中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (42): 7843-7846.doi: 10.3969/j.issn.1673-8225.2011.42.013

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米可降解聚乳酸-羟基乙酸尿道支架的制备及性能

王晓庆1，姜凤鸣1，侯宇川1，王春喜1，张  龙1，陈岐辉1，张海峰1，高站团2，汪  岩1   

  1. 1吉林大学白求恩第一医院泌尿外科，吉林省长春市  130021
    2中国科学院长春应用化学研究所，吉林省长春市  130022
  • 收稿日期:2011-07-13 修回日期:2011-08-22 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 汪岩，副教授，吉林大学白求恩第一医院泌尿外科，吉林省长春市130021 39044355@ qq.com
  • 作者简介:王晓庆☆，男，1981年生，吉林省长岭县人，汉族，吉林大学在读博士，主治医师，主要从事泌尿系统可降解材料的应用研究。 upw018@ 126.com
  • 基金资助:

    国家自然科学基金项目(50973043)，课题名称：梯度可降解输尿管支架的研制。

Fabrication and properties of nano-biodegradable poly(lactic-co-glycolic acid) tubular urethral stents 

Wang Xiao-qing1, Jiang Feng-ming1, Hou Yu-chuan1, Wang Chun-xi1, Zhang Long1, Chen Qi-hui1, Zhang Hai-feng1, Gao Zhan-tuan2, Wang Yan1   

  1. 1Department of Urology, First Bethune Hospital of Jilin University, Changchun  130021, Jilin Province, China;
    2State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun  130022, Jilin Province, China
  • Received:2011-07-13 Revised:2011-08-22 Online:2011-10-15 Published:2011-10-15
  • Contact: Wang Yan, Associate professor, Department of Urology, First Bethune Hospital of Jilin University, Changchun 130021, Jilin Province, China 39044355@qq.com
  • About author:Wang Xiao-qing☆, Studying for doctorate, Attending physician, Department of Urology, First Bethune Hospital of Jilin University, Changchun 130021, Jilin Province, China upw018@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50973043*

PDF

349

被引次数

7

摘要：

背景：聚乳酸-羟基乙酸可作为尿道替代物进行组织缺损的修复。
目的：观察电纺丝法制备聚乳酸-羟基乙酸共聚物可降解尿道支架的可行性，并评价支架管的体外降解性能。
方法：采用电纺丝技术制备纳米聚乳酸-羟基乙酸共聚物(摩尔比80∶20)尿道支架管，并以戊二醛对支架进行交联、改性，将交联后支架截成长约1 cm小段并浸于尿液中进行体外降解实验。
结果与结论：支架管具有纳米结构，孔隙率约89%，孔径(32±19) µm；交联后可见纤维表面变粗糙，但纤维丝直径、孔径及孔隙率与交联前差异无显著性意义(P > 0.05)，但交联后支架管力学性能显著提高。支架降解初期速度相对较快，中后期降解速度减慢，至8周时材料质量损失约50%，第10周完全崩解。材料在体内降解过程中相对分子质量的变化趋势与质量损失大体相同，降解早期相对分子质量下降相对较快，后期下降速度减慢并趋于平稳。表明采用电纺丝技术制备的纳米聚乳酸-羟基乙酸共聚物尿道支架可满足尿道组织工程支架的要求。

关键词: 电纺丝, PLGA(聚乳酸-羟基乙酸共聚物80∶20), 尿道支架, 可降解, 纳米结构

Abstract:

BACKGROUND: Poly (lactic-co-glycolic acid) (PLGA) can be used as a substitute for urethral tissues.
OBJECTIVE: To discuss the feasibility of the fabrication of co-polymer PLGA 80:20 tubular urethral stents by using electrospinning technique and to evaluate the degradable properties of the stents in vitro.
METHODS: After synthesizing of PLGA (80:20), the electrospinning technique was used to fabricate the urethral graft. Crosslinking was carried out at a temperature of 4 ℃ using 1% glutaraldehyde for 24 hours. The morphology was investigated by scanning electron microscope. The tensile and anti-compression properties of the stents were tested. The stents were cut into pieces (1 cm in length) which were immersed in the urine to evaluate the degradability. The morphology, residual weight rate and molecular weight were investigated in each observation point.
RESULTS AND CONCLUSION: The stents had the nanostructure and the morphology satisfied the demand of a degradable urethral scaffold. The porosity was about 89%, and the pore size was (32±19) µm. The tensile and anti-compression properties had improved after crosslinking by glutaraldehyde. The residual weight rate curve of the stents was close to a straight line, and degraded completely within 10 weeks. The decrease of molecular weight was apparently in the early stage, and the decreasing was slow down in the later stage. The properties of PLGA (80:20) urethral stents fabricated by electrospinning technique and crosslinked by glutaraldehyde can fully satisfy the demand of a degradable urethral graft.

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