中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 766-770.doi: 10.3969/j.issn.1673-8225.2012.05.002

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

低强度脉冲超声波联合牙周组织翻瓣移植修复Beagle犬骨上缺损型
牙周病***★◆

李  娜1，卢  礼2，宋锦璘1，邓  锋1，赵纯亮3，王智彪3   

  1. 1重庆医科大学附属口腔医院冉家坝院区正畸科，重庆市  401147；  2解放军第三军医大学第二附属医院口腔科，重庆市   400037；3超声医学工程重庆市市级重点实验室，重庆市  400016
  • 收稿日期:2011-09-20 修回日期:2011-11-15 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 宋锦璘，博士，主任医师，教授，硕士生导师，重庆医科大学附属口腔医院冉家坝院区正畸科，重庆市 401147 soongjl@163.com
  • 作者简介:李娜★，女，1981年生，四川省眉山市人，汉族，重庆医科大学在读硕士，主治医师，主要从事口腔临床医学相关研究。
  • 基金资助:

    国家自然科学基金(30870754)；重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技 [2007]1110号)；重庆卫生局科研项目(2008-2-254)资助。

Low-intensity pulsed ultrasound combined with periodontal flap surgery for the repair of bone-defect periodontitis in Beagle dogs

Li Na1, Lu Li2, Song Jin-lin1, Deng Feng1, Zhao Chun-liang3, Wang Zhi-biao3   

  1. 1Department of Orthodontics, Brach of Ran Jia Ba, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing  401147, China; 2Department of Stomatology, the Second Affiliated Hospital, Third Military Medical University, Chongqing  400037, China; 3Chongqing Key Laboratory of Ultrasound Medical Engineering, Chongqing  400016, China
  • Received:2011-09-20 Revised:2011-11-15 Online:2012-01-29 Published:2012-01-29
  • Contact: Song Jin-lin, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthodontics, Brach of Ran Jia Ba, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China soongjl@163.com
  • About author:Li Na★, Studying for master’s degree, Attending physician, Department of Orthodontics, Brach of Ran Jia Ba, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30870754*; the Major High-Tech Industrial Technology Development Project of Chongqing Municipal Development and Reform Commission, No. 20071110*; Chongqing Health Bureau Research Project, No. 2008-2-254*

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摘要：

背景：近年来，低强度脉冲超声波联合牙周翻瓣对骨上缺损型牙周炎组织的修复效应的尚未见报道。
目的：观察低强度脉冲超声波联合牙周翻瓣术对Beagle犬骨上缺损型牙周炎组织的修复效应。
方法：在4只Beagle犬左下颌第二、三、四前磨牙处构建骨上缺损型牙周炎模型，经改良Widman翻瓣及根面处理后随机分为对照组(0 mW/cm2)和ISATA 30 mW/cm2×20 min/d、ISATA 60 mW/cm2×20 min/d低强度脉冲超声波处理组。
结果与结论：低强度脉冲超声波处理前后各组牙龈组织温度变化值差异无显著性意义(P > 0.05)；6周后对照组和低强度脉冲超声波处理组，以及处理组之间在牙周临床指标上差异无显著性意义(P > 0.05)；组织学切片苏木精-伊红染色显示低强度脉冲超声波处理组成骨细胞增生及骨陷窝较对照组明显，尤其是ISATA 30 mW/cm2×20 min/d低强度脉冲超声波处理组； Masson染色显示低强度脉冲超声波处理组红染较对照组明显；低强度脉冲超声波处理组和对照组均形成长结合上皮。提示低强度脉冲超声波具有潜在的促骨上缺损型牙周炎组织修复作用。

关键词: 低强度脉冲超声波, 牙周翻瓣术, Beagle犬, 骨缺损, 牙周炎组织

Abstract:

BACKGROUND: In recent years, there are no reports regarding tissue repair effects of low-intensity pulsed ultrasound (LIPUS) combined with periodontal flap surgery treatment on bone-defect periodontitis.
OBJECTIVE: To observe the effects of LIPUS combined with periodontal flap surgery on the repair of bone-defect periodontitis in Beagle dogs.
METHODS: Bone-defect periodontitis models were established in the left mandibular second, third and fourth premolars of four Beagle dogs. The models were randomly divided into a control group (0 mW/cm2) and two LIPUS treatment groups (ISATA
30 mW/cm2×20 min/d, ISATA 60 mW/cm2×20 min/d) after modified Widman flap surgery and root treatment.
RESULTS AND CONCLUSION: There was no significant difference in the change of gingival tissue’s surface temperature before and after LIPUS irradiation (P > 0.05). There was no significant difference of periodontal clinical parameters in the LIPUS and control groups, as well as between the two LIPUS groups after 6 weeks treatment (P > 0.05). Histological analysis (hematoxylin- eosin staining) indicated that osteoblast proliferation and bone lacunae in the LIPUS groups, especially in ISATA 30 mW/cm2×    20 min/d group, were more mature than those in the control group. Masson staining showed red staining was more obvious in the LIPUS groups than in the control group. Long-junction epithelium was found in all the three groups. LIPUS may have some potential reparative effects on the promotion of alveolar bone maturation.

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