中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8821-8824.doi: 10.3969/j.issn.1673-8225.2011.47.020

• 药物控释材料 drug delivery materials • 上一篇    下一篇

丝素蛋白/壳聚糖三维支架材料的制备方法

佘荣峰，邓  江，黄文良，苑  成，董志军   

  1. 遵义医学院第三附属医院骨科，贵州省遵义市 563003
  • 收稿日期:2011-04-14 修回日期:2011-08-16 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 邓江，主任医师，研究生导师，遵义医学院第三附属医院骨科，贵州省遵义市 563003
  • 作者简介:佘荣峰★，男，1984年生，贵州省遵义市人，汉族，遵义医学院在读硕士，主要从事骨与关节创伤方面的研究。
  • 基金资助:

    贵州省科学技术基金项目(黔科合J字[2009]2172号)，课题名称：SF-CS复合诱导骨髓间充质干细胞修复兔膝关节软骨缺损的研究；贵州省社会攻关项目(黔科合SY字[2010]3101号)，课题名称：SF-CS-nHA支架与基因修饰后BMSCs构建人工骨种植体的成骨作用研究。

Preparation of three-dimensional silk fibroin/chitosan scaffolds

She Rong-feng, Deng Jiang, Huang Wen-liang, Yuan Cheng, Dong Zhi-jun   

  1. Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2011-04-14 Revised:2011-08-16 Online:2011-11-19 Published:2011-11-19
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China DJ30666@126.com
  • About author:She Rong-feng★, Studying for master’s degree, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Guizhou Province, No. [2009]2172*; the Social Tackle Key Program of Guizhou Province, No. [2010]3101*

PDF

548

被引次数

24

摘要：

背景：很多研究表明丝素蛋白、壳聚糖为天然高分子材料，无毒无味，有良好的生物特性和理化性质。
目的：探讨符合软骨组织工程支架材料要求的丝素蛋白/壳聚糖三维支架材料制备方法。
方法：将丝素蛋白与壳聚糖按质量比分别为3∶1，1∶1，1∶3，0∶1的比例混合制备丝素蛋白-壳聚糖复合材料，通过孔径大小、孔隙率、吸水膨胀率及热水溶失率的测定，寻找丝素蛋白/壳聚糖最佳混合比例。
结果与结论：丝素蛋白/壳聚糖按质量1∶1的比例混合更符合要求：孔径90~280 μm，平均孔径为151.72 μm；孔隙率为(92.72±4.78)%；吸水膨胀率为(141.10±6.87)%；热水溶失率交联后较交联前降低，交联前后比较差异有显著性意义(P < 0.05)。说明丝素蛋白/壳聚糖按1∶1复合支架材料符合软骨组织工程支架材料理化性质的要求，该材料有望作为软骨组织工程研究较理想的支架材料。

关键词: 丝素蛋白, 壳聚糖, 支架, 组织工程, 材料

Abstract:

BACKGROUND: Many studies have shown that silk fibroin (SF) and chitosan (CS) as natural polymer materials are non-toxic and tasteless, and they have good physical and chemical properties and biological characteristics.
OBJECTIVE: To discuss the preparation methods of three-dimensional SF/CS scaffolds which meet the requirements of cartilage tissue engineering scaffolds.
METHODS: SF and CS were mixed at different ratios such as 3 to 1, 1 to 1, 1 to 3 and 0 to 3, then to make composite materials. In order to find out the best mixing ratio of SF and CS, the following indexes including pore size, porosity, the water absorption and loss rate in hot water were determined.
RESULTS AND CONCLUSION: It was more suitable to meet the requirements when the mixed ratio of SF to CS was 1 to 1. The pore size was 90-280 μm and the average pore size was 151.72 μm. The porosity was (92.72±4.78)%. The water absorption was (92.72±4.78)%. The loss rate in hot water after crosslinking was decreased compared before crosslinking, and there was a significant difference (P < 0.05). The mixed ratio of SF to CS is at 1 to 1 meeting the physicochemical requirements of cartilage tissue engineering scaffolds. The SF/CS composite material is expected to be an ideal scaffold material for cartilage tissue engineering.

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