中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 1032-1035.doi: 10.3969/j.issn.1673-8225.2012.06.019

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

静脉移植脐血干细胞可抑制脑缺血大鼠的神经细胞凋亡★

宋  红1，付  霞2，董蕾蕾1   

  1. 1沈阳医学院附属沈洲医院神经内科，辽宁省沈阳市 110002；2辽宁大学医院，辽宁省沈阳市  110036
  • 收稿日期:2011-07-21 修回日期:2011-09-30 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 付霞，硕士，主治医师，辽宁大学医院，辽宁省沈阳市 110036 fu-xia@sohu.com
  • 作者简介:宋红★，女，1975年生，辽宁省沈阳市人，汉族，2007年中国医科大学毕业，硕士，主治医师，主要从事缺血性脑血管病方面的研究。songhong_zyn@126.com

Intravenous administration of human umbilical cord blood stem cells reduces nerve cell apoptosis of cerebral ischemia rats

Song Hong1, Fu Xia2, Dong Lei-lei1   

  1. 1Department of Neurology, Shenzhou Hospital of Shenyang Medical College, Shenyang  110002, Liaoning Province, China; 2Liaoning University Hospital, Shenyang  110036, Liaoning Province, China
  • Received:2011-07-21 Revised:2011-09-30 Online:2012-02-05 Published:2012-02-05
  • Contact: Fu Xia, Master, Chief physician, Liaoning University Hospital, Shenyang 110036, Liaoning Province, China fu-xia@sohu.com
  • About author:Song Hong★, Master, Attending physician, Department of Neurology, Shenzhou Hospital of Shenyang Medical College, Shenyang 110002, Liaoning Province, China songhong_zyn@126.com

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摘要：

背景：已有实验证实脐血干细胞移植后可迁移至脑损伤区域，存活并分化为神经细胞。
目的：探讨经静脉移植脐血干细胞治疗大鼠脑缺血的疗效以及对神经细胞凋亡的影响。
方法：改良线栓法建立大鼠大脑中动脉闭塞再灌注模型，48只大鼠随机数字表法分成治疗组和对照组，分别在造模1 d后经尾静脉注射脐血干细胞和PBS进行观察。
结果与结论：治疗后3，7 d两组神经功能评分比较，差异无显著性意义(P > 0.05)；14，21 d治疗组神经功能评分明显低于对照组(P < 0.05)。对照组未见BrdU阳性细胞，治疗组大鼠脑组织切片中可见BrdU阳性细胞，对侧半球也可见少量BrdU阳性细胞。对照组大鼠缺血侧凋亡细胞显著多于治疗组(P < 0.05)。提示经静脉移植的脐血干细胞可迁移至大鼠缺血侧脑组织，抑制神经细胞凋亡，并显著改善大鼠神经功能。

关键词: 脑缺血, 脐血干细胞, 细胞移植, 神经细胞, 凋亡

Abstract:

BACKGROUND: After transplantation, the umbilical cord blood stem cells can migrate to the brain damage zone, survive and differentiate into nerve cells, promote recovery of damaged neurological function.
OBJECTIVE: To approach the curative effect of intravenous administration of human umbilical cord blood stem cells (hUCBSCs) on cerebral ischemia in rats, and its influence on nerve cells apoptosis.
METHODS: The middle cerebral artery occlusion (MCAO) model was established by using Zea-Longa’s method in rats. 48 rats were randomly divided into two groups: control group (n=24) and treatment group (n=24). Control group and treatment group were received intravenous administration of PBS and hUCBSCs, respectively, at 1 day after operation.
RESULTS AND CONCLUSION: There were no significant difference between the neurological scores of two groups at 3 and 7 days after operation (P >0.05); at the 14th and 21st days after operation, the neurological score was significantly improved in the treatment group compared with the control group (P < 0.05). Many BrdU-positive cells were found in the cerebral section in treatment group, while no BrdU-positive cells were found in control group. The number of apoptotic cells in treatment group was significantly less than that in the control group (P < 0.05). Intravenously administered hUCBSCs could migrate to the ischemia brain tissue, inhibit nerve cell apoptosis and improve the neurological function significantly.

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