中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 1050-1055.doi: 10.3969/j.issn.1673-8225.2012.06.023

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脊髓损伤亚急性期移植神经干细胞：损伤脊髓5-羟色胺的表达☆

巴迎春1，唐建中2，范  艳1，李仲铭1，王金德1，王廷华3   

  1. 1昆明医学院人体解剖学教研室，云南省昆明市   650500；2云南省第一人民医院肝胆外科，云南省昆明市  650032；  3昆明医学院神经科学研究所，云南省昆明市
    650500
  • 收稿日期:2011-08-20 修回日期:2011-11-16 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 王廷华，博士，教授，昆明医学院神经科学研究所，云南省昆明市 650500 Tinghua_neuron@ 263.net
  • 作者简介:巴迎春☆，男，1971年生，云南省麻栗坡县人，汉族，2010年昆明医学院毕业，博士，副教授，主要从事脊髓损伤研究。 byc71206@yahoo.com.cn

Neural stem cells transplantation in subacute stage of spinal cord injury: Expression of 5-hydroxytryptamine injured spinal cord  

Ba Ying-chun1, Tang Jian-zhong2, Fan Yan1, Li Zhong-ming1, Wang Jin-de1, Wang Ting-hua3   

  1. 1Department of Human anatomy, Kunming Medical College, Kunming  650500, Yunnan Province, China; 2Department of Hepatology, First People's Hospital of Yunnan Province, Kunming  650032, Yunnan Province, China; 3Institute of Neuroscience, Kunming Medical College, Kunming  650500, Yunnan Province, China
  • Received:2011-08-20 Revised:2011-11-16 Online:2012-02-05 Published:2012-02-05
  • Contact: Wang Ting–hua, Doctor, Professor, Institute of Neuroscience, Kunming Medical College, Kunming 650500, Yunnan Province, China Tinghua_neuron@263.net
  • About author:Ba Ying-chun☆, Doctor, Associate professor, Department of Human anatomy, Kunming Medical College, Kunming 650500, Yunnan Province, China byc71206@yahoo.com.cn

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摘要：

背景：5-羟色胺能纤维可以作为评价脊髓损伤后再生的指标之一。
目的：观察神经干细胞在脊髓损伤的亚急性期(第7天)脊髓内移植对大鼠受损伤脊髓再生修复的影响。
方法：将正常成年SD雌性大鼠分为3组，单纯脊髓全横断组、假手术组(仅仅打开椎板，但不横断脊髓)、神经干细胞移植组。神经干细胞移植组在脊髓全横断后第7天时进行神经干细胞移植，其他两组不移植神经干细胞。
结果与结论：神经干细胞移植组瘢痕上1至2节段5-羟色胺的阳性纤维数量多于单纯脊髓全横断组。提示神经干细胞亚急性期移植能部分促进脊髓损伤后脊髓神经的再生。
关键词：脊髓损伤；再生；神经干细胞；5-羟色胺；大鼠
doi:10.3969/j.issn.1673-8225.2012.06.023

关键词: 脊髓损伤, 再生, 神经干细胞, 5-羟色胺, 大鼠

Abstract:

BACKGROUND: 5-hydroxytryptamine (5-HT) serotonergic fibers are evaluated as one of regenerative indice after spinal cord injury.
OBJECTIVE: To study effects of neural stem cells (NSCs) transplantation on the recovery of the rats injured spinal cord in subacute stage (on the 7th day).
METHODS: The normal adult SD female rats were divided into 3 groups: merely spinal cord transection group, sham operation group (just opened the lamina, but not transected the spinal cord), NSCs transplantation group. NSCs transplantation group was performed on the 7th day after spinal cord transection, the other two groups were not performed with transplantation.
RESULTS AND CONCLUSION: In 1-2 segments above scar of spinal cord, the number of 5-HT positive fibers in NSCs transplantation group was more than that in merely spinal cord transection group. It indicates that NSCs transplantation group in subacute stage can promote the nerve regeneration after spinal cord injury.

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