关键词: 异种骨, 支架材料, 骨髓间充质干细胞, 细胞相容性, 细胞毒性

Abstract:

BACKGROUND: Antigen-extracted xenogeneic cancellous bone has been shown to be characterized by well physicochemical performance and three-dimensional stereo porous structure, however, the biocompatibility and safely still need to be further studied in clinical application. 
OBJECTIVE: To study the cytocompatibility of bone marrow mesenchymal stem cells (BMSCs) and antigen-extracted xenogeneic cancellous bone scaffold treated by chemical or physicochemical method.
METHODS: The gradient density centrifugation was used to separate and cultivate sheep BMSCs, the influence of antigen-extracted xenogeneic cancellous bone scaffold materials treated by chemical or physicochemical method on the proliferation of the BMSCs were measured by four thiazole salt colorimetric experiment (MTT method). The third generation of  BMSCs were taken and seeded on two scaffolds, the morphology, adhesion, growth and proliferation of the cells on the two scaffold materials were observed by inverted phase contrast microscope and scanning electron microscope.
RESULTS AND CONCLUSION: The cytotoxicity was 0 or 1 level in the physics joint chemical group, the cells exhibit well adhesion, proliferation and growth, cell activity was not affected by the material. The cytotoxicity was 3 level in chemical group, the cells were restrained, no cell adhesion in scaffold pores. The antigen-extracted xenogeneic cancellous bone materials treated with physical combined with chemical methods have good cytocompatibility to BMSCs. However, the scaffold materials treated by chemical methods have poor biocompatibility and it is not qualified for the safety standards of biological materials.