中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8305-8307.doi: 10.3969/j.issn.1673-8225.2011.44.034

• 移植与基因 transplantation and gene • 上一篇    下一篇

中国辽宁锡伯族群体3个短串联重复位点的遗传多态性分析

刘  健，张  威，尹娇杨，高  兵，郭  丽，祁  荣，田秀荣   

  1. 沈阳医学院细胞生物与遗传学教研室，辽宁省沈阳市  110034 
  • 收稿日期:2011-05-19 修回日期:2011-07-26 出版日期:2011-10-29 发布日期:2011-10-29
  • 作者简介:刘健☆，女，1963年生，辽宁省沈阳市人，汉族，2007年中国医科大学毕业，博士，副教授，主要从事人类群体分子遗传学研究。 liujian910709@163.com
  • 基金资助:

    沈阳医学院科学研究基金(编号：20081026)，课题名称：辽宁锡伯族群体6个STR基因座遗传多态性研究。

Genetic polymorphisms of three short tandem repeat loci in Liaoning Xibo population of China

Liu Jian, Zhang Wei, Yin Jiao-yang, Gao Bing, Guo Li, Qi Rong, Tian Xiu-rong   

  1. Department of Cell Biology and Genetics, Shenyang Medical College, Shenyang  110034, Liaoning Province, China
  • Received:2011-05-19 Revised:2011-07-26 Online:2011-10-29 Published:2011-10-29
  • About author:Liu Jian☆, Doctor, Associate professor, Department of Cell Biology and Genetics, Shenyang Medical College, Shenyang 110034, Liaoning Province, China liujian910709@163. com
  • Supported by:

    Science Research Foundation of Shenyang Medical College, No. 2081026*

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摘要：

背景：对常染色体STR基因座的多态性的研究可为法医学亲权鉴定提供基础数据。
目的：探索辽宁锡伯族D16S539，THO1，D13S317 3个常染色体基因座的遗传多态性，建立锡伯族群体的遗传学基础数据。
方法：采集辽宁省沈阳市新城子区黄家乡锡伯族中小学的150名中小学生口腔黏膜细胞，Chelex 100法提取DNA，进行荧光标记PCR扩增，产物在Li-COR 4300基因分析仪上进行电泳，E-seq分析软件计算扩增产物片段相对大小，进行基因型分型。调查辽宁地区锡伯族群体 3个 STR基因座等位基因频率，进行遗传多态性分析。
结果与结论：辽宁锡伯族群体中3个STR基因座具有遗传多态性，其基因型分布符合Hardy-Weinberg平衡定律。辽宁锡伯族群体中3个STR基因座的杂合度分布在0.769~0.810；个人识别力分布在0.824~0.929，累积个人识别能力为0.999；多态信息量分布在 0.650~0.790；非父排除率分布在0.565~0.790，累积非父排除率为0.979。说明辽宁锡伯族群体3个常染色体STR基因座有较高的非父排除率和个体识别能力，可为法医学亲子鉴定和个体识别及移植配型等遗传学研究提供依据。

关键词: 锡伯族, 短串联重复, D16S539, THO1, D13S317, 基因频率, 多态性

Abstract:

BACKGROUND: Studying the gene polymorphism of autosome short tandem repeat (STR) gene loci can provide basic data for forensic paternal power identification. 
OBJECTIVE: To explore the genetic polymorphism of three STR loci, D16S539, THO1, D13S317, in Liaoning Xibo population of China and establish the genetic basic data of Xibo population.
METHODS: Buccal samples were obtained from 150 primary and middle school students of Xibo population living in Liaoning province and PCR amplification reactions of each STR loci were performed in fluorescence-based reaction using a 2720 Thermal cycler. The amplified products were detected in a Li-COR 4300 DNA Analyzer. Relative fragment size of amplified products was calculated through the use of E-seq Analysis software and genotyping was performed. Allele frequency of three STR genetic loci of Xibo population living in Liaoning province was investigated and genetic polymorphism analysis was performed.
RESULTS AND CONCLUSION: All the three loci met the Hardy-Weinberg equilibrium. Heterozygosis degree of these three loci was distributed between 0.769-0.810. Individual discriminative power was distributed between 0.824-0.929. Cumulative individual discriminative power was 0.999. Polymorphism information was distributed between 0.650-0.790. Non-parental eliminant rate was distributed between 0.565-0.790. Cumulative non-parental eliminant rate was 0.979. The three STR loci used in this study show high non-parental eliminant rate and individual discriminative power in Xibo population and can be applied to population study and forensic practice.

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