中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7411-7415.doi: 10.3969/j.issn.1673-8225.2011.40.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

振动应力刺激骨髓间充质干细胞修复兔骨缺损

张  路，赵文志，何盛为，方  旭，米立东，孙雪刚，杜广宇，孙传秀   

  1. 大连医科大学附属二院北院骨外科，辽宁省大连市     116023
  • 收稿日期:2011-04-01 修回日期:2011-05-19 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 赵文志，博士，教授，主任医师，硕士生导师，主任，大连医科大学附属二院北院骨外科，辽宁省大连市116023 drzwz@163.com
  • 作者简介:张路★，男，1976年生，辽宁省大连市人，汉族，2008年大连医科大学毕业，硕士，主治医师，主要从事骨组织工程、骨与关节创伤研究。 andrew76aa@163.com
  • 基金资助:

    国家自然科学基金项目(30970708)，课题名称：低频振动刺激BMSCs修复骨缺损生物模型研究。

Effect of vibration stress stimulation on bone marrow stem cells for repairing rabbit bone defects

Zhang Lu, Zhao Wen-zhi, He Sheng-wei, Fang Xu, Mi Li-dong, Sun Xue-gang, Du Guang-yu, Sun Chuan-xiu   

  1. Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian  116023, Liaoning Province, China
  • Received:2011-04-01 Revised:2011-05-19 Online:2011-10-01 Published:2011-10-01
  • Contact: Zhao Wen-zhi, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China drzwz@163.com
  • About author:Zhang Lu★, Master, Attending physician, Department of Orthopedics, North Hospital, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China andrew76aa@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30970708*

PDF

338

被引次数

3

摘要：

背景：研究证实，力学因素可调控、诱导骨髓间充质干细胞定向分化为骨细胞，提高分化效率。
目的：观察振动应力刺激对兔骨缺损微环境中骨髓间充质干细胞移植修复肱骨骨缺损成骨分化能力的影响。
方法：24只兔按随机数字表法分为非振动单纯骨基质明胶组、非振动骨基质明胶+骨髓间充质干细胞复合植入组、振动骨基质明胶+骨髓间充质干细胞复合植入组，每组8只，建立兔肱骨骨缺损模型。振动组兔置于振动平台，以0.3 G的加速度，25 Hz，正弦波型，1次/d，30 min/次，持续4周施加振动刺激。
结果与结论：造模4周后，大体观察结果显示，振动组骨痂生长良好，组织学切片显示其新生骨量较多，可见大量成骨细胞，骨缺损与断端形成骨性连接；振动组Ⅰ型胶原蛋白、RUNX2 mRNA表达水平明显高于非振动组。提示振动应力刺激可促进骨缺损微环境中骨髓间充质干细胞的成骨分化能力，提高Ⅰ型胶原蛋白、RUNX2 mRNA表达水平，从而加速骨缺损修复的进程。

关键词: 振动刺激, 骨髓间充质干细胞, 骨缺损, RUNX2, Ⅰ型胶原

Abstract:

BACKGROUND: Mechanical factors can regulate and induce the differentiation of bone marrow mesenchymal stem cells (BMSCs) into bone cells, and increase the differentiation efficiency.
OBJECTIVE: To investigate the effect of vibration stress stimulation on the osteogenic differentiation ability of BMSCs in the bone defect microenvironment.
METHODS: Twenty-four rabbits were randomized into three groups: bone matrix gelatin (BMG), BMG+BMSCs, vibration+BMG+BMSCs groups. Humeral bone defect models were established in all the rabbits. Vibration stress stimuli were applied to live animals with 0.3 G acceleration, 25 Hz, sine wave type, 1 once a day, 30 minutes once, for 4 weeks.
RESULTS AND CONCLUSION: Gross observation display callus growth good in the vibration group, histological sections showed that the amount of new bone was large in the vibration group, and a large number of osteoblasts, bone formation and bone ends connected were found in the vibration group. mRNA expression of RUNX2 and type Ⅰ collagen was significantly higher in the vibration group than in the non vibration groups. Vibration can promote BMSCs, up-regulate RUNX2 gene, type Ⅰ collagen gene thus speeding up the repair of bone defects.

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