中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8371-8375.doi: 10.3969/j.issn.1673-8225.2011.45.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞下调类风湿性关节炎小鼠脾脏单个核细胞TLR8及TLR9的表达

毛  飞，钱  晖，朱  伟，严永敏，高  硕，许文荣   

  1. 江苏大学基础医学与医学技术学院，江苏省镇江市   212013
  • 收稿日期:2011-04-08 修回日期:2011-06-21 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 许文荣，博士，教授，江苏大学基础医学与医学技术学院，江苏省镇江市 212013 icls@ujs.edu.cn
  • 作者简介:毛飞☆，1979年生，男，江苏省泗洪县人，2009年江苏大学毕业，博士，讲师，主要从事血液学与血液学检验方面的工作。 maofei2003@ujs.edu.cn
  • 基金资助:

    国家自然科学青年基金项目(81000181)，课题名称：小鼠骨髓MSC调控单核/巨噬细胞修复肝损伤机制的研究。江苏省高校自然科学研究项目(10KJB310002) ，课题名称：小鼠骨髓MSC调节单核/巨噬细胞修复肝损伤的机制研究。江苏大学高级专业人才科研启动基金项目(10JDG094)，课题名称：LacZ转基因小鼠骨髓MSC调节单核/巨噬细胞修复肝损伤的机制研究。江苏大学拔尖人才和科技创新团队项目(2008-018-02)。

Effect of bone marrow mesenchymal stem cells on down-regulation of Toll-like receptor 8 and Toll-like receptor 9 in spleen mononuclear cells in collagen-induced arthritis mice

Mao Fei, Qian Hui, Zhu Wei, Yan Yong-min, Gao Shuo, Xu Wen-rong   

  1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang  212013, Jiangsu Province, China
  • Received:2011-04-08 Revised:2011-06-21 Online:2011-11-05 Published:2011-11-05
  • Contact: Xu Wen-rong, Doctor, Professor, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China icls@ujs.edu.cn
  • About author:Mao Fei☆, Doctor, Lecturer, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China maofei2003@ujs.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 81000181*; the Natural Science Foundation of Jiangsu Universities, No. 10KJB310002*; the Science and Technology Start-up Foundation for Advance Talents of Jiangsu University, No. 10JDG094*; Top-Notch Talent and Technological Innovation Team Project of Jiangsu University, No. 2008-018-02*

PDF

476

被引次数

16

摘要：

背景：间充质干细胞能够缓解类风湿性关节炎小鼠的症状，但是其机制还不清楚。
目的：观察骨髓间充质干细胞对类风湿性关节炎小鼠脾脏单个核细胞表达TLR8及TLR9等的影响。
方法：DBA/1J小鼠随机分3组，非造模组不造模，阳性对照组和实验组制备Ⅱ型胶原诱导的小鼠类风湿性关节炎模型。实验组尾静脉注射移植大鼠骨髓间充质干细胞。
结果与结论：与阳性对照组比较，实验组小鼠关节直径明显减小，关节炎症细胞浸润程度明显降低，但比非造模组略高；小鼠脾脏单个核细胞表达TLR8、TLR9和白细胞介素1β的水平较阳性对照组明显降低(P < 0.01或P < 0.05)；阳性对照组与实验组中TLR8与TLR9的表达均无明显相关性(P > 0.05)。说明骨髓间充质干细胞下调了类风湿关节炎小鼠脾脏单个核细胞表达TLR8及TLR9的水平，但TLR8与TLR9的表达无相关性。

关键词: 类风湿性关节炎, 骨髓间充质干细胞, TLR8, TLR9, 小鼠

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) can attenuate the symptoms of collagen-induced arthritis (CIA) mice but the mechanism of which is not clear.
OBJECTIVE: To explore the effect of MSCs on the expression of Toll-like receptor 8 (TLR8), Toll-like receptor 9 (TLR9) and so on in spleen mononuclear cells in CIA mice.
METHODS: DBA/1J mice were randomly divided into sham-operation group, positive control group and test group. Chicken Ⅱ collagen was used to establish the model of CIA in mice. Rat MSCs were administered into the test group through intravenous injection.
RESULTS AND CONCLUSION: The joint diameter of the test mice decreased obviously compared with that of positive control group. Severe inflammation was detected in the positive group mice by histological analysis, which was not seen in the test group and negative group. The expression levels of TLR8, TLR9 and interleukin-1β in spleen mononuclear cells from the test group were decreased compared with those from the positive group which were higher than that from the negative group (P < 0.01, P < 0.05). Statistical analysis showed that there was no correlation between the expression of TLR8 and TLR9 in the positive control group and test group (P > 0.05). The expression of TLR8 and TLR9 in spleen mononuclear cells from CIA mice were decreased by MSCs, and there was no correlation between the expression of TLR8 and TLR9.

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