中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8653-8656.doi: 10.3969/j.issn.1673-8225.2011.46.024

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

不同保存方式下嗅鞘细胞的活性

刘雨亮1，魏开斌1，刘  红2，何玉芹2，卓  峰1，吕昕刚3   

  1. 泰安市中心医院，1骨一科，2护理部，山东省泰安市  271000；3新汶矿务局莱芜中心医院骨一科，山东省莱芜市  271100
  • 收稿日期:2011-01-19 修回日期:2011-05-18 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 魏开斌，博士，主任医师，泰安市中心医院骨一科，山东省泰安市 271000
  • 作者简介:刘雨亮★，男，1974年生，山东省单县人，汉族，2010年泰山医学院毕业，硕士，主治医师，主要从事脊髓损伤与修复的研究。
  • 基金资助:

    山东省医药卫生科技发展计划项目(课题编号:2009HW091)。

Olfactory ensheathing cell viability in different cryopreservation systems

Liu Yu-liang1, Wei Kai-bin1, Liu Hong2, He Yu-qin2, Zhuo Feng1 , Lü Xin-gang3   

  1. 1First Department of Orthopedics, 2Nursing Department, Taian Central Hospital, Taian  271000, Shandong Province, China; 3First Department of Orthopedics, Xinwen Coal Mining Bureau Laiwu Central Hospital, Laiwu  271100, Shandong Province, China
  • Received:2011-01-19 Revised:2011-05-18 Online:2011-11-12 Published:2011-11-12
  • Contact: Wei Kai-bin, Doctor, Chief physician, First Department of Orthopedics, Taian Central Hospital, Taian 271000, Shandong Province, China wkb1966@163.com
  • About author:Liu Yu-liang★, Master, Attending physician, First Department of Orthopedics, Taian Central Hospital, Taian 271000, Shandong Province, China yuliang-liu@163.com
  • Supported by:

    the Science and Technology Development Plan Project of the Medicine and Health of Shandong Province, No. 2009HW091*

PDF

433

被引次数

4

摘要：

背景：获取和寻找适合的保存方式对嗅鞘细胞实验和临床应用有重要的意义。
目的：探索合适的嗅鞘细胞低温保存方式。
方法：取对数期生长的嗅鞘细胞，冻存1，3，6个月进行复苏。
结果与结论：MTT比色法及锥虫蓝染色显示5%二甲基亚砜-6%羟乙基淀粉处理的细胞活性最高，其次是10%二甲基亚砜处理的细胞，5%二甲基亚砜的保护作用最差。冰箱降温或程控降温仪降温方式及不同的冻存时间对嗅鞘细胞活性影响不明显。因此，推荐用5%二甲基亚砜-6%羟乙基淀粉作为嗅鞘细胞冻存低温保护剂。

关键词: 嗅鞘细胞, 低温保护剂, 二甲基亚砜, 羟乙基淀粉, 细胞活性, 保存方式, 细胞复苏

Abstract:

BACKGROUND: A proper preservation method would be of important significance for experiments and clinical application of olfactory ensheathing cells (OECs)
OBJECTIVE: To explore proper cyropreservative systems for OECs.
METHODS: OECs during the logarithmic growth phase were harvested, cryopreserved for 1, 3 and 6 months and then revitalized.
RESULTS AND CONCLUSION: MTT assay and tryplan blue staining showed that cells exhibited highest viability after treatment with 5% dimethyl sulfoxide (DMSO)-6% hydroxyethyl starch (HES), followed by 10% DMSO, and lastly the 5% DMSO. Use of refrigerator or cryogenic control system with different cryopreservation time did not yield obvious effects on viability of OECs. Therefore, 5% DMSO-6%HES is recommended as a cryopreservative agent for OECs.

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