中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7149-7152.doi: 10.3969/j.issn.1673-8225.2011.38.028

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米载银磷酸锆抗菌聚氨酯的细胞毒性检测

邹兆伟，史福军，黄宗海，陈  飞，李  强，崔春晖   

  1. 南方医科大学珠江医院普外科，广东省广州市  510282
  • 收稿日期:2011-05-21 修回日期:2011-07-27 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 黄宗海，教授，博士生导师，南方医科大学珠江医院普外科，广东省广州市 510282 drhuangzh@ yahoo.com.cn
  • 作者简介:邹兆伟★，男，1985年生，江西省瑞金市人，汉族，南方医科大学在读硕士，主要从事人工肛门括约肌生物材料的研究。 360094442@ qq.com
  • 基金资助:

    2009年海珠区科技计划(智能型原位人工肛门的研制，2009-Y-017)；2009年广州市科技计划(智能型原位人工肛门的研制；2009Z1-E251)；2009年广东省科技计划(智能型纳米复合材料原位人工肛门的研制，2009A030200014)；2010年广东省产学研结合项目(抗菌型有机硅橡胶人工肛门封堵器的研制与开发，2010B090400423)。

Cytotoxicity of nano-silver-zirconium phosphate antibacterial polyurethane

Zou Zhao-wei, Shi Fu-jun, Huang Zong-hai, Chen Fei, Li Qiang, Cui Chun-hui   

  1. Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou  510280, Guangdong Province, China
  • Received:2011-05-21 Revised:2011-07-27 Online:2011-09-17 Published:2011-09-17
  • Contact: Huang Zong-hai, Professor, Doctoral supervisor, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China drhuangzh@ yahoo.com.cn
  • About author:Zou Zhao-wei★, Studying for master’s degree, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China 360094442@qq.com
  • Supported by:

    the Science and Technology Plan of Zhuhai in 2009, No. 2009-Y-017*; the Science and Technology Plan of Guangzhou City in 2009, No. 2009Z1-E251*; the Science and Technology Plan of Guangdong Province in 2009, No. 2009A030200014*; the Combination Project of Guangdong Province in 2010, No. 2010B090400423*

PDF

518

被引次数

14

摘要：

背景：聚氨酯是一种研究热门的医用高分子材料，并在许多人工器官和医疗装置中发挥着至关重要的作用。
目的：制备并观察纳米载银磷酸锆抗菌聚氨酯生物材料对L929细胞增殖活性的影响及细胞毒性反应。
方法：采用MTT法检测各组抗菌聚氨酯对传代培养小鼠成纤维细胞L929的细胞毒性，L929细胞接种于96孔板，随机分为3组进行培养，实验组用抗菌聚氨酯浸提液处理，阳性对照组用苯酚溶液，阴性对照组用高密度聚乙烯浸提液，于24，48，72 h后在倒置相差显微镜下观察细胞形态，通过MTT比色法检测各组细胞的相对增殖率，并评价材料的细胞毒性。
结果与结论：实验组作用于小鼠成纤维细胞24，48，72 h后，相对增殖率分别为94.3%~97.9%，94.5%~99.8%和90.8%~96.3%，材料细胞毒性评级为Ⅰ级。提示添加低浓度纳米载银无机抗菌剂RHA-2(添加比例<5%)的热塑性聚氨酯，无细胞毒性，符合医用生物材料安全标准。

关键词: 纳米载银无机抗菌剂, 聚氨酯, 细胞毒性, 医用高分子材料, 抗菌材料

Abstract:

BACKGROUND: Polyurethane as a popular medical polymer material plays a crucial role in many artificial organs and medical devices.
OBJECTIVE: To prepare nano-silver-zirconium phosphate antibacterial polyurethane and to observe its cytotoxic effect on L929 cells.
METHODS: L929 cells were incubated into 96-well plates and divided into three groups: experimental group treated with antibacterial polyurethane, positive control group with phenol solution, negative control group with high-density polyethylene extract. The cell morphology was observed under inverted phase contrast microscope after 24, 48 and 72 hours. The relative growth rate (RGR) was calculated and the cytotoxicity was graded by with MTT method.
RESULTS AND CONCLUSION: After cultured for 24, 48 and 72 hours, the RGR of L929 cells in the experimental group was 94.3%-97.9%, 94.5%-99.8% and 90.8%-96.3%, respectively. The cytotoxicity of the antibacterial polyurethanes was graded level Ⅰ. Antibacterial polyurethane has no cytotoxicity to L929 cells and shows good biocompatibility, meeting the safety standards of biomedical materials.

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