中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9453-9456.doi: 10.3969/j.issn.1673-8225.2011.50.035

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

大鼠正畸牙移动中Toll样受体4在牙周组织中的表达

聂  晶1，钟良军2，李小兵3，林宝山3，钱  煦3，钱雅婧1   

  1. 1新疆医科大学第一附属医院，口腔正畸科，新疆维吾尔自治区乌鲁木齐市  830054
    2杭州师范大学附属医院口腔科，浙江省杭州市 310015
    3四川大学华西口腔医院口腔正畸科，四川省成都市610041
  • 收稿日期:2011-09-07 修回日期:2011-11-09 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 钟良军，主任医师，杭州师范大学附属医院口腔科，浙江省杭州市310015 zymdxx@163.com
  • 作者简介:聂晶★，男，1980年生，河南省扶沟县人，汉族，2007年新疆医科大学毕业，硕士，主要从事错牙合畸形防治的研究。 niejing202@sohu.com
  • 基金资助:

    新疆医科大学第一附属医院青年专项基金(2010-QN-09)，课题名称：大鼠正畸牙移动中TLR-4在牙周组织中表达与分布的研究。

Expression of Toll-like receptor 4 in rat periodontal ligament during orthodontic tooth movement

Nie Jing1, Zhong Liang-jun2, Li Xiao-Bing3, Lin Bao-Shan3, Qian Xu3, Qian Ya-Jing1   

  1. 1Department of Orthodontics, First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
    2Department of Stomatology, the  Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China
    3Department of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-09-07 Revised:2011-11-09 Online:2011-12-10 Published:2011-12-10
  • Contact: Zhong Liang-jun, Chief physician, Department of Stomatology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China zymdxx@163.com
  • About author:Nie Jing★, Master, Department of Orthodontics, First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China niejing202@sohu.com
  • Supported by:

    the Youth Foundation of the First Affiliated Hospital of Xinjiang Medical University, No. 2010-QN-09*

PDF

396

被引次数

2

摘要：

背景：学者们对正畸力作用下牙周组织的改建做了大量的研究，但对于在大鼠牙齿移动过程中Toll样受体4在牙周组织中表达的研究尚未涉及。
目的：研究在正畸牙移动过程中，大鼠牙周组织中Toll样受体4的表达。
方法：采用NiTi拉簧建立大鼠正畸牙移动模型，力值50 g。分别于加力后1，3，5，7，10，14 d取正畸牙周组织，检测Toll样受体4的表达。
结果与结论：免疫荧光染色显示，正畸加力1 d，牙周组织中Toll样受体4的表达量增强，至加力后5 d时表达量最大，之后逐渐降低，第14天时回落至初始水平。可见正畸力可以刺激牙周组织中Toll样受体4的表达，Toll样受体4可能参与了牙周组织的改建。

关键词: 牙周组织, Toll样受体4, 正畸牙移动, 细胞因子, 组织改建

Abstract:

BACKGROUND: Extensive research has been done on the function of the periodontal ligament under the orthodontic force. However, there are no reports regarding the expression of Toll-like receptor 4 in rat periodontium during orthodontic tooth movement.
OBJECTIVE: To investigate the expression of Toll-like receptor 4 in rat periodontium during orthodontic tooth movement.
METHODS: Rat orthodontic tooth movement model was constructed using NiTi extension spring with the force value of 50 g. The orthodontic periodontium was collected on the 1st, 3rd, 5th, 7th, 10th and 14th days after exerting force to detect the expression of Toll-like receptor 4.
RESULTS AND CONCLUSION: According to immunofluorescence staining, the expression of Toll-like receptor 4 in the periodontal tissues increased on the 1st day after exerting force, and reached highest level on the 5th day; then fell to the initial level on the 14th day. Thus the expression of Toll-like receptor 4 is evoked by orthodontic force, and it may play a role in periodontal remodeling.

中图分类号: