中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8366-8370.doi: 10.3969/j.issn.1673-8225.2011.45.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

静水压刺激人骨髓间充质干细胞骨架的排列

何  川1，2，梁  静2，邓廉夫2，杨庆铭1，2   

  1. 1上海交通大学医学院附属瑞金医院骨科，上海市 200025
    2上海市伤骨科研究所，上海市  200025
  • 收稿日期:2011-04-25 修回日期:2011-08-11 出版日期:2011-11-05 发布日期:2011-11-05
  • 作者简介:何川☆，男，1971年生，四川省泸州市人，汉族，2005年上海第二医科大学毕业，博士，副主任医师，主要从事关节外科方面的研究。 drhechuan@sina.com
  • 基金资助:

    国家自然科学基金项目资助(81071473)，课题名称：间充质干细胞向软骨细胞诱导分化过程中低氧/ HIF-1α的调控作用。

Effect of hydrostatic compressive loading on the cytoskeletal structure of human bone marrow mesenchymal stem cells

He Chuan1,2, Liang Jing2, Deng Lian-fu2, Yang Qing-ming1,2   

  1. 1Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai  200025, China
    2Shanghai Institute of Traumatology and Orthopedics, Shanghai  200025, China
  • Received:2011-04-25 Revised:2011-08-11 Online:2011-11-05 Published:2011-11-05
  • About author:He Chuan☆, Doctor, Associate chief physician, Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai 200025, China; Shanghai Institute of Traumatology and Orthopedics, Shanghai 200025, China drhechuan@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071473*

PDF

376

被引次数

3

摘要：

背景：力学信号如何调节骨髓间充质干细胞分化的机制尚不清楚，细胞骨架结构变化可能在力学信号细胞内传导中起重要作用。
目的：分析静水压作用下人骨髓间充质干细胞骨架蛋白丝状肌动蛋白微丝的空间结构变化以及阻断ERK1/2信号通路或整合素α2β1后对这一过程的影响。
方法：利用加压容器对体外培养的人骨髓间充质干细胞施加40 kPa或80 kPa压强的静水压力刺激，加载时间为1 h或4 h，细胞培养也中分别加入10 mmol/LERK1/2信号通路抑制剂U0126或10 mg/L 整合素α2β1抗体以阻断细胞内ERK1/2信号通路或细胞表面整合素α2β1受体。
结果与结论：受压后骨髓间充质干细胞骨架微丝比不进行干预的细胞纤细，并且散乱分布于胞浆内、无方向性；与受压1 h相比，受压4 h的细胞骨架微丝形态、分布和排列更接近未受压组；U0126比整合素α2β1抗体更明显地阻断骨髓间充质干细胞在静水压刺激下的细胞骨架微丝重组现象。说明人骨髓间充质干细胞在静水压刺激下细胞骨架结构发生重组和重排，ERK1/2信号途径起重要作用。

关键词: 细胞骨架, 静水压, 骨髓, 间充质干细胞, 肌动蛋白微丝

Abstract:

BACKGROUND: Although little is known about the molecular mechanisms by which mechanical signals regulate mesenchymal stem cells (MSCs) differentiation, cytoskeleton may play an important role in procedure of intracellular mechanical signal delivering.
OBJECTIVE: To investigate the change of the spatial structure of filamentous actin (F-actin), a cytoskeleton protein, under the continuous hydrostatic pressure and the effects of ERK1/2 signaling pathway or integrin α2β1 block on the above-mentioned process.
METHODS: In vitro cultured hBMSCs were incubated under continuous pressure of 40 or 80 kPa for 1 or 4 hours by hydraulic pressure controlled cellular strain unit, with or without blocking the ERK1/2 signaling pathway by 10 mmol/L U0126 or by 10 mg/L anti-integrin α2β1 antibody.
RESULTS AND CONCLUSION: The actin filaments of the strained BMSCs were more flimsy and tenuous than untreated osteoblasts and unlike the normal distribution of bundles or membrane-like of the control group, and they were arranged without direction. But after stained for 4 hours cells showed an arrangement more near the unstained cells than after stained for 1 hour. The U0126 interrupted the rearrangement phenomenon of BMSCs under hydraulic pressure more severely than the anti-integrin α2β1 antibody did. BMSCs under hydrostatic compressive loading lead to cytoskeletal reorganization and rearrangement; The ERK1/2 signaling pathway plays an important role in the process.

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