中国组织工程研究

中国组织工程研究 ›› 0, Vol. ›› Issue (0): 125-129.doi: 10.3969/j.issn.1673-8225.2012.01.027

• 骨髓干细胞 • 上一篇    下一篇

小型猪富血小板血浆对牙周膜干细胞的成骨诱导

张  远1，钟良军2，张鹏涛1，张源明3，徐  艳4
  

  1. 1 新疆医科大学第一附属医院口腔系，新疆维吾尔自治区乌鲁木齐市  830011； 2杭州师范大学临床医学院，浙江省杭州市  310015； 3新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市  830054； 4南京医科大学附属口腔医院，江苏省南京市  210029
  • 收稿日期:2011-09-23 修回日期:2011-10-14
  • 通讯作者: 张源明，教授，博士生导师，新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市 830054 zymdxx@163.com
  • 作者简介:张远★，男，1984年出生，陕西汉中人，新疆医科大学在读硕士，主要从事牙周病防治的研究。 ctionzy@qq.com

Effect of miniature pig platelet-rich plasma on osteogenic induction of periodontal ligament stemcells in vitro

Zhang Yuan1, Zhong Liang-jun2, Zhang Peng-tao1, Zhang Yuan-ming3, Xu Yan4
  

  1. 1Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China; 2Clinical Medical College of Hangzhou Normal University, Hangzhou  310015, Zhejiang Province, China; 3Heart Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 4 Affiliated Dental Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
     
  • Received:2011-09-23 Revised:2011-10-14
  • Contact: Zhang Yuan-ming, Professor, Doctoral supervisor, Heart Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zymdxx@163.com
  • About author:Zhang Yuan★, Studying for master’s degree, Department of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China ctionzy@qq.com

PDF

387

被引次数

8

摘要：

背景：组织工程技术为牙周炎致骨组织缺损的修复提供了新的思路。
目的：探寻富血小板血浆在小型猪牙周膜干细胞成骨诱导中的作用。
方法：采集贵州小型猪静脉血，三次离心制备富血小板血浆。采用组织块法分离培养小型猪牙周膜干细胞，分别将含体积分数0.8%，1.0%，1.2%的富血小板血浆与牙周膜干细胞共同培养3，7，14，21 d，以未添加富血小板血浆的牙周膜干细胞作为对照。
结果与结论：体积分数0.8%，1.0%，1.2%的富血小板血浆成骨诱导第14天，碱性磷酸酶活性达到峰值，其中体积分数1.0%的富血小板血浆诱导的细胞碱性磷酸酶活性最高，第21天时碱性磷酸酶活性降低。茜素红染色显示富血小板血浆成骨诱导第21天细胞染色呈阳性。单克隆纯化后细胞的生长曲线从第3天起进入对数生长期，第8天细胞数量达到顶峰。说明富血小板血浆具有诱导牙周膜干细胞成骨的能力，以体积分数1.0%时诱导成骨效率最优。

关键词: 牙周膜干细胞, 小型猪, 富血小板血浆, 成骨诱导, 碱性磷酸酶

Abstract:

BACKGROUND: Tissue engineering technology provides a new way for bone defects caused by periodontitis.
OBJECTIVE: To explore the effect of platelet-rich plasma (PRP) on osteogenic induction of miniature pig periodontal ligament stem cells (PDLSCs).
METHODS: Collected the Guizhou miniature pig’s venous blood and prepared the centrifuged PRP three times; tissue block method was used to culture the periodontal ligament cells. 0.8%, 1.0%, 1.2% concentration of PRP were co-cultured with PDLSCs for 3, 7, 14 and 21 days, respectively. No PRP addition as control group.
RESULTS AND CONCLUSION: Alkaline phosphatase (AKP) activity reach a peak at the 14th day after induced with 0.8%, 1.0% and 1.2% PRP, and highest activity of AKP was induced by 1.0% PRP. AKP activity was reduced at the 21st day. Alizarin red staining showed the PDLSCs were positive at 21st day after osteogenic induction of PRP. Purified monoclonal cell growth curve entering the logarithmic growth phase since the 3rd day, the amount of cells reached a peak at the 8th day. PRP can induce osteogenic PDLSC performance, especially the 1.0% PRP induction showed the optimal efficiency in vitro.

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