中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (42): 7817-7821.doi: 10.3969/j.issn.1673-8225.2011.42.007

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

可降解聚羟基丁酸酯-羟基戊酸酯的共聚物膜

陈建洪，唐  倩，吴  坚，梁焕友   

  1. 中山大学附属第三医院口腔科，广东省广州市  510630
  • 收稿日期:2011-05-03 修回日期:2011-06-01 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 唐倩，副主任医师，中山大学附属第三医院口腔科，广东省广州市 510630
  • 作者简介:陈建洪，男，1959年生，广东省兴宁市人，汉族，中山医科大学毕业，副主任医师，主要从事口腔内科研究。
  • 基金资助:

    广东省科技计划项目(2007B031500016)。

Biopolymer poly hydroxybutyrate-hydroxyvalerate membrane

Chen Jian-hong, Tang Qian, Wu Jian, Liang Huan-you   

  1. Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-05-03 Revised:2011-06-01 Online:2011-10-15 Published:2011-10-15
  • Contact: Tang Qian, Associate chief physician, Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China doodooer@126.com
  • About author:Chen Jian-hong, Associate chief physician, Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
  • Supported by:

    the Science and Technology Development Program of Guangdong Province, No. 2007B031500016*

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摘要：

背景：聚羟基丁酸/羟基戊酸共聚酯已用于构建心脏生物瓣膜，但不知是否可作为骨再生引导膜？
目的：观察聚羟基丁酸/羟基戊酸共聚酯膜的生物相容性，并评价其成骨能力。
方法：采用MTT法检测100%，75%，50%，25%聚羟基丁酸/羟基戊酸共聚酯材料浸提液对犬骨髓间充质干细胞相对增殖度的影响，并评价其细胞毒性；在犬胫骨缺损模型左右两侧远心端骨缺损上方覆盖聚羟基丁酸/羟基戊酸共聚酯膜作为实验组，在近心端仅以骨膜瓣复位作为对照组。
结果与结论：100%，75%，50%，25%聚羟基丁酸/羟基戊酸共聚酯膜的细胞毒性分级为0~1级，对骨髓间充质干细胞的生长及增殖无毒性；实验组术后第2周即可见新骨形成，12周时骨缺损区已完全被新骨充填，骨修复质量明显优于对照组。表明聚羟基丁酸/羟基戊酸共聚酯膜具有良好的生物相容性，对骨髓间充质干细胞无明显毒性，引导成骨能力强。

关键词: 聚羟基丁酸酯-羟基戊酸酯共聚物, 骨髓间充质干细胞, 生物相容性, 骨缺损, 引导骨组织再生

Abstract:

BACKGROUND: Poly hydroxybutyrate-hydroxyvalerate (PHBV) has been used to construct bioprosthetic heart valve. It remains unclear whether it can be used as membrane for guided bone regeneration.
OBJECTIVE: To investigate the biocompatibility of PHBV membrane and evaluate its efficiency of promoting bone regeneration in vivo.
METHODS: Effects of 100%, 75%, 50%, 25% PHBV extract solution on relative growth rate of dog bone marrow mesenchymal stem cells were measured by MTT method and cytotoxicity of the biomaterials was evaluated. Bone defects were made on distal bilateral tibias and treated with PHBV membrane; the proximal bilateral tibias undergoing reduction of periosteal flap and were used as control.
RESULTS AND CONCLUSION: The toxicity gradation of PHBV membranes was grade 0-1. That is, they were not toxic to growth and proliferation of bone marrow mesenchymal stem cells. New bone regeneration was observed in the defects covered with PHBV membranes at week 2 post-surgery. The defects covered with PHBV membranes were filled with mature bone at week 12 post-surgery. The bone repair in experimental groups was earlier and better than that in control groups. Results demonstrated that PHBV membrane, which has no cytotoxicity to mesenchymal stem cells in a wide range of extract concentration, could be a promising biopolymer membrane for guided bone regeneration.

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