北京市 100069 szhyxzhx@ccmu.edu.cn
  • 作者简介:许晴★，女，1969年生，北京市人，汉族，2010年首都医科大学毕业，硕士，高级实验师，主要从事小动物超声影像的研究。 xuqing@ccmu.edu.cn

Morphologic changes of retinal capillaries in aged rat models and blood velocity analysis in the central retinal artery using high-resolution ultrasound

Xu Qing1, Lu Xin2, Shi Xiao-lin2   

  1. 1Medical Experiments and Testing Center, 2Department of Histology and Embryology, Capital Medical University, Beijing  100069, China
  • Received:2011-11-05 Revised:2011-12-28 Online:2012-02-12 Published:2012-02-12
  • Contact: Shi Xiao-lin, Professor, Doctoral supervisor, Department of Histology and Embryology, Capital Medical University, Beijing 100069, China szhyxzhx@ccmu.edu.cnof
  • About author:Xu Qing★, Master, Medical Experiments and Testing Center, Capital Medical University, Beijing 100069, China xuqing@ccmu.edu.cn

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摘要：

背景：视网膜对缺血非常敏感，所以眼部血流动力学的改变可直接影响眼的功能，目前评估眼部血液循环可借助多种仪器设备。
目的：应用高分辨率小动物超声影像系统检测视网膜中央动脉的血流动力学变化，结合视网膜血管消化铺片技术检测视网膜血管结构变化，以明确老年大鼠视网膜中央动脉血流动力学的变化规律。
方法：使用高分辨率小动物超声影像系统测量老年大鼠和青年大鼠及视网膜中央动脉的血流参数，包括收缩期峰值血流速度、舒张末期血流速度，计算搏动指数、阻力指数和收缩期舒张期血流速度比值。同时使用视网膜血管消化铺片技术检测视网膜血管形态学改变。
结果与结论：与青年大鼠组相比，老年组大鼠视网膜血管内皮细胞增生，排列紊乱，管径增粗，血管壁不光滑；视网膜中央动脉血流速度、舒张末期血流速度均降低(P < 0.01)，计算搏动指数、阻力指数及收缩期峰值与舒张末期血流速度比值则升高(P < 0.01)。说明使用高分辨率小动物超声影像系统检测视网膜中央动脉收缩期和舒张期峰值速度及阻力指数能较敏感地反映血管的老化过程。

关键词: 视网膜中央动脉, 超声检查, 老年大鼠, 血流动力学, 毛细血管, 视网膜

Abstract:

BACKGROUND: The retina is more sensitive to ischemia. Therefore, the changes in ocular circulation can directly affect the function of the eye. Several tools have been found useful for the study of specific components of the ocular circulation
OBJECTIVE: To investigate the blood velocity in the central retinal artery (CRA) of the aged rat eye and morphologic changes in the aged retina using high-resolution small-animal ultrasound and combined with retinal vascular digest preparations.
METHODS: The blood flow parameters of CRA in aged and young rats were measured by high-resolution small-animal ultrasound imaging system; the parameters included the peak systolic velocity (PSV) and end diastolic velocity (EDV), the pulsatility index (PI), the resistive index (RI) and the systolic/diastolic (S/D) ratio were calculated. And the endothelial cell changes in the aged retina were determined by retinal vascular digest preparations.
RESULTS AND CONCLUSION: Compared with that of young adult rats, the retinal vasculature of aged rats showed morphologic proliferation and arrange disorder, broadening and the vessel wall was not smooth. PSV and EDV of the CRA was significantly lower (P < 0.01), PI, RI and S/D ratio were significantly higher (P < 0.01). Detection of PSV, EDV and resistance index of the CRA using high-resolution small-animal ultrasound provides more sensitive view of the blood vessels of the aging process.
 

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